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Frequency of Toxoplasma gondii infection in sheep from a tropical zone of Mexico and temporal analysis of the humoral response changes

Published online by Cambridge University Press:  12 May 2008

H. CABALLERO-ORTEGA ,
H. QUIROZ-ROMERO ,
S. OLAZARÁN-JENKINS  and
D. CORREA
H. CABALLERO-ORTEGA
Affiliation:
Laboratorio de Inmunología Experimental, Instituto Nacional de Pediatría, Secretaría de Salud, México DF, México
H. QUIROZ-ROMERO
Affiliation:
Facultad de Medicina Veterinaria y Zootecnia-Universidad Nacional Autónoma de México, México DF, México
S. OLAZARÁN-JENKINS
Affiliation:
Centro Experimental Pecuario del Estado de Puebla (CIPEP)-INIFAP, Hueytamalco, Puebla, México
D. CORREA*
Affiliation:
Laboratorio de Inmunología Experimental, Instituto Nacional de Pediatría, Secretaría de Salud, México DF, México
*
*Corresponding author: Laboratorio de Inmunología Experimental, Torre de Investigación, 8o piso, Instituto Nacional de Pediatría, Insurgentes Sur 3700-C, México, D.F., C.P. 04530, México. Tel: +52 55 10 84 09 00. Ext. 1455 or 1458. Fax: +52 55 10 84 38 83. E-mail: mariadol@yahoo.com or mariadol@salud.gob.mx
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Summary

An indirect ELISA and an immunoblot were standardized to detect anti-Toxoplasma gondii antibodies in sheep, and were compared with a commercial ELISA as reference. Sensitivity was 92·0 and 96·0%, and specificity 88·0 and 75·0%, respectively. Then the serum samples of 103 sheep on a ranch located in the Eastern region of Mexico were analysed. A frequency ranging from 77 to 84% was observed, with a heterogeneous pattern among the animals by immunoblotting. Ten months later 56 sheep were sampled and tested again. Six animals became negative while 1 case was negative the first time and strongly positive 10 months later. Considering this to be a new case we calculated an incidence rate of 2·1% (CI95% 0·6–4·8%). IgG avidity ELISA was performed on 36 positive samples, 33 being of high-avidity at both times; slight increases in 2 samples and conservation of low-avidity in 1 sheep were also observed. Higher prevalence rates of toxoplasmosis in a moist warm compared to a cold atmosphere was attributed to the long viability of T. gondii oocysts. This may explain the high frequency of T. gondii in this region, which apparently has favourable climatic conditions for the transmission of this protozoan, besides the presence of both domestic and wild cats.

Keywords

frequencyimmunoassayMexicosheepToxoplasma gondii
Type
Original Articles
Information
Parasitology , Volume 135 , Issue 8 , July 2008 , pp. 897 - 902
Copyright
Copyright © 2008 Cambridge University Press

