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Cloning of a novel gene encoding a C2H2 zinc finger protein that alleviates sensitivity to abiotic stresses in Aspergillus nidulans

Published online by Cambridge University Press:  01 August 2002

John D. O'NEIL ,
Marcin BUGNO ,
Michele S. STANLEY ,
Julia B. BARHAM-MORRIS ,
Nicola A. WOODCOCK ,
Darren J. CLEMENT ,
Nicholas J. W. CLIPSON ,
Michael P. WHITEHEAD ,
Daron A. FINCHAM  and
Paul HOOLEY
John D. O'NEIL
Affiliation:
School of Applied Sciences, University of Wolverhampton, Wulfruna Street Wolverhampton WVI ISB, UK.
Marcin BUGNO
Affiliation:
School of Applied Sciences, University of Wolverhampton, Wulfruna Street Wolverhampton WVI ISB, UK.
Michele S. STANLEY
Affiliation:
School of Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK.
Julia B. BARHAM-MORRIS
Affiliation:
School of Applied Sciences, University of Wolverhampton, Wulfruna Street Wolverhampton WVI ISB, UK.
Nicola A. WOODCOCK
Affiliation:
School of Applied Sciences, University of Wolverhampton, Wulfruna Street Wolverhampton WVI ISB, UK.
Darren J. CLEMENT
Affiliation:
Keele University School of Postgraduate Medicine, Thornburrow Drive, Hartshill, Stoke on Trent, Staffordshire ST4 7QB, UK.
Nicholas J. W. CLIPSON
Affiliation:
Department of Industrial Microbiology, University College Dublin, Belfield, Dublin 4, Eire.
Michael P. WHITEHEAD
Affiliation:
School of Applied Sciences, University of Wolverhampton, Wulfruna Street Wolverhampton WVI ISB, UK.
Daron A. FINCHAM
Affiliation:
School of Applied Sciences, University of Wolverhampton, Wulfruna Street Wolverhampton WVI ISB, UK.
Paul HOOLEY
Affiliation:
School of Applied Sciences, University of Wolverhampton, Wulfruna Street Wolverhampton WVI ISB, UK.
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Abstract

We report the cloning and sequencing of a DNA fragment encoding a putative C2H2 zinc finger protein from Aspergillus nidulans. The gene was isolated by complementation cloning of a salt sensitive phenotype of the A. nidulans sltA1 mutant. A 3·8 kb PstI fragment that restored wild type salt tolerance contained one large open reading frame of 2202 bp. The predicted protein (StzA) from this reading frame comprises 698 amino acids and has three Zinc fingers along with a putative transcriptional activation domain rich in acidic amino acids. The corresponding sequence from a sltA1 mutant contains a premature STOP codon resulting in loss of the putative transcriptional activator in the C-terminal region. The Zinc fingers show conserved motifs with a number of transcription factors including CreA from A. nidulans and the human Wilm's tumour susceptibility protein WT-1.

Type
Research Article
Information
Mycological Research , Volume 106 , Issue 4 , April 2002 , pp. 491 - 498
Copyright
© The British Mycological Society 2002

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