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On-chip Detection of Chemiluminescent Biomolecules Using an Integrated Thin Film Silicon Photodiode
Published online by Cambridge University Press: 01 February 2011
Abstract
A hydrogenated amorphous silicon photodetector on a glass substrate is used for the integrated, real-time detection of chemiluminescent reactions in solution. The light emission at 425 nm resulting from the horseradish peroxidase-catalyzed oxidation of luminol is detected by a p-i-n amorphous silicon photodiode operated at zero bias. The limit of detection of the present device is in the range of nanomole of horseradish peroxidase per liter.
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