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Filtration studies on Nicotiana virus 11

Published online by Cambridge University Press:  06 April 2009

Kenneth M. Smith  and
W. D. MacClement
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Extract

An account is given of ultrafiltration studies with Nicotiana virus 11 (tobacco necrosis virus). It is shown that though the filtration end-point is about 40 mμ, there is invariably a sharp drop in the local lesion counts between the membrane pore sizes 250 and 125 mμ. This phenomenon shows itself as a “bench” or shelf with an upward trend in the filtration curve (see Text-fig. 1, curve A). A number of possible explanations are given. The fact that increasing dilution raises the height of the bench leads to the deduction that large particles are breaking up into smaller ones, the larger particles being aggregates of the smaller. Experiments on resuspension of virus inside the filter and fractional filtration support the same conclusion.

From the filtration end-point the particle size of the virus is computed to be between 13 and 20 mμ.

Type
Research Article
Information
Parasitology , Volume 32 , Issue 3 , August 1940 , pp. 320 - 332
Copyright
Copyright © Cambridge University Press 1940

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References

REFERENCES

Bald, J. G. & Briggs, G. E. (1937). Aggregation of virus particles. Nature, Lorvd., 140, 111.CrossRefGoogle Scholar
Elford, W. J. (1931). A new series of graded collodion membranes. J. Path. Bact. 34, 505–21.CrossRefGoogle Scholar
Elford, W. J. (1933). The principles of ultrafiltration as applied in biological studies. Proc. roy. Soc. B, 112, 384–406.Google Scholar
Elford, W. J., Grabar, P. & Fischer, W. (1936). Ultrafiltration studies with normal horse serum. Biochem. J. 30, 92–9.CrossRefGoogle ScholarPubMed
Pirie, N. W., Smith, , Kenneth, M., Spooner, E. T. C. & MacClement, W. D. (1938). Purified preparations of tobacco necrosis virus. Parasitology, 30, 543–51.CrossRefGoogle Scholar
Smith, , Kenneth, M. & Bald, J. G. (1935). A necrotic virus disease affecting tobacco and other plants. Parasitology, 27, 231–45.CrossRefGoogle Scholar
Smith, , Kenneth, M. & MacClement, W. D. (1938). The ultrafiltration of plant viruses. Proc. roy. Soc. B, 125, 295–7.Google Scholar