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Study on the Effects of Type I Collagen Combined with Noncollagenous Proteins on Hydroxyapatite Formation in vitro using SPM and GIXD

Published online by Cambridge University Press:  31 January 2011

Xiaolan Ba ,
Elaine DiMasi  and
Miriam H Rafailovich
Xiaolan Ba
Affiliation:
xba@ic.sunysb.edu, Stony Brook University, Materials Science and Engineering, Stony Brook, New York, United States
Elaine DiMasi
Affiliation:
dimasi@bnl.gov, Brookhaven National Laboratory, National Synchrotron Light Source, Upton, New York, United States
Miriam H Rafailovich
Affiliation:
mrafailovich@notes.cc.sunysb.edu, Stony Brook University, Materials Science and Engineering, Stony Brook, New York, United States
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Abstract

The effects of the components of extracellular matrix on the bone formation and the kinetics of crystal growth of calcium phosphate have remained unknown. In this paper, we reported a method to investigate the role of Type I collagen and the interactions with other ECM proteins such as fibronectin and elastin during biomimic mineralization process in vitro. The early stage of mineralization was characterized by scanning probe microscopy (SPM) and shear modulation force microscopy (SMFM). The late stage of mineralization was investigated by synchrotron grazing incident x-ray diffraction (GIXD). The results demonstrate the cooperative interaction between type I collagen and noncollagenous proteins such as fibronectin or elastin could be essential for the biomineralization.

Keywords

x-ray diffraction (XRD)scanning probe microscopy (SPM)biomimetic (assembly)
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1238: Symposium UU – Molecular Biomimetics and Materials Design , 2009 , 1238-UU03-05
Copyright
Copyright © Materials Research Society 2010

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