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Cloning and functional expression of a Shaker-related voltage-gated potassium channel gene from Schistosoma mansoni (Trematoda: Digenea)

Published online by Cambridge University Press:  06 April 2009

E. Kim ,
T. A. Day ,
J. L. Bennett  and
R. A. Pax
E. Kim
Affiliation:
Department of Pharmacology and Toxicology and Department of Zoology, Michigan State University, East Lansing, Michigan 48824, USA
T. A. Day
Affiliation:
Department of Pharmacology and Toxicology and Department of Zoology, Michigan State University, East Lansing, Michigan 48824, USA
J. L. Bennett
Affiliation:
Department of Pharmacology and Toxicology and Department of Zoology, Michigan State University, East Lansing, Michigan 48824, USA
R. A. Pax
Affiliation:
Department of Pharmacology and Toxicology and Department of Zoology, Michigan State University, East Lansing, Michigan 48824, USA
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Summary

We have isolated a cDNA (SKvl. 1) encoding a Shaker-related K+ channel from an adult cDNA library of the human parasitic trematode Schistosoma mansoni. The deduced amino acid sequence (512 aa, 56·5 kDa) contains 6 putative membrane-spanning domains (S1–S6) and a pore-forming domain (H5). SKv1.1 is grouped in the Shaker family, but forms a unique branch within this family, on the basis of dendrogram analysis. SKv1.1 shows significant sequence identity with most other Shaker channels, with 64—74% identity in the core region (S1–S6). It has the highest sequence identity with the K+ channel (Ak01a) from Aplysia. Northern blot analysis detected a single primary transcript of 2·8 kb. Southern blot analysis indicated that SKv1.1 is present as a single copy in the genomic DNA of S. mansoni. Expression of SKv1. 1 in Xenopus oocytes produced a rapidly activating and inactivating outward K+ current which is highly sensitive to 4-aminopyridine, but is insensitive to tetraethylammonium, mast cell degranulating peptide, dendrotoxin and charybdo-toxin. The presence of a Shaker homologue in Schistosoma suggests that Sh subfamilies may exist in other lower invertebrates as well as platyhelminths.

Keywords

Schistosoma mansoniplatyhelminthtrematodepotassium channelShakercloningexpressionXenopusoocyte
Type
Research Article
Information
Parasitology , Volume 110 , Issue 2 , February 1995 , pp. 171 - 180
Copyright
Copyright © Cambridge University Press 1995

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