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References

REFERENCES

Blewett, D. A. and Watson, W. A. (1983). The epidemiology of ovine toxoplasmosis. II. Possible sources of infection in out-breaks of clinical disease. British Veterinary Journal 139, 546–555.CrossRefGoogle Scholar
Buxton, D. (1990). Ovine toxoplasmosis: a review. Journal of the Royal Society of Medicine 83, 509–511.CrossRefGoogle ScholarPubMed
Clementino, M. M., Souza, M. F. and Neto, V. F. (2007). Seroprevalence and Toxoplasma gondii-IgG avidity in sheep from Lajes, Brazil. Veterinary Parasitology 146, 199–203.CrossRefGoogle ScholarPubMed
Conraths, F. J. and Schares, G. (2006). Validation of molecular-diagnostic techniques in the parasitological laboratory. Veterinary Parasitology 136, 91–98.CrossRefGoogle ScholarPubMed
Cox, B. T., Reichel, M. P. and Griffiths, L. M. (1998). Serology of a Neospora abortion outbreak on a dairy farm in New Zealand: a case study. New Zealand Veterinary Journal 46, 28–31.CrossRefGoogle ScholarPubMed
Cruz-Vazquez, C., Garcia-Vazquez, Z., Rosario-Cruz, R. and Solorzano-Salgado, M. (1992). Ovine toxoplasmosis in Huitzilac, Morelos, Mexico. Preventive Veterinary Medicine 12, 27–33.CrossRefGoogle Scholar
De Lucas-Tron, J. and Arbiza-Aguirre, S. (2005). Situación y perspectivas de la producción de carne ovina. La Revista del Borrego (Méx.) 32, 1–7. (http://borrego.com.mx/).Google Scholar
Dubey, J. P. (2004). Toxoplasmosis – a waterborne zoonosis. Veterinary Parasitology 126, 57–72.CrossRefGoogle ScholarPubMed
Dubey, J. P. and Lindsay, D. S. (2006). Neosporosis, toxoplasmosis, and sarcocystosis in ruminants. Veterinary Clinical and Food Animal 22, 645–671.CrossRefGoogle ScholarPubMed
Dubey, J. P. and Welcome, F. L. (1988). Toxoplasma gondii-induced abortion in sheep. Journal of the American Veterinary Medical Association 193, 697–700.Google ScholarPubMed
Dumètre, A., Ajzenberg, D., Rozette, L., Mercier, A. and Darde, M. L. (2006). Toxoplasma gondii infection in sheep from Haute-Vienne, France: seroprevalence and isolate genotyping by microsatellite analysis. Veterinary Parasitology 42, 376–379.CrossRefGoogle Scholar
Fayer, R. (1981). Toxoplasmosis update and public health implications. Canadian Veterinary Journal 22, 344–352.Google ScholarPubMed
Figliuolo, L. P., Kasai, N., Ragozo, A. M., de Paula, V. S., Dias, R. A., Souza, S. L. and Gennari, S. M. (2004). Prevalence of anti-Toxoplasma gondii and anti-Neospora caninum antibodies in ovine from Sao Paulo State, Brazil. Veterinary Parasitology 123, 161–166.CrossRefGoogle ScholarPubMed
Figueroa-Castillo, J. A., Duarte-Rosas, V., Juárez-Acevedo, M., Luna-Pastén, H. and Correa, D. (2006). Prevalence of Toxoplasma gondii antibodies in rabbits (Oryctolagus cuniculus) from Mexico. Journal of Parasitology 92, 394–395.CrossRefGoogle ScholarPubMed
Fleck, D. G. (1972). The seroepidemiology of Toxoplasma infection in man. Proceedings of the Royal Society of Medicine 65, 1002.CrossRefGoogle ScholarPubMed
Gaffuri, A., Giacometti, M., Tranquillo, V. M., Magnino, S., Cordioli, P. and Lanfranchi, P. (2006). Serosurvey of roe deer, chamois and domestic sheep in the Central Italian Alps. Journal of Wildlife Diseases 42, 685–690.CrossRefGoogle ScholarPubMed
García-Vázquez, Z., Rosario-Cruz, R. and Solorzano-Salgado, M. (1990). Prevalence of antibodies against Toxoplasma gondii in sheep and goats in three states of Mexico. Preventive Veterinary Medicine 10, 25–29.CrossRefGoogle Scholar
Harkins, D., Clements, D. N., Maley, S., Marks, J., Wright, S., Esteban, I., Innes, E. A. and Buxton, D. (1998). Western blot analysis of the IgG responses of ruminants infected with Neospora caninum and with Toxoplasma gondii. Journal of Comparative Pathology 119, 45–55.CrossRefGoogle ScholarPubMed
