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A freeze-fracture study of the cuticle of adult Nippostrongylus brasiliensis (Nematoda)

Published online by Cambridge University Press:  06 April 2009

D. L. Lee ,
K. A. Wright  and
R. R. Shivers
D. L. Lee
Affiliation:
Department of Pure and Applied Biology, University of Leeds, Leeds LS2 9JT, U.K.
K. A. Wright
Affiliation:
(Deceased) Department of Zoology, University of Toronto, Toronto, Ontario M5S 1A1, Canada
R. R. Shivers
Affiliation:
Department of Zoology, University of Western Ontario, London, Ontario N6A 3K7, Canada
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Summary

The surface of the cuticle of adult Nippostrongylus brasiliensis has been studied by means of the freeze-fracture technique and by transmission electron microscopy. Some of the surface coat appears to have been shed from the surface of the cuticle of adults fixed in situ in the intestine of its host and from the surface of individuals removed from the intestine and freeze-fractured. Freeze-fracturing the cuticle of individuals removed from the host has shown that this surface coat varies in thickness from 30 to 90 nm. The epicuticle is about 20 nm thick and cleaves readily to expose E- and P-faces. The P-face of the epicuticle possesses a small number of particles, similar to intra-membranous particles, whilst the E-face possesses a few, widely scattered depressions. Despite the presence of these particles the epicuticle is not considered to be a true membrane. Freeze-fracturing the remainder of the cuticle has confirmed its structure as described by conventional transmission electron microscopy. Clusters of particles on the P-face of the outer epidermal (hypodermal) membrane and corresponding depressions on the E-face of the membrane are thought to be associated with points of attachment of the cuticle to the epidermis (hypodermis). No differences in appearance of the cuticle and its surface layers were observed in individuals taken from 7-, 10-, 13- and 15-day infections.

Keywords

cuticleepicuticlefreeze-fractureintra-membranous particlesnematodeNippostrongylus brasiliensissurface coat
Type
Research Article
Information
Parasitology , Volume 107 , Issue 5 , December 1993 , pp. 545 - 552
Copyright
Copyright © Cambridge University Press 1993

