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Besnoitia besnoiti and Toxoplasma gondii: two apicomplexan strategies to manipulate the host cell centrosome and Golgi apparatus

Published online by Cambridge University Press:  03 June 2014

RITA CARDOSO ,
SOFIA NOLASCO ,
JOÃO GONÇALVES ,
HELDER C. CORTES ,
ALEXANDRE LEITÃO  and
HELENA SOARES
RITA CARDOSO
Affiliation:
Instituto de Investigação Científica Tropical, CVZ, CIISA Faculdade de Medicina Veterinária, Universidade de Lisboa, Av. Universidade Técnica, 1300-447 Lisboa, Portugal Centro de Investigação Interdisciplinar em Sanidade Animal, FMV, Universidade de Lisboa, Avenida da Universidade Técnica, 1300-477 Lisboa, Portugal Instituto Gulbenkian de Ciência, 2781-901 Oeiras, Portugal
SOFIA NOLASCO
Affiliation:
Centro de Investigação Interdisciplinar em Sanidade Animal, FMV, Universidade de Lisboa, Avenida da Universidade Técnica, 1300-477 Lisboa, Portugal Instituto Gulbenkian de Ciência, 2781-901 Oeiras, Portugal Escola Superior de Tecnologia da Saúde de Lisboa, 1990-096 Lisboa, Portugal
JOÃO GONÇALVES
Affiliation:
Instituto Gulbenkian de Ciência, 2781-901 Oeiras, Portugal Centro de Química e Bioquímica, Departamento de Química e Bioquímica, Faculdade de Ciências, Universidade de Lisboa, 1749-016 Lisboa, Portugal
HELDER C. CORTES
Affiliation:
Laboratório de Parasitologia Victor Caeiro, ICAAM – Instituto de Ciências Agrárias e Ambientais Mediterrânicas – Universidade de Évora – Núcleo da Mitra, Ap. 94, 7002-554 Évora, Portugal
ALEXANDRE LEITÃO*
Affiliation:
Instituto de Investigação Científica Tropical, CVZ, CIISA Faculdade de Medicina Veterinária, Universidade de Lisboa, Av. Universidade Técnica, 1300-447 Lisboa, Portugal Centro de Investigação Interdisciplinar em Sanidade Animal, FMV, Universidade de Lisboa, Avenida da Universidade Técnica, 1300-477 Lisboa, Portugal
HELENA SOARES*
Affiliation:
Instituto Gulbenkian de Ciência, 2781-901 Oeiras, Portugal Escola Superior de Tecnologia da Saúde de Lisboa, 1990-096 Lisboa, Portugal Centro de Química e Bioquímica, Departamento de Química e Bioquímica, Faculdade de Ciências, Universidade de Lisboa, 1749-016 Lisboa, Portugal
*
*Corresponding author: IICT, CVZ, CIISA Faculdade de Medicina Veterinária, Universidade de Lisboa, Av. Universidade Técnica, 1300-447 Lisboa, Portugal. E-mail: alexandre@fmv.ulisboa.pt
*Corresponding author: Centro de Química e Bioquímica, Departamento de Química e Bioquímica, Faculdade de Ciências, Universidade de Lisboa, 1749-016 Lisboa, Portugal. E-mail: mhsoares@fc.ul.pt
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Summary

Besnoitia besnoiti and Toxoplasma gondii are two closely related parasites that interact with the host cell microtubule cytoskeleton during host cell invasion. Here we studied the relationship between the ability of these parasites to invade and to recruit the host cell centrosome and the Golgi apparatus. We observed that T. gondii recruits the host cell centrosome towards the parasitophorous vacuole (PV), whereas B. besnoiti does not. Notably, both parasites recruit the host Golgi apparatus to the PV but its organization is affected in different ways. We also investigated the impact of depleting and over-expressing the host centrosomal protein TBCCD1, involved in centrosome positioning and Golgi apparatus integrity, on the ability of these parasites to invade and replicate. Toxoplasma gondii replication rate decreases in cells over-expressing TBCCD1 but not in TBCCD1-depleted cells; while for B. besnoiti no differences were found. However, B. besnoiti promotes a reorganization of the Golgi ribbon previously fragmented by TBCCD1 depletion. These results suggest that successful establishment of PVs in the host cell requires modulation of the Golgi apparatus which probably involves modifications in microtubule cytoskeleton organization and dynamics. These differences in how T. gondii and B. besnoiti interact with their host cells may indicate different evolutionary paths.

Keywords

Besnoitia besnoitiToxoplasma gondiimicrotubule cytoskeleletonGolgi apparatuscentrosomeparasite-host interaction
Type
Special Issue Article
Information
Parasitology , Volume 141 , Special Issue 11: ApiCOWplexa 2013 – 2nd International Meeting on Apicomplexan Parasites in Farm Animals , September 2014 , pp. 1436 - 1454
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Copyright
Copyright © Cambridge University Press 2014 

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