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Comparison of Proliferation and Growth of Human Keratinocytes on Plasma CO-Polymers of Acrylic acid/1,7- Octadiene and Self Assembled Monolayers

Published online by Cambridge University Press:  10 February 2011

D. B. Haddow ,
R. M. France ,
R. D. Short ,
S. Macneil  and
R. A. Dawson
D. B. Haddow
Affiliation:
Department of Engineering Materials, The University of Sheffield, Sheffield S1 3JD, U.K., d.b.haddow@sheffield.ac.uk
R. M. France
Affiliation:
Department of Engineering Materials, The University of Sheffield, Sheffield S1 3JD, U.K., d.b.haddow@sheffield.ac.uk
R. D. Short
Affiliation:
Department of Engineering Materials, The University of Sheffield, Sheffield S1 3JD, U.K., d.b.haddow@sheffield.ac.uk
S. Macneil
Affiliation:
Department of Medicine, Clinical Sciences Centre, University of Sheffield, Northern General Hospital, U.K.
R. A. Dawson
Affiliation:
Department of Medicine, Clinical Sciences Centre, University of Sheffield, Northern General Hospital, U.K.
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Abstract

Human keratinocytes have been cultured on plasma co-polymers (PCPs), self assembled monolayers (SAMs), tissue culture poly(styrene) (TCPS) and collagen I. The degree of keratinocyte attachment was measured over 24 hours and cell proliferation and growth monitored over 7 days using optical microscopy and DNA concentrations. Cell attachment and proliferation and growth on the PCP surfaces were compared with 2 self assembled monolayer (SAM) systems. PCP surfaces containing carboxylic acid functionalities promoted keratinocyte attachment, with optimum attachment levels seen on surfaces containing less than 5% acid groups. The level of attachment on these surfaces was comparable to that seen on collagen I, a preferred substratum for the culturing of keratinocytes. After several days in culture the cells were well attached and proliferative. Keratinocytes attached well to acidterminated SAMs but attached poorly to methyl-terminated SAMs.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 544: Symposium Y – Plasma Deposition and Treatment of Polymers , 1998 , 39
Copyright
Copyright © Materials Research Society 1999

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