Hostname: page-component-7479d7b7d-68ccn Total loading time: 0 Render date: 2024-07-10T22:30:32.589Z Has data issue: false hasContentIssue false
  • English
  • Français

In Vitro Cell Adhesion and Detachment Behavior for Various Biomaterial Substrates

Published online by Cambridge University Press:  15 February 2011

Mary Beth Schmidt  and
John B. Brunski
Mary Beth Schmidt
Affiliation:
Rensselaer Polytechnic Institute, Department of Biomedical Engineering, Troy, NY 12180
John B. Brunski
Affiliation:
Rensselaer Polytechnic Institute, Department of Biomedical Engineering, Troy, NY 12180
Get access

Abstract

Cell-biomaterial interactions for different material compositions were evaluated through quantitative examination of in vitro cell spreading behavior and shear-induced cell detachment. Biomaterial substrates consisted of identically prepared, well characterized bulk samples of implant grade cp Ti, Ti-6AI-4V, and Synamel. 3T3 fibroblasts were seeded on the substrate surfaces and incubated for 2 hours. Adherent cell morphology was then examined using scanning electron microscopy and digital image analysis. The cell detachment response was measured using a parallel plate flow chamber. When categorized according to observed cell morphology, projected cell areas were found to be lognormally distributed for all biomaterials and ln(cell area) was significantly greater for Synamel, while no difference existed between cp Ti and Ti-6AI-4V. In addition, the average post-shear fraction of adherent cells was significantly greater for Synamel substrates, as compared to cp Ti and Ti-6AI-4V substrates. The results from this study indicate that in vitro cell adhesion and detachment behavior are measurably influenced by biomaterial substrate composition.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 252: Symposium T – Tissue-Inducing Biomaterials , 1991 , 193
Copyright
Copyright © Materials Research Society 1992

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

1. Johansson, C., Lausmaa, J., Ask, M., Hansson, H.-A., Albrektsson, T., J. Biomed. Eng. 11, 3 (1989).Google Scholar
2. Linder, L., Acta Orthop. Scand. 60 (2), 129 (1989).CrossRefGoogle Scholar
3. Johansson, C., Albrektsson, T., Intl. J. Oral Maxillofac. Impl. 2 (2), 69 (1987).Google Scholar
4. Cook, S.D., Kay, J.F., Thomas, K.A., Jarco, M., Intl. J. Oral Maxillofac. Impl. 2 (1), 15 (1987).Google Scholar
5. Magee, F.P., Kay, J.F., Hedley, A.K., Trans. Soc. Biomat. 1989, 173.Google Scholar
6. Gutowski, W., in Fundamentals of Adhesion, edited by Lee, L.-H. (Plenum Press, New York, 1991), pp. 123–131.Google Scholar
7. Frangos, J.A., Eskin, S.G., Mclntire, L.V., Ives, C.L., Science 227, 1477 (1985).CrossRefGoogle Scholar
8. Paddock, S.W., J. Cell Sci. 93, 143 (1989).Google Scholar
9. Rao, C.R., Advanced Statistical Methods in Biometric Research (John Wiley, New York, 1952), pp. 91–102.Google Scholar
10. Sokal, R.R., Rohlf, F.J., Biometry (W.H. Freeman, New York, 1981), pp. 716–721.Google Scholar
11. Johnson, R.E., Dettre, R.H., in Surface and Colloid Science 2, edited by Matijevic, E. (Wiley-Interscience, New York, 1969), pp. 98–106.Google Scholar
12. Jansen, J.A., Wolters-Lutgerhorst, J.M.L., Wijn, J.R. De, Mullem, P.J. Van, in Biomaterials and Biomechanics 1983, edited by Ducheyne, P., Perre, G. Van der, Aubert, A.E. (Elsevier Science, Amsterdam, 1984), pp. 259–264.Google Scholar
13. Schakenraad, J.M., Busscher, H.J., Wildevuur, C.R.H., Arends, J., in Biological Performance of Biomaterials, edited by Christel, P., Meunier, A., Lee, A.J.C. (Elsevier Science, Amsterdam, 1986), pp. 263–268.Google Scholar
14. Schakenraad, J.M., Busscher, H.J., Wildevuur, C.R.H., Arends, J., J. Biomed. Mater. Res. 20, 773 (1986).Google Scholar
15. Bardsley, W.G., Alpin, J.D., J. Cell Sci. 61, 365 (1983).CrossRefGoogle Scholar
16. Ushida, T., Tateishi, T., Moriya, N., in Clinical Implant Materials (Advances in Biomaterials, Volume 9), edited by Heimke, G., Soltesz, U., Lee, A.J.C., (Elsevier Science, Amsterdam, 1990), pp. 231–263.Google Scholar
17. Truskey, G.A., Proulx, T.L., Biotechnol. Prog. 6 (6), 513 (1990).CrossRefGoogle Scholar