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Evaluation of RFLP analysis of the classification of selected black aspergilli

Published online by Cambridge University Press:  01 July 1997

LUCIE PAŘENICOVÁ ,
JACQUES A. E. BENEN ,
ROBERT A. SAMSON  and
JAAP VISSER
LUCIE PAŘENICOVÁ
Affiliation:
Section Molecular Genetics of Industrial Microorganisms, Wageningen Agricultural University, Dreijenlaan 2, 6703 HA Wageningen, The Netherlands
JACQUES A. E. BENEN
Affiliation:
Section Molecular Genetics of Industrial Microorganisms, Wageningen Agricultural University, Dreijenlaan 2, 6703 HA Wageningen, The Netherlands
ROBERT A. SAMSON
Affiliation:
Centraalbureau voor Schimmelcultures, P.O. Box 273, NL 3740 AG Baarn, The Netherlands
JAAP VISSER
Affiliation:
Section Molecular Genetics of Industrial Microorganisms, Wageningen Agricultural University, Dreijenlaan 2, 6703 HA Wageningen, The Netherlands
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Abstract

Twenty-one Aspergillus strains representing different A. awamori, A. phoenicis and A. foetidus isolates were studied in order to explore the potential of a fast RFLP analysis to identify fungal strains. The patterns were compared with those characteristic for A. niger, A. tubingensis, A. carbonarius, A. japonicus and A. aculeatus represented by A. niger CBS 120.49, A. tubingensis NW 756, A. carbonarius CBS 111.26, A japonicus CBS 114.51 and A. aculeatus CBS 101.43 and also with those of the type strains of A. heteromorphus CBS 117.55 and A. ellipticus CBS 707.79.

Sma I digested chromosomal DNA revealed characteristic rDNA patterns after ethidium bromide staining which were used in combination with hybridization patterns of Pst I/Sal I double digested chromosomal DNA with well-defined probes. This allowed clear distinction of eight separate species within the Aspergillus sect. Nigri group. The probes used were a 0·9 kb fragment of the 28S rDNA from Agaricus bisporus, an internal fragment of the pkiA gene from A. nidulans, and the pelA gene from A. niger. All the strains examined including those indicated as A. awamori and A. phoenicis were shown to belong either to A. niger, A. tubingensis or a group representing isolates of A. foetidus varieties, amongst which A. foetidus var. acidus CBS 564.65 and A. foetidus var. pallidus CBS 565.65.

Type
Research Article
Information
Mycological Research , Volume 101 , Issue 7 , July 1997 , pp. 810 - 814
Copyright
The British Mycological Society 1997

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