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Detection and molecular identification of Hepatozoon canis and Babesia vogeli from domestic dogs in Palestine

Published online by Cambridge University Press:  12 December 2016

KIFAYA AZMI ,
AMER AL-JAWABREH ,
ABEDELMAJEED NASEREDDIN ,
AHMAD ABDELKADER ,
TAHER ZAID ,
SUHEIR EREQAT ,
SAMER S. SAWALHA ,
GAD BANETH  and
ZIAD ABDEEN
KIFAYA AZMI*
Affiliation:
Biochemistry and Molecular Biology Department, Faculty of Medicine, Al-Quds University, Abu Deis, The West Bank, Palestine Faculty of Medicine, Al-Quds Nutrition and Health Research Institute, Al-Quds University, Abu-Deis, P.O. Box 20760, The West Bank, Palestine Al-Quds Public Health Society, Jerusalem, Palestine
AMER AL-JAWABREH
Affiliation:
Al-Quds Public Health Society, Jerusalem, Palestine
ABEDELMAJEED NASEREDDIN
Affiliation:
Al-Quds Public Health Society, Jerusalem, Palestine
AHMAD ABDELKADER
Affiliation:
Faculty of Medicine, Al-Quds Nutrition and Health Research Institute, Al-Quds University, Abu-Deis, P.O. Box 20760, The West Bank, Palestine Al-Quds Public Health Society, Jerusalem, Palestine
TAHER ZAID
Affiliation:
Faculty of Medicine, Al-Quds Nutrition and Health Research Institute, Al-Quds University, Abu-Deis, P.O. Box 20760, The West Bank, Palestine Al-Quds Public Health Society, Jerusalem, Palestine
SUHEIR EREQAT
Affiliation:
Biochemistry and Molecular Biology Department, Faculty of Medicine, Al-Quds University, Abu Deis, The West Bank, Palestine Faculty of Medicine, Al-Quds Nutrition and Health Research Institute, Al-Quds University, Abu-Deis, P.O. Box 20760, The West Bank, Palestine Al-Quds Public Health Society, Jerusalem, Palestine
SAMER S. SAWALHA
Affiliation:
Department of Primary Health Care, Palestinian Ministry of Health, Ramallah, Palestine
GAD BANETH
Affiliation:
Koret School of Veterinary Medicine, Hebrew University, Rehovot, Israel
ZIAD ABDEEN
Affiliation:
Faculty of Medicine, Al-Quds Nutrition and Health Research Institute, Al-Quds University, Abu-Deis, P.O. Box 20760, The West Bank, Palestine Al-Quds Public Health Society, Jerusalem, Palestine
*
*Corresponding author. Biochemistry and Molecular Biology Department, Faculty of Medicine, Al-Quds University, P.O. Box: 20002, The West Bank, Palestine. E-mail: kifaya_alkam@yahoo.com, ksuleiman@staff.alquds.edu
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Summary

Dogs serve as hosts for a great number of parasites, which may affect their health and wellbeing. This study aimed to observe tick borne pathogens in dogs from Palestine including Hepatozoon canis and Babesia species. The prevalence of both H. canis and Babesia species infections in apparently healthy dogs, from ten districts of the West Bank was surveyed. DNA was extracted from blood samples obtained from dogs (n = 362) and ticks (n = 213) collected from dogs (n = 77). A primer set that amplifies a partial sequence of the Babesia and Hepatozoon 18S rRNA gene was used for PCR and the DNA sequences of the PCR products of all samples were determined. Twenty-nine (8·0%) of the dogs were found infected including 20 with H. canis (5·5%), seven with Babesia vogeli (1·9%) and two with undefined Babesia spp. (0·6%). Twelve Rhipicephalus sanguineus s.l ticks were pathogen-positive, including ten with H. canis (4·7%), one with B. vogeli (0·5%), and one with Hepatozoon felis (0·5%). The results indicated that a wide range of tick borne pathogens is circulating in the canine population in the surveyed region. This study is the first report on the prevalence of H. canis, B. vogeli and Babesia spp. in dogs in Palestine and its results will assist in the management of diseases associated with these blood parasites.

Keywords

canine babesiosisBabesia vogeliHepatozoan canis
Type
Research Article
Information
Parasitology , Volume 144 , Issue 5 , April 2017 , pp. 613 - 621
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Copyright
Copyright © Cambridge University Press 2016 

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