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Genetic diversity shown in Trichoderma biocontrol isolates

Published online by Cambridge University Press:  13 August 2004

M. Rosa HERMOSA ,
Emma KECK ,
Isabel CHAMORRO ,
Belén RUBIO ,
Luis SANZ ,
Juan A. VIZCAÍNO ,
Isabel GRONDONA  and
Enrique MONTE
M. Rosa HERMOSA
Affiliation:
Centro Hispano-Luso de Investigaciones Agrarias, Departamento de Microbiología y Genética, Universidad de Salamanca, Edificio Departamental lab 208, Avenida Campo Charro s/n, 37007 Salamanca, Spain. E-mail: emv@usal.es
Emma KECK
Affiliation:
Centro Hispano-Luso de Investigaciones Agrarias, Departamento de Microbiología y Genética, Universidad de Salamanca, Edificio Departamental lab 208, Avenida Campo Charro s/n, 37007 Salamanca, Spain. E-mail: emv@usal.es
Isabel CHAMORRO
Affiliation:
Centro Hispano-Luso de Investigaciones Agrarias, Departamento de Microbiología y Genética, Universidad de Salamanca, Edificio Departamental lab 208, Avenida Campo Charro s/n, 37007 Salamanca, Spain. E-mail: emv@usal.es
Belén RUBIO
Affiliation:
Centro Hispano-Luso de Investigaciones Agrarias, Departamento de Microbiología y Genética, Universidad de Salamanca, Edificio Departamental lab 208, Avenida Campo Charro s/n, 37007 Salamanca, Spain. E-mail: emv@usal.es
Luis SANZ
Affiliation:
Centro Hispano-Luso de Investigaciones Agrarias, Departamento de Microbiología y Genética, Universidad de Salamanca, Edificio Departamental lab 208, Avenida Campo Charro s/n, 37007 Salamanca, Spain. E-mail: emv@usal.es
Juan A. VIZCAÍNO
Affiliation:
Centro Hispano-Luso de Investigaciones Agrarias, Departamento de Microbiología y Genética, Universidad de Salamanca, Edificio Departamental lab 208, Avenida Campo Charro s/n, 37007 Salamanca, Spain. E-mail: emv@usal.es
Isabel GRONDONA
Affiliation:
Newbiotechnic S.A. (NBT), Av. Américo Vespucio 69, 41092 Seville, Spain.
Enrique MONTE
Affiliation:
Centro Hispano-Luso de Investigaciones Agrarias, Departamento de Microbiología y Genética, Universidad de Salamanca, Edificio Departamental lab 208, Avenida Campo Charro s/n, 37007 Salamanca, Spain. E-mail: emv@usal.es
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Abstract

Genetic variability within 69 biocontrol isolates of Trichoderma, obtained from different geographical locations and culture collections and selected as biocontrol agents, was studied. Sequence data, obtained from the ITS1 region of rDNA and a fragment of the translation elongation factor 1 (tef1) gene, were used in a phylogenetic analysis. Phylograms showing similar topologies were generated using alignments containing the ITS1 region or a portion of the tef1 gene. 21 distinct ITS1 sequence types and 17 distinct tef1 sequence types were identified among the 69 isolates. More than 50% of the potential biocontrol strains were grouped within Trichoderma sect. Pachybasium; of these, 81% were grouped within the cluster that included the ex-type strains of T. harzianum and T. inhamatum, and 16% were grouped with T. virens. Within T. sect. Trichoderma, which included 36% of the 69 strains, 56% were grouped with T. asperellum, and 24% with T. viride, T. atroviride or T. koningii. Only 10% of the strains studied were located in T. sect. Longibrachiatum.

Type
Research Article
Information
Mycological Research , Volume 108 , Issue 8 , August 2004 , pp. 897 - 906
Copyright
The British Mycological Society 2004

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