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An efficient uric acid biosensor based on tin oxide thin film matrix

Published online by Cambridge University Press:  02 April 2013

Kashima Arora ,
Monika Tomar  and
Vinay Gupta
Kashima Arora
Affiliation:
Department of Physics and Astrophysics, University of Delhi, Delhi 110007, India
Monika Tomar
Affiliation:
Physics Department, Miranda House, University of Delhi, Delhi 110007, India
Vinay Gupta
Affiliation:
Department of Physics and Astrophysics, University of Delhi, Delhi 110007, India
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Abstract

Uric acid biosensor has been developed using tin oxide (SnO2) thin film. The electrochemistry of the developed thin film based electrode is investigated by using cyclic voltammetry. The obtained results demonstrate that the semiconducting SnO2 matrix offers a striking electrocatalytic activity to the immobilized uricase towards the oxidation of uric acid and promotes the transfer of electrons from the active sites of enzyme onto the electrode. SnO2 thin film matrix gives a high sensitivity of 0.2 mA/mM and a shelf life of 20 weeks. Moreover, SnO2 electrode exhibits excellent selectivity and outstanding analytical stability and reproducibility, which enables a reliable and selective determination of uric acid. The SnO2 based uric acid biosensor shows a linear variation in a wide range from 0 to 1.0 mM of uric acid concentration and the Michaelis Menten Constant (Km) is estimated to be 0.28 mM which indicated the high affinity of uricase towards its analyte (uric acid). The results indicate that the SnO2 thin film matrix offers a new and promising platform for the development of novel biosensors.

Keywords

sputteringx-ray diffraction (XRD)sensor
Type
Articles
Information
MRS Online Proceedings Library (OPL) , Volume 1530: Symposium XX – Materials and Concepts for Biomedical Sensing , 2013 , mrsf12-1530-xx03-14
Copyright
Copyright © Materials Research Society 2013

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References

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