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References

REFERENCES

Badley, J. E., Grieve, R. B., Rockey, J. H. & Glickman, L. T. (1987). Immune-mediated adherence of eosinophils to Toxocara canis infective larvae: the role of excretory-secretory antigens. Parasite Immunology 9, 133–43.Google Scholar
Bird, A. F. (1984). Nematoda. In Biology of the Integument (ed. Bereiter-Hohn, J., Matolsty, A. G. & Richards, K. S.), pp. 212–33. Berlin: Springer-Verlag.CrossRefGoogle Scholar
Bird, A. F. & Bird, J. (1991). The Structure of Nematodes, 2nd Edn. San Diego: Academic Press.Google Scholar
Blaxter, M. L., Page, A. P., Rudin, W. & Maizels, R. M. (1992). Nematode surface coats: actively evading immunity. Parasitology Today 8, 243–7.CrossRefGoogle ScholarPubMed
Fok, E., Mizinska-Boevska, Y. & Polyakova-Krusteva, O. (1991). Nippostrongylus brasiliensis — ultrastructure of the body wall. Parasitologia Hungarica 24, 81–7.Google Scholar
Kennedy, M. W. (1991). Parasitic Nematodes — Antigens, Membranes and Genes. London and Philadelphia: Taylor & Francis.Google Scholar
Kennedy, M. W., Foley, M., Kuo, Y.-M., Kusel, J. R. & Garland, P. B. (1987). Biophysical properties of the surface lipid of parasitic nematodes. Molecular and Biochemical Parasitology 22, 233–40.Google Scholar
Lee, D. L. (1965). The cuticle of adult Nippostrongylus brasiliensis. Parasitology 55, 173–81.Google Scholar
Lee, D. L. (1969). Changes in adult Nippostrongylus brasiliensis during the development of immunity to this nematode in rats. 1. Changes in ultrastructure. Parasitology 59, 29–39.Google Scholar
Lee, D. L. (1970). Moulting in nematodes: the formation of the adult cuticle during the final moult of Nippostrongylus brasiliensis. Tissue and Cell 2, 139–53.CrossRefGoogle ScholarPubMed
Lee, D. L. (1977). The nematode epidermis and collagenous cuticle, its formation and ecdysis. Symposia of the Zoological Society of London 39, 145–70.Google Scholar
Lee, D. L. & Atkinson, H. J. (1976). The Physiology of Nematodes, 2nd Edn. London: Macmillan.Google Scholar
Lee, D. L. & Nicholls, C. D. (1983). The use of plasma etching to reveal the internal structure of Nippostrongylus brasiliensis (Nematoda). Parasitology 86, 477–80.Google Scholar
Lee, D. L., Wright, K. A. & Shivers, R. R. (1984). A freeze-fracture study of the surface of the infective-stage larva of the nematode Trichinella. Tissue and Cell 16, 819–28.CrossRefGoogle ScholarPubMed
Lee, D. L., Wright, K. A. & Shivers, R. R. (1986). A freeze-fracture study of the body wall of adult, in utero larvae and infective-stage larvae of Trichinella (Nematoda). Tissue and Cell 18, 219–30.Google Scholar
Martinez-paloma, A. (1978). Ultrastructural characterization of the cuticle of Onchocerca volvulus microfilaria. Journal of Parasitology 64, 127–36.Google Scholar
Ogilvie, B. M. & Hockley, D. J. (1968). Effects of immunity on Nippostrongylus brasiliensis adult worms: reversible and irreversible changes in infectivity, reproduction and morphology. Journal of Parasitology 54, 1073–84.CrossRefGoogle ScholarPubMed
Philipp, M., Parkhouse, R. M. E. & Ogilvie, B. M. (1980). Changing proteins on the surface of a parasitic nematode. Nature, London 257, 538–40.CrossRefGoogle Scholar
Politz, S. M. & Philipp, M. (1992). Caenorhabditis elegans as a model for parasitic nematodes: a focus on the cuticle. Parasitology Today 8, 6–12.CrossRefGoogle Scholar
Preston-Meek, C. M. & Pritchard, D. I. (1991). Synthesis and replacement of nematode cuticle components. In Parasitic Nematodes — Antigens, Membranes and Genes (ed. Kennedy, M. W.), pp. 84–94. London and Philadelphia: Taylor & Francis.Google Scholar
Proudfoot, L., Kusel, J. R., Smith, H. V. & Kennedy, M. W. (1991). Biophysical properties of the nematode surface. In Parasitic Nematodes — Antigens, Membranes and Genes (ed. Kennedy, M. W.). London: Taylor & Francis.Google Scholar
Proudfoot, L., Kusel, J. R., Smith, H. V., Harnett, W., Worms, M. J. & Kennedy, M. W. (1993). Rapid changes in the surface of parasitic nematodes during transition from pre- to post-parasitic forms. Parasitology 107, 107–17.Google Scholar
Sharpe, M. J. & Lee, D. L. (1981). Observations on the structure and function of the haemoglobin from the cuticle of Nippostrongylus brasiliensis (Nematoda). Parasitology 83, 411–24.Google Scholar
Smith, H. V., Quinn, R., Kusel, J. R. & Girdwood, R. W. A. (1981). The effect of temperature and antimetabolites on antibody-binding to the outer surface of second-stageToxocara canis larvae. Molecular and Biochemical Parasitology 4, 183–93.CrossRefGoogle Scholar
Vetter, J. C. M. & Klaver-Wesseling, J. C. M. (1978). IgG antibody binding to the outer surface of infective larvae of Ancylostoma caninum. Zeitschrift für Parasitenkunde 58, 91–6.Google Scholar
Wright, K. A. (1987). The nematode's cuticle — its surface and the epidermis: function, homology, analogy — a current consensus. Journal of Parasitology 73, 1077–83.Google Scholar
Wright, K. A. & Hong, H. (1988). Characterization of the accessory layer of the cuticle of muscle larvae of Trichinella spiralis. Journal of Parasitology 74, 440–51.CrossRefGoogle ScholarPubMed
Wright, K. A., Lee, D. L. & Shivers, R. R. (1985). A freeze-fracture study of the digestive tract of the parasitic nematode Trichinella. Tissue and Cell 17, 189–98.Google Scholar