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Layer-by-Layer Engineered Microreactors for Bio-Polymerization of 4-(2-aminoethyl) phenol hydrochloride

Published online by Cambridge University Press:  01 February 2011

R. Ghan ,
T. Shutava ,
A. Patel ,
V. John  and
Y. Lvov
R. Ghan
Affiliation:
Institute for Micromanufacturing, Louisiana Tech University, Ruston, LA, 71270
T. Shutava
Affiliation:
Institute for Micromanufacturing, Louisiana Tech University, Ruston, LA, 71270
A. Patel
Affiliation:
Institute for Micromanufacturing, Louisiana Tech University, Ruston, LA, 71270
V. John
Affiliation:
Department of Chemical Engineering, Tulane University, New Orleans, LA 70118
Y. Lvov
Affiliation:
Institute for Micromanufacturing, Louisiana Tech University, Ruston, LA, 71270
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Abstract

This study presents the results of polymerization of phenol to yield fluorescent polymer encapsulated within shells fabricated via layer-by-layer (L-b-L) assembly. Hollow polyelectrolyte microcapsules (shells) were prepared using weakly cross-linked melamine formaldehyde (MF) particles. Dissolution of the MF cores was achieved by changing the pH of the solution. Horseradish peroxidase (HRP), the catalyzing enzyme, was loaded in these capsules by taking advantage of the “open/close” mechanism of the capsules by altering the pH. The empty shells were then suspended in a concentrated solution of monomer. Since the monomer is a low molecular weight species, it freely permeates through the polyion wall into the shells. Addition of aliquots of hydrogen peroxide initiated the polymerization reaction and the polymer formed from the ensuing reaction was confined in the shells due to its high molecular weight. The model used for demonstrating this synthesis is polymerization of 4-(2-aminoethyl) phenol hydrochloride commonly known as tyramine hydrochloride to its corresponding polymeric form by reacting it with hydrogen peroxide. Fluorescence spectrometry (FS), confocal laser scanning microscopy (CLSM), and atomic force microscopy (AFM) were the characterization methods employed to confirm polymerization in situ shells.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 782: Symposium A – Micro- and Nanosystems , 2003 , A5.43
Copyright
Copyright © Materials Research Society 2004

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References

REFERENCES

1. Banerjee, S., Premchandran, R., Tata, M., John, V.T., McPherson, G.L., Ind. Eng. Chem. Res., 35 pp. 3100, (1996)Google Scholar
2. Fernandes, N.E., Fisher, S.M., Poshuta, J.C., Vlachos, D.G., Chem. Mater., 13(6), pp. 2023, (2001)Google Scholar
3. Tung, C., Wu, L., Zhang, L., Chen, B., Acc. Chem. Res., 36 pp. 39, (2003)Google Scholar
4. Lvov, Y., Ariga, K., Ichinose, I., Kunitake, T., J. Am. Chem. Soc., 117 pp. 6117, (1995)Google Scholar
5. Sukhorukov, G., “Multilayer Hollow Microspheres,” Dendrimers, Citus Books, NY, (2002), 5, pp. 111 Google Scholar
6. Lvov, Y., Antipov, A., Mamedov, A., Möhwald, H., Sukhorukov, G., Nano Letters, 1 pp. 125, 2001 Google Scholar
7. Tiourina, O., Antipov, A., Sukhorukov, G., Lvov, Y., Möhwald, H., Macromolecular Bioscience, 1 pp. 209, 2001 Google Scholar
8. Gao, C., Liu, X., Shen, J., Mohwald, H., Chem. Comm., pp. 1928, 2001 Google Scholar
9. Antipov, A., Sah, S., Sukhorukov, G., Lvov, Y., Bioconjugate Chemistry 2004 (submitted and accepted for publication)Google Scholar
10. Balabushevitch, N.G., Sukhorukov, G.B., Moroz, N.A., Volodkin, D.V., Larionova, N.I., Donath, E., Mohwald, H., Biotechnol. Bioeng. 76, pp. 207, 2001 Google Scholar
11. Antipov, A.A., Sukhorukov, G.B., Leporatti, S., Radtchenko, I.L., Donath, E. and Mohwald, H., Colloids and Surfaces A, 2002, 200, pp. 535, 2002 Google Scholar
12. Ibarz, G, Dahne, L, Donath, E, Mohwald, H, Advanced Materials, 13 pp. 1324, 2001 Google Scholar
13. Shchukin, DG, Radtchenko, IL, Sukhorukov, G.B., Materials Letters, 57 (11), pp. 1743, 2003 Google Scholar
14. Shchukin, DG, Radtchenko, IL, Sukhorukov, G.B., J Phys Chem B, 107 (1), pp. 86, 2003 Google Scholar
15. Sukhorukov, GB, Susha, AS, Davis, S, Leporatti, S, Donath, E, Hartmann, J, Mohwald, H., J.Colloid Interface Science, 247 (1), pp. 251, 2002 Google Scholar
16. Sophiannikova, M., Radchenko, I., Sukhorukov, G., Shchukin, D., Yakimansky, A., Ilnytskyi, Y., J Chem. Phys., 118 pp. 9007, 2003 Google Scholar
17. Buchanan, I.D., Nicell, J.A., J.Chem. Technol. Biotechnol., 72 pp. 23, 1998 Google Scholar
18. Buchanan, I.D., Nicell, J.A., J.Chem. Technol. Biotechnol., 74 pp. 669, 1999 Google Scholar
19. Spell, E.J.M. Hopman, A.H.N., Komminoth, P, J. Histochem. Cytochem., 47(3), pp. 281, 1999 Google Scholar
20. Sukhorukov, G.B., Antipov, A.A., Voigt, A., Donath, E., and Möhwald, H., Macromol. Rapid Commun., 22 pp. 44, 2001 Google Scholar
21. Townshend, A., Li, Y.-Z., Analytica Chimica Acta, 359(1–2), pp. 149, 1998 Google Scholar
22. Alva, K.S., Kuma, J., Marx, K.A., Tripathy, S.K., Macromolecules, 30 pp. 4024, 1997 Google Scholar