Hostname: page-component-77c89778f8-sh8wx Total loading time: 0 Render date: 2024-07-20T18:36:24.364Z Has data issue: false hasContentIssue false
  • English
  • Français

Eimeria species: studies using rRNA and rDNA probes

Published online by Cambridge University Press:  06 April 2009

J. Ellis  and
J. Bumstead
J. Ellis
Affiliation:
Department of Parasitology, AFRC Institute for Animal Health, Houghton Laboratory, Houghton, Huntingdon, Cambs PE17 2DA
J. Bumstead
Affiliation:
Department of Parasitology, AFRC Institute for Animal Health, Houghton Laboratory, Houghton, Huntingdon, Cambs PE17 2DA
Get access Rights & Permissions [Opens in a new window]

Summary

rRNA and a heterologous cloned rDNA probe have been used to detect the rRNA genes of Eimeria species which infe the chicken, and has allowed the isolation and preliminary characterization of cloned rDNA sequences from a genomic DNA library of Eimeria tenella. It is demonstrated that rRNA and rDNA probes can be used to identify individual Eimeria species by the restriction fragment patterns detected after Southern hybridization. In addition, studies have shown that the large and small subunit rRNAs are expressed throughout sporulation.

Keywords

Eimeria spp.rRNArDNADNA probes
Type
Research Article
Information
Parasitology , Volume 101 , Issue 1 , August 1990 , pp. 1 - 6
Copyright
Copyright © Cambridge University Press 1990

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Arnot, D. E. & Barker, D. C. (1981). Biochemical identification of cutaneous leishmaneas by analysis of kinetoplast DNA. II. Sequence homologies in Leishmania kDNA. Molecular and Biochemical Parasitology 3, 47–56.CrossRefGoogle ScholarPubMed
Bantle, J. A., Maxwell, I. H. & Hahn, W. E. (1976). Specificity of oligo (dT) cellulose chromatography in the isolation of polyadenylated RNA. Analytical Biochemistry 72, 413–27.CrossRefGoogle ScholarPubMed
Brothers, V. M., Kuhn, I., Paul, L. S., Gabe, J. D., Andrews, W. H., Sias, S. R., Mccamen, M. T., Dragon, E. A. & Files, J. G. (1988). Characterisation of a surface antigen of Eimeria tenella sporozoites and synthesis from a cloned cDNA in Escherichia coli. Molecular and Biochemical Parasitology 28, 235–48.CrossRefGoogle ScholarPubMed
Carle, G. R., Frank, M. & Olson, M. V. (1986). Electrophoretic separations of large DNA molecules by periodic inversion of the electric field. Science 232, 65–8.CrossRefGoogle ScholarPubMed
Chapman, H. D. (1978). Studies on the excystation of different species of Eimeria in vitro. Zeitschrift für Parasitenkunde 56, 115–21.CrossRefGoogle ScholarPubMed
Chirgwin, J. M., Przybyla, A. E., Macdonald, R. J. & Rutter, W. J. (1979). Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry 18, 5294–9.CrossRefGoogle ScholarPubMed
Dame, J. B. & McCutchan, T. F. (1983). The four ribosomal DNA units of the malaria parasite Plasmodium berghei. Journal of Biological Chemistry, 258, 6984–90.CrossRefGoogle ScholarPubMed
Frischauf, A. M., Lehrach, H., Poustka, A. & Murray, N. (1983). Lambda replacement vectors carrying polylinker sequences. Journal of Molecular Biology 170, 827–42.CrossRefGoogle ScholarPubMed
Hasegawa, M., Iida, Y., Yano, T., Takaiwa, F. & Iwabuchi, M. (1985). Phylogenetic relationships among eukaryotic kingdoms inferred from ribosomal RNA sequences. Journal of Molecular Evolution 22, 32–8.CrossRefGoogle ScholarPubMed
Hull, R. & Al-hakin, X. (1988). Nucleic acid hybridisation in plant virus diagnosis and characterisation. Trends in Biotechnology 6, 213–18.CrossRefGoogle Scholar
Jenkins, M. C., Lillehoj, H. S. & Dame, J. B. (1988). Eimeria acervulina: DNA cloning and characterisation of recombinant sporozoite and merozoite antigens. Experimental Parasitology 66, 96–107.CrossRefGoogle ScholarPubMed
Johnson, A. M., Illana, S., Dubey, J. P. & Dame, J. B. (1987). Toxoplasma gondii and Hammondia hammondi: DNA comparison using cloned rRNA gene probes. Experimental Parasitology 63, 272–8.CrossRefGoogle ScholarPubMed
Langsley, G., Hyde, J. E., Goman, M. & Scaife, J. G. (1983). Cloning and characterisation of the rRNA genes from the human malaria parasite Plasmodium falciparum. Nucleic Acids Research 11, 8703–17.CrossRefGoogle ScholarPubMed
Long, E. O. & Dawid, I. B. (1980). Repeated genes in eukaryotes. Annual Review of Biochemistry 49, 727–64.CrossRefGoogle ScholarPubMed
Long, P. L., Joyner, L. P., Millard, B. J. & Norton, C. C. (1976). A guide to laboratory techniques used in the study and diagnosis of avian coccidiosis. Folia Vet Lat 6, 201–17.Google Scholar
Maniatis, T., Fritsch, E. F. & Sambrook, J. (1982). Molecular Cloning, A Laboratory Manual. Cold Spring Harbor Laboratory.Google Scholar
McCutchan, T. F., de la Cruz, V. F., Lal, A. A., Gunderson, J. H., Elwood, H. J. & Sogin, M. L. (1988). Primary sequences of two small subunit ribosomal RNA genes from Plasmodium falciparum. Molecular and Biochemical Parasitology 28, 63–8.CrossRefGoogle ScholarPubMed
Ramirez, J. L. & Guevara, P. (1987). The ribosomal gene spacer as a tool for the taxonomy of Leishmania. Molecular and Biochemical Parasitology 22, 177–83.CrossRefGoogle ScholarPubMed
Rigby, P. W. J., Dieckman, M., Rhodes, C. & Berg, P. (1977). Labelling DNA to high specific activity in vitro by nick-translation with DNA polymerase I. Journal of Molecular Biology 113, 237–51.CrossRefGoogle ScholarPubMed
St John, T. P. & Davis, R. W. (1979). Isolation of galactose-inducible DNA sequences from Saccharomyces cerevisiae by differential plaque filter hybridisation. Cell 16, 443–52.CrossRefGoogle Scholar
Southern, E. (1975). Detection of specific sequences among DNA fragments separated by gel electrophoresis. Journal of Molecular Biology 98, 503–17.CrossRefGoogle ScholarPubMed
Tyzzer, E. E., Theiler, H. & Jones, E. E. (1932). Coccidiosis in gallinaceous birds: II A comparative study of species of Eimeria of the chicken. American Journal of Hygiene 15, 319–93.Google Scholar
Unnasch, T. R., McLafferty, M. & Wirth, D. F. (1985). The primary structure of the rRNA insertions of Plasmodium lophurae. Molecular and Biochemical Parasitology 16, 149–61.CrossRefGoogle ScholarPubMed