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Alteration in mononuclear cell subpopulations in dogs immunized with gentamicin-attenuated Leishmania infantum

Published online by Cambridge University Press:  21 August 2012

HAMID DANESHVAR ,
FARNAZ SEDGHY ,
SHAHRIAR DABIRI ,
HOSSEIN KAMIABI ,
MOHAMMAD M. MOLAEI ,
STEPHEN PHILLIPS  and
RICHARD BURCHMORE
HAMID DANESHVAR*
Affiliation:
Research Center of Tropical and Infectious Diseases, Kerman University of Medical Sciences, Kerman, Iran
FARNAZ SEDGHY
Affiliation:
Immunology Department, Kerman Medical University, Kerman, Iran
SHAHRIAR DABIRI
Affiliation:
Surgical Pathology Department, Afzalipoor Hospital, Kerman Medical University, Kerman, Iran
HOSSEIN KAMIABI
Affiliation:
Leishmaniasis Research Centre, Kerman Medical University, Kerman, Iran
MOHAMMAD M. MOLAEI
Affiliation:
Veterinary Medical School, Shahid Bahonar University of Kerman, Kerman, Iran
STEPHEN PHILLIPS
Affiliation:
Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow G12 8QQ, UK
RICHARD BURCHMORE
Affiliation:
Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow G12 8QQ, UK
*
*Corresponding author: Research Center of Tropical and Infectious Diseases, Kerman University of Medical Sciences, Kerman, Iran. Tel: 0098 9133403912. E-mail: h.daneshvar@bio.gla.ac.uk
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Summary

The impact of immunization with gentamicin-attenuated Leishmania infantum (H-line) on the immunophenotypic profile of popliteal lymph node (PLN) and peripheral blood mononuclear cells (PBMCs) of dogs was assessed by flow cytometry and immunohistochemistry. Compared with the dogs infected with L. infantum wild-type (Group WT), there was a significantly higher percentage of CD4+, CD44+ T cells and CD14+, MHC-II+ cells and a lower percentage of CD4+ CD25+ regulatory T cells in PLN of the immunized dogs with L. infantum H-line (Group H). The percentage of CD4+ and CD8+ T cells in PBMCs of immunized dogs was higher than that in dogs of Group WT. The CD4:CD8 ratio in PLN of dogs of Group H was significantly higher than that in dogs of Group WT. A significantly higher percentage of CD21+ B cells and a lower percentage of CD79b+ cells were found in PLN of the immunized dogs compared with dogs of Group WT. Immunohistochemical investigation showed no parasites in the PLN of immunized dogs whereas there were parasites in the PLN of 60% of dogs infected with L. infantum WT. In this study, the immunophenotypic profile of mononuclear cells of the immunized dogs correlates with cellular immunity.

Type
Research Article
Information
Parasitology , Volume 139 , Issue 13 , November 2012 , pp. 1689 - 1696
Copyright
Copyright © Cambridge University Press 2012

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