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The development of a standard method for rapid ecdysis of infective Haemonchus contortus cayugensis larvae

Published online by Cambridge University Press:  06 April 2009

J. Owen D. Slocombe  and
J. H. Whitlock
J. Owen D. Slocombe
Affiliation:
Department of Pathology, New York State Veterinary College, Cornell University, Ithaca, N. Y.
J. H. Whitlock
Affiliation:
Department of Pathology, New York State Veterinary College, Cornell University, Ithaca, N. Y.
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Extract

Infective Haemonchus contortus cayugensis larvae were rapidly exsheathed (20 min) using 0·02 m-Na2B4O7. 10H2O, a gas mixture of 40 % CO2–60 % N2, and subsequent aeration with compressed air as reported previously. Several variables in this system were examined. The optimum concentration of sodium tetraborate was found to be 0·02 m. The exsheathment rate decreased with increasing molarities of the salt over the range 0·02–0·08 m. Exsheathment rates were low when the larvae received the full stimulus to exsheath for only 5 and 10 min before aeration. The larvae required at least 15 min of the full stimulus before aeration to ensure high exsheathment rates. The rapid method of ecdysis caused no abnormalities in or death of larvae, and all the larvae at the end of the incubation period were very motile and appeared normal. The exsheathment rate was consistently high as the larval concentration was increased from 2 ×103 to 2×106/10 ml of 0·02 m-Na2B4O7. 10H2O.

Type
Research Article
Information
Parasitology , Volume 61 , Issue 2 , October 1970 , pp. 273 - 277
Copyright
Copyright © Cambridge University Press 1970

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References

REFERENCES

Davey, K. G. & Sau Pheng Kan. (1968). Molting in a parasitic nematode, Phocanema decipens. IV. Ecdysis and its control. Canadian Journal of Zoology 46, 893–8.CrossRefGoogle Scholar
Lapage, G. (1935). The second ecdysis of infective nematode larvae. Parasitology 27, 186–206.CrossRefGoogle Scholar
Ozerol, N. H. & Silverman, P. H. (1969). Partial characterization of Haemonchus contortus exsheathing fluid. Journal of Parasitology 55, 79–87.Google Scholar
Rogers, W. P. (1963). Physiology of infection with nematodes. Some effects of the host stimulus on infective stages. Annals of the New York Academy of Sciences 113 (Art. 1) 208–16.CrossRefGoogle ScholarPubMed
Rogers, W. P. (1965). The role of leucine aminopeptidase in the moulting of nematode parasites. Comparative Biochemistry and Physiology 14, 311–21.Google Scholar
Rogers, W. P. & Sommerville, R. I. (1960). The physiology of the second ecdysis of parasitic nematodes. Parasitology 50, 329–48.CrossRefGoogle ScholarPubMed
Silverman, P. H. & Podger, K. R. (1964). In vitro exsheathment of some nematode larvae. Experimental Parasitology 15, 314–24.CrossRefGoogle Scholar
Slocombe, J. O. D. (1969). Analysis of parameters in exsheathment of infective larvae of Haemonchus contortus cayugensis. Das & Whitlock, 1960. Ph.D. Thesis, Cornell University.Google Scholar
Slocombe, J. O. D. & Whitlock, J. H. (1969). Rapid ecdysis of infective Haemonchus contortus cayugensis larvae. Journal of Parasitology 55, 1102–3.CrossRefGoogle Scholar
Slocombe, J. O. D. & Whitlock, J. H. (1970 a). Effects of heat and Lugol's iodine on the evaluation of ecdysis of infective Haemonchus contortus cayugensis larvae. Transactions of the American Microscopical Society 89, 71–4.CrossRefGoogle ScholarPubMed
Slocombe, J. O. D. & Whitlock, J. H. (1970 b). The inhibitory effect of CO2 on the ecdysis of infective Haemonchus contortus cayugensis larvae. Parasitology 61, 253–8.Google Scholar
Slocombe, J. O. D. & Whitlock, J. H. (1970 c). The influence of pCO2 and the rate of shaking on the ecdysis of infective Haemonchus contortus cayugensis larvae. Parasitology 61, 259–65.CrossRefGoogle ScholarPubMed
Slocombe, J. O. D. & Whitlock, J. H. (1970 d). The effects of larval age and cold storage on the ecdysis of infective Haemonchus contortus cayugensis larvae. Parasitology 61, 267–71.CrossRefGoogle ScholarPubMed