Hostname: page-component-5c6d5d7d68-vt8vv Total loading time: 0.001 Render date: 2024-08-11T07:09:11.288Z Has data issue: false hasContentIssue false
  • English
  • Français

Characterization of Trypanosoma brucei isolated from lymph nodes of rats

Published online by Cambridge University Press:  06 April 2009

M. Tanner ,
L. Jenni ,
H. Hecker  and
R. Brun
M. Tanner
Affiliation:
Swiss Tropical Institute, Socinstrasse 57, CH-4051 Basel, Switzerland
L. Jenni
Affiliation:
Swiss Tropical Institute, Socinstrasse 57, CH-4051 Basel, Switzerland
H. Hecker
Affiliation:
Swiss Tropical Institute, Socinstrasse 57, CH-4051 Basel, Switzerland
R. Brun
Affiliation:
Swiss Tropical Institute, Socinstrasse 57, CH-4051 Basel, Switzerland
Get access Rights & Permissions [Opens in a new window]

Summary

Forms of Trypanosoma brucei isolated from lymph nodes of cyclically or intravenously infected rats, at different stages of infection, were morphologically compared with the corresponding bloodstream forms. Unlike the pleomorphic bloodstream trypanosome population, the lymph node trypanosome population was essentially monomorphic and resembled the slender forms of the bloodstream. Values for the morphometric parameters indicated a position between the slender and the stumpy forms. The number of trypanosomes/ml of blood showed the well-known periodic fluctuations while the number of trypanosomes/g of lymph node remained fairly constant. Serology revealed that lymph node trypanosome populations differed antigenically from the trypanosome populations isolated simultaneously from the bloodstream.

Type
Research Article
Information
Parasitology , Volume 80 , Issue 2 , April 1980 , pp. 383 - 391
Copyright
Copyright © Cambridge University Press 1980

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Baker, J. R. (1979). Documentation of primary isolates of stocks of Trypanosoma brucei subspecies from Tanzania and Ethiopia. Transactions of the Royal Society of Tropical Medicine and Hygiene 73, 123.CrossRefGoogle ScholarPubMed
Balber, A. E. (1972). Trypanosoma brucei: fluxes of the morphological variants in intact and X-irradiated mice. Experimental Parasitology 31, 307–19.CrossRefGoogle ScholarPubMed
Boehringer, S. & Hecker, H. (1974). Quantitative ultrastructural differences between strains of the Trypanosoma brucei subgroup during transformation in blood. Journal of Protozoology 21, 694–8.CrossRefGoogle Scholar
Boehringer, S. & Hecker, H. (1975). Quantitative ultrastructural investigations of the life cycle of Trypanosoma brucei. A morphometric analysis. Journal of Protozoology 22, 463–7.Google Scholar
Brown, R. C., Evans, D. A. & Vickerman, K. (1973). Changes in the oxidative metabolism and ultrastructural differentiation of the mitochondrion in Trypanosoma brucei. International Journal for Parasitology 3, 691–704.CrossRefGoogle ScholarPubMed
Brun, R. & Jenni, L. (1977). A new semi-defined medium for Trypanosoma brucei sspp. Acta Tropica 34, 21–33.Google ScholarPubMed
Geigy, R., Jenni, L., Kauffmann, M., Onyango, R. J. & Weiss, N. (1975). Identification of T. brucei subgroup strains isolated from game. Acta Tropica 32, 190–205.Google ScholarPubMed
Goodwin, L. G. (1970). The pathology of African trypanosomiasis. Transactions of the Royal Society of Tropical Medicine and Hygiene 64, 797–817.Google Scholar
Hecker, H., Burri, P. H., Steiger, R. & Geigy, R. (1972). Morphometric data on the ultrastructure of the pleomorphic bloodfonns of Trypanosoma brucei, Plimmer and Bradford, 1899. Acta Tropica 29, 182–98.Google ScholarPubMed
Hecker, H., Burri, P. H. & Boehringer, S. (1973). Quantitative ultrastructural differences in the mitochondrion of pleomorphic bloodforms of Trypanosoma brucei. Experientia 29, 901–3.CrossRefGoogle ScholarPubMed
Jenni, L. (1977). Comparison of antigenic types of Trypanosoma (T.) brucei strains transmitted by Glossina m. morsitans. Acta Tropica 34, 35–41.Google Scholar
Le Ray, D., Barry, J. D., Easton, C. & Vickerman, K. (1977). First tsetse fly transmission of the ‘AnTat’ serodeme of Trypanosoma brucei. Annales de la Société belge de Médicine tropicale 57, 369–81.Google ScholarPubMed
Le Ray, D., Barry, J. D. & Vickerman, K. (1978). Antigenic heterogeneity of metacyclic forms of Trypanosoma brucei. Nature, London 273, 300–2.CrossRefGoogle ScholarPubMed
Losos, G. & Ikede, B. O. (1972). Review of pathology of diseases in domestic and laboratory animals caused by Trypanosoma congolense, T. vivax, T. brucei, T. rhodesiense and T. gambiense. Veterinary Pathology 9, 1–71.Google Scholar
Nantulya, V. M. & Doyle, J. J. (1977). Stabilization and preservation of the antigenic specificity of Trypanosoma (Trypanozoon) brucei variant-specific surface antigen by mild fixation techniques. Acta Tropica 34, 313–20.Google ScholarPubMed
Ormerod, W. E. & Venkatesan, S. (1971). The occult visceral phase of mammalian trypanosomes with special reference to the life cycle of Trypanosoma (Trypanozoon) brucei. Transactions of the Royal Society of Tropical Medicine and Hygiene 65, 722–35.Google Scholar
Schuberg, A. & Boeing, W. (1913). Ueber den Weg der Infektion bei Trypanosomen- und Spirochätenerkrankungen. Deutsche Medizinische Wochenschrift 19, 877–9.Google Scholar
Seed, J. R. (1978). Competition among serologically different clones of Trypanosoma brucei gambiense in vivo. Journal of Protozoology 25, 526–9.CrossRefGoogle ScholarPubMed
Seed, J. R. & Effron, H. G. (1973). Simultaneous presence of different antigenic populations of Trypanosoma brucei gambiense in Microtus montanus. Parositology 66, 269–78.CrossRefGoogle ScholarPubMed
Soltys, M. A., Woo, P. & Gillick, A. C. (1969). A preliminary note on the separation and infectivity of tissue forms of Trypanosoma brucei. Transactions of the Royal Society of Tropical Medicine and Hygiene 63, 495–6.Google Scholar
Ssenyonga, G. S. Z. & Adam, K. M. (1975). The number and morphology of trypanosomes in the blood and lymph of rats infected with Trypanosoma brucei and T congolense. Parsitology 70, 255–61.Google Scholar
Van Meirvenne, N., Janssens, P. G. & Magnus, E. (1975). Antigenic variation in syringe passaged populations of Trypanosoma (Trypanozoon) brucei. I. Rationalization of the experimental approach. Annales de la Société belge de Médicine tropicale 55, 1–23.Google Scholar
Walker, P. J. (1970). Techniques for manipulation and maintenance of trypanosome strains in the laboratory. In The African Trypanosomiasis (ed. Mulligan, H. W.), pp. 89–96. London: George Allan and Unwin.Google Scholar