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Partial retrotransposon-like DNA sequence in the genomic clone of Aspergillus flavus, pAF28

Published online by Cambridge University Press:  12 August 2003

Patricia A. OKUBARA ,
Brian K. TIBBOT ,
Alice S. TARUN ,
Cesaria E. McALPIN  and
Sui-Sheng T. HUA
Patricia A. OKUBARA
Affiliation:
USDA, Agricultural Research Service, Western Regional Research Center, 800 Buchanan Street, Albany, CA 94710, USA. E-mail: ssth@pw.usda.gov Current address: USDA ARS, Root Disease and Biological Control Research Unit, P.O. Box 646430, Washington State University, Pullman, WA 99164-6430, USA.
Brian K. TIBBOT
Affiliation:
USDA, Agricultural Research Service, Western Regional Research Center, 800 Buchanan Street, Albany, CA 94710, USA. E-mail: ssth@pw.usda.gov
Alice S. TARUN
Affiliation:
USDA, Agricultural Research Service, Western Regional Research Center, 800 Buchanan Street, Albany, CA 94710, USA. E-mail: ssth@pw.usda.gov
Cesaria E. McALPIN
Affiliation:
USDA, Agricultural Research Service, National Center for Agricultural Utilization Research, Peoria, IL, USA.
Sui-Sheng T. HUA
Affiliation:
USDA, Agricultural Research Service, Western Regional Research Center, 800 Buchanan Street, Albany, CA 94710, USA. E-mail: ssth@pw.usda.gov
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Abstract

A genomic clone of the aflatoxin-producing fungus Aspergillus flavus, designated pAF28, has been used as a probe for Southern blot fingerprinting of fungal strains. A large number of A. flavus strains isolated from corn fields and tree-nut orchards can be distinguished because the DNA fingerprint patterns are highly polymorphic. We have completed the sequencing of a 6355 bp insert in pAF28. The sequence features motifs and open reading frames characteristic of transposable elements of the gypsy class. We have named this new element AfRTL-1, for A. flavusretrotransposon-like DNA.

Type
Research Article
Information
Mycological Research , Volume 107 , Issue 7 , July 2003 , pp. 841 - 846
Copyright
© The British Mycological Society 2003

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