Hostname: page-component-6d856f89d9-vrt8f Total loading time: 0 Render date: 2024-07-16T06:28:53.990Z Has data issue: false hasContentIssue false
  • English
  • Français

An immunodominant antigen on the Dictyocaulus viviparus L3 sheath surface coat and a related molecule in other strongylid nematodes

Published online by Cambridge University Press:  06 April 2009

J. S. Gilleard ,
J. L. Duncan  and
A. Tait
J. S. Gilleard
Affiliation:
Department of Veterinary Parasitology, University of Glasgow, Bearsden Road, Glasgow G61 1QH
J. L. Duncan
Affiliation:
Department of Veterinary Parasitology, University of Glasgow, Bearsden Road, Glasgow G61 1QH
A. Tait
Affiliation:
Department of Veterinary Parasitology, University of Glasgow, Bearsden Road, Glasgow G61 1QH
Get access Rights & Permissions [Opens in a new window]

Summary

Monoclonal antibody 2A6 binds to the surface of the Dictyocaulus viviparus L3 sheath and identifies a molecule which is highly antigenic in both infected and vaccinated cattle. Immunogold electron microscopy reveals that the antibody binds to a highly stable surface coat overlying the epicuticle of the L3 sheath. The binding to the cuticular surface is stage specific, being restricted to the surface of the L3 sheath, although the antigen can be detected on Western blots of parasite homogenates from the L1 stage. Immunofluorescence on live parasites demonstrates that the antibodies also bind to the L3 sheath surface of many other strongylid nematodes. The antigen detected by 2A6 differs in molecular weight between the species; in D. viviparus the antigen is poorly resolved between 29 and 40 kDa by SDS-PAGE whereas in nematodes from the family Trichostrongyloidea a single band or doublet of approximately 20 kDa is detected, suggesting that a related molecule is present in these species. Although infection and vaccination with D. viviparus elicits a strong immune response to the L3 sheath antigen this does not appear to be the case following infection with other strongylid nematodes.

Keywords

Dictyocaulus viviparusstrongylid nematodecuticlesheathmonoclonal antibodiessurface coat
Type
Research Article
Information
Parasitology , Volume 111 , Issue 2 , August 1995 , pp. 193 - 200
Copyright
Copyright © Cambridge University Press 1995

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Bird, A. F. (1955). Importance of proteases as factors involved in the exsheathment mechanism of infective nematode larvae of sheep. Science 121, 107.CrossRefGoogle ScholarPubMed
Blaxter, M. L., Page, A. P., Rudin, W. & Maizels, R. M. (1992). Nematode surface coats: actively evading immunity. Parasitology Today 8, 243–7.CrossRefGoogle ScholarPubMed
Britton, C. (1991). Immunochemical analysis of antigens of the bovine lungworm Dictyocaulus viviparus. Ph.D. thesis, University of Glasgow.Google Scholar
Carlow, C. K. S., Franke, E. D., Lowrie, R. C., Portono, F. & Philipp, M. (1987). Monoclonal antibodies to a unique surface epitope of the human filariae Brugia malayi identifies infective larvae in mosquito vectors. Proceedings of the National Academy of Sciences, USA 84, 6914–18.CrossRefGoogle ScholarPubMed
Ellenby, C. (1968). Desiccation survival of the infection larvae of Haemonchus contortus. Journal of Experimental Biology 49, 469–75.CrossRefGoogle ScholarPubMed
Gilleard, J. S. (1992). An immunochemical analysis of Dictyocaulus viviparus infective larvae. Ph.D. thesis, Glasgow University.Google Scholar
Gilleard, J. S., Duncan, J. L. & Tait, A. (1995). Dictyocaulus viviparus: surface antigens of the L3 cuticle and sheath. Experimental Parasitology 80 (in the press).CrossRefGoogle ScholarPubMed
Glascodine, J., Tetley, L., Tait, A., Brown, D. & Shiels, B. (1990). Developmental expression of a Theileria annulata merozoite surface antigen. Molecular and Biochemical Parasitology 40, 105–12.CrossRefGoogle ScholarPubMed
Jarrett, W. F. H., Jennings, F. W., McIntyre, W. I. M., Mulligan, W., Thomas, B. A. & Urquhart, G. M. (1959). Immunological studies on D. viviparus infection. The immunity resulting from experimental infection. Immunology 2, 252–61.Google Scholar
Jarrett, W. F. H., Jennings, F. W., McIntyre, W. I. M., Mulligan, W. & Urquhart, G. M. (1960). Immunological studies on Dictyocaulus viviparus infection. Immunity produced by the administration of irradiated larvae. Immunology 3, 145–51.Google ScholarPubMed
Laemmli, U. K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227, 680–5.CrossRefGoogle ScholarPubMed
Murrell, K. D. & Graham, C. E. (1983). Shedding of antibody complexes by Strongyloides ratti (Nematoda) larvae. Journal of Parasitology 69, 70–3.CrossRefGoogle ScholarPubMed
Politz, S. M. & Phillip, M. (1992). Caenorhabditis elegans as a model for parasitic nematodes: a focus on the nematode cuticle. Parasitology Today 8, 6–12.Google Scholar
Prichard, D. I., McKean, P. G., Tighe, P. J. & Quinnell, R. J. (1991). Immunology, biochemistry and molecular biology of hookworm antigens. In Parasitic Nematodes – Antigens, Membranes and Genes, (ed. Kennedy, M. W.), pp. 140–169. London: Taylor and Francis.Google Scholar
Smith, H. V., Quinn, R., Kusel, J. R. & Girdwood, R. W. A. (1981). The effect of temperature and antimetabolites on antibody binding to the outer surface of the second stage Toxocara canis larvae. Molecular and Biochemical Parasitology 4, 183–93.CrossRefGoogle Scholar
Smith, K. (1970). Electron-microscopical observations on the body wall of the third-stage larva of Haemonchus placei. Parasitology 60, 411–16.CrossRefGoogle ScholarPubMed
Soliman, K. N. (1953). Migration route of Dictyocaulus viviparus and D. filaria infective larvae to the lungs. Journal of Comparative Pathology 63, 75–83.Google Scholar
Sommerville, R. I. (1954). The second ecdysis of infective nematode larvae. Nature, London 174, 751.Google Scholar
Sulston, J. & Hodgkin, J. (1988). Methods. In The Nematode Caenorhabiditis elegans. (ed. Wood, W. B.), pp. 598–600. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory.Google Scholar
Urquhart, G. M. (1985). Field experience with the bovine lungworm vaccine. Developments in Biological Standardisation 62, 109–112.Google ScholarPubMed
Wharton, D. A. (1986). The structure of the cuticle and sheath of the infective juvenile of Trichostrongylus colubriformis. Zeitschrift für Parasitenkunde 72, 779–87.CrossRefGoogle ScholarPubMed