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Human Osteoblast Expression of Osteonectin on Different Charged Substrata

Published online by Cambridge University Press:  15 February 2011

M. Russell ,
L. Zou ,
H. Keeping ,
M. G. Ehrlich  and
W. R. Walsh
M. Russell
Affiliation:
Biomechanics Laboratory, Department of Orthopaedics, Rhode Island Hospital, Brown University School of Medicine.
L. Zou
Affiliation:
Biomechanics Laboratory, Department of Orthopaedics, Rhode Island Hospital, Brown University School of Medicine.
H. Keeping
Affiliation:
Biomechanics Laboratory, Department of Orthopaedics, Rhode Island Hospital, Brown University School of Medicine.
M. G. Ehrlich
Affiliation:
Biomechanics Laboratory, Department of Orthopaedics, Rhode Island Hospital, Brown University School of Medicine.
W. R. Walsh
Affiliation:
Biomechanics Laboratory, Department of Orthopaedics, Rhode Island Hospital, Brown University School of Medicine. Division of Engineering, Brown University, Providence, RI.
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Extract

Cellular interactions at the interface between bone and a prosthetic device plays an important role in total joint reconstruction success or failure. Aseptic loosening is a typical long-term complication encountered due to a failure at this interface. A number of factors have been shown to influence cell mitogenic and morphological behavior including load and stability [1] surface topography and surface chemistry [2]. The selection of suitable surface coatings on a prosthetic device would be facilitated if the nature of the interaction of bone cells to surface could be predicted from appropriate behavior of cells in tissue culture [3]. Molecular biology techniques now enable investigators to study cell-substrate interactions and gene expression at the mRNA level.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 331: Symposium T – Biomaterials for Drug and Cell Delivery , 1993 , 133
Copyright
Copyright © Materials Research Society 1994

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References

1. Brunuette, D M, Ratkay, J, and Chehroudi, B. Behavior of osteoblasts on micromachined surfaces. The Bone-Biomaterial Interface. Davies, J E, Ed.. University of Toronto Press, Toronto, 170–180 (1991).Google Scholar
2. Pizzoferrato, A, Crapetti, G, Stea, S, and Toni, A. Cellular events in the mechanisms of prosthesis loosening. Clinical Materials 7: 51–81 (1991).Google Scholar
3. Steele, J, McFarland, C, Dalton, B A, Johnson, G, Evans, M D M, Howlett, C R, and Underwood, P A. Attachment of human bone cells to tissue culture polystyrene and to unmodified polystyrene: the effect of surface chemistry upon initial cell attachment. In press.Google Scholar
4. Jundt, G, Berghauser, K H, Termine, J D, and Schulz, A. Osteonectin - a differentiation marker of bone cells. Cell Tissue Research 248: 0409–415 (1987).Google Scholar
5. Puleo, D A, Preston, K E, Shaffer, J B, and Bizios, R. Examination of osteoblast-orthopaedic biomaterial interactions using molecular techniques. Biomaterials 14 (2): 111–114 (1993).Google Scholar
6. Ibaraki, K, Termine, J D, Whitson, S W, and Young, M F. Bone matrix mRNA expression in differentiating fetal bovine osteoblasts. Journal of Bone and Mineral Research 7(7):743–754 (1992).Google Scholar
7. Aufmkolk, B, Hauschka, P V, and Schwartz, E R. Characterization of human bone cells in culture. Calcified Tissue Int 37: 228–235 (1985).Google Scholar
8. Chomczynski, P. and Sacchi, N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 162: 156–159 (1987).Google Scholar
9. Keeping, H S, Winters, S J, Attardi, B, and Troen, P. Developmental changes in testicular inhibin and androgen-binding protein during sexual maturation in the cynomolgus monkey, macaca fascicularis. Endocrinology 126: 2858–2867 (1990).Google Scholar
10. Villarreal, X C, Mann, K G, and Long, G L. Structure of human osteonectin based upon analysis of cDNA and genomic sequences. Biochemistry 28: 6483–6491 (1989).Google Scholar
11. Boskey, A.L. Mineral-matrix interactions in bone and cartilage. CORR 281: 244–274 (1992).Google Scholar
12. Klein-Soyer, C, Hemmendinger, S, and Cazenave, J P. Culture of human vascular endothelial cells on a positively charged polystyrene surface, Primaria: comparison with fibronectin-coated tissue culture grade polystyrene. Biomaterials 10: 85–90 (1989).Google Scholar