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The Mechanism of Chitosan Enhanced Lung Surfactant Adsorption at the Air-Liquid Interface in the Presence of Serum Proteins

Published online by Cambridge University Press:  01 February 2011

Patrick C Stenger ,
Omer M Palazoglu  and
Joseph A Zasadzinski
Patrick C Stenger
Affiliation:
psteng@engineering.ucsb.edu, University of California, Santa Barbara, Department of Chemical Engineering, Engineering 2, Room 3357, Santa Barbara, CA, 93106-5080, United States
Omer M Palazoglu
Affiliation:
omerp@umail.ucsb.edu, University of California, Department of Chemical Engineering, Santa Barbara, CA, 93106-5080, United States
Joseph A Zasadzinski
Affiliation:
gorilla@engineering.ucsb.edu, University of California, Department of Chemical Engineering, Santa Barbara, CA, 93106-5080, United States
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Abstract

Pressure-area isotherms and fluorescence microscopy were used to investigate the impact of chitosan on the competitive adsorption between lung surfactant (LS) and serum proteins at the air-liquid interface. Isotherms demonstrate an optimum chitosan concentration to mediate LS adsorption; higher concentrations actually reduce the amount of LS which can adsorb. Fluorescence microscopy images show the transition from a serum protein to LS-covered interface for the optimum chitosan concentration; this transition goes through a sharply phase separated coexistence region. The results suggest that the cationic chitosan molecules mediate adsorption of the negatively charged LS aggregates by reducing the electrostatic barrier imposed by negatively charged interfacial serum proteins.

Keywords

adsorptionbiomedicalbiomaterial
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1061: Symposium MM – Biomolecular and Biologically Inspired Interfaces and Assemblies , 2007 , 1061-MM10-03
Copyright
Copyright © Materials Research Society 2008

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References

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