Hartley, W. J. and Marshall, S. C. (1957). Toxoplasmosis as a cause of ovine perinatal mortality. New Zealand Veterinary Journal 5, 119–124.CrossRefGoogle Scholar
Hashemi-Fesharki, R. (1996). Seroprevalence of Toxoplasma gondii in cattle, sheep and goats in Iran. Veterinary Parasitology 61, 1–3.CrossRefGoogle ScholarPubMed
Hove, T., Lind, P. and Mukaratirwa, S. (2005). Seroprevalence of Toxoplasma gondii infection in goats and sheep in Zimbabwe. Onderstepoort Journal of Veterinary Research 72, 267–272.CrossRefGoogle ScholarPubMed
Klun, I., Djurkovic-Djakovic, O., Katic-Radivojevic, S. and Nikolic, A. (2006). Cross-sectional survey on Toxoplasma gondii infection in cattle, sheep and pigs in Serbia: seroprevalence and risk factors. Veterinary Parasitology 135, 121–131.CrossRefGoogle ScholarPubMed
Masala, G., Porcu, R., Daga, C., Denti, S., Canu, G., Patta, C. and Tola, S. (2007). Detection of pathogens in ovine and caprine abortion samples from Sardinia, Italy, by PCR. Journal of Veterinary Diagnostic Investigation 19, 96–98.CrossRefGoogle ScholarPubMed
Montoya, J. G. and Liesenfeld, O. (2004). Toxoplasmosis. Lancet 363, 1965–1976.CrossRefGoogle ScholarPubMed
Ortega-Mora, L. M., Troncoso, J. M., Rojo-Vázquez, F. A. and Gómez-Bautista, M. (1992). Cross-reactivity of polyclonal serum antibodies generated against Cryptosporidium parvum oocysts. Infection and Immunity 60, 3442–3445.CrossRefGoogle ScholarPubMed
Paré, J., Hietala, S. and Thurmond, M. (1995). An enzyme-linked immunosorbent assay (ELISA) for serological diagnosis of Neospora sp. infection in cattle. Journal of Veterinary Diagnostic Investigation 7, 352–359.CrossRefGoogle ScholarPubMed
Romanelli, P. R., Freire, R. L., Vidotto, O., Marana, E. R., Ogawa, L., De Paula, V. S. O., Garcia, J. L. and Navarro, I. T. (2007). Prevalence of Neospora caninum and Toxoplasma gondii in sheep and dogs from Guarapuava farms, Parana State, Brazil. Research in Veterinary Science 82, 202–207.CrossRefGoogle ScholarPubMed
Sawadogo, P., Hafid, J., Bellete, B., Sung, R. T., Chakdi, M., Flori, P., Raberin, H., Hamouni, I. B., Chait, A. and Dalal, A. (2005). Seroprevalence of T. gondii in sheep from Marrakech, Morocco. Veterinary Parasitology 130, 89–92.CrossRefGoogle ScholarPubMed
Sevgili, M., Babür, C., Nalbantoğlu, S., Karaş, G. and Vatansever, Z. (2005). Determination of seropositivity for Toxoplasma gondii in sheep in Şanlıurfa Province. Turkish Journal of Veterinary and Animal Science 29, 107–111.Google Scholar
Sharif, M., Gholami, S., Ziaei, H., Daryani, A., Laktarashi, B., Ziapour, S. P., Rafiei, A. and Vahedi, M. (2007). Seroprevalence of Toxoplasma gondii in cattle, sheep and goats slaughtered for food in Mazandaran province, Iran, during 2005. Veterinary Journal 174, 422–424.CrossRefGoogle ScholarPubMed
Suaréz-Aranda, F., Galisteo, A. J., Hiramoto, R. M., Cardoso, R. P., Meireles, L. R., Miguel, O. and Andrade, H. F. (2000). The prevalence and avidity of Toxoplasma gondii IgG antibodies in pigs from Brazil and Peru. Veterinary Parasitology 91, 23–32.CrossRefGoogle ScholarPubMed
Tenter, A. M., Heckeroth, A. R. and Weiss, L. M. (2000). Toxoplasma gondii: from animals to humans. International Journal for Parasitology 30, 1217–1258.CrossRefGoogle ScholarPubMed
van der Puije, W. N., Bosompem, K. M., Canacoo, E. A., Wastling, J. M. and Akanmori, B. D. (2000). The prevalence of anti-Toxoplasma gondii antibodies in Ghanaian sheep and goats. Acta Tropica 76, 21–26.CrossRefGoogle ScholarPubMed
Vela-Amieva, M., Cañedo-Solares, I., Gutiérrez-Castrellón, P., Pérez-Andrade, M., González-Contreras, C., Ortíz-Cortés, J., Ortega-Velázquez, V., Galván-Ramírez, M. L., Ruiz-García, M., Saltigeral-Simentel, P., Ordaz-Favila, J. C., Sánchez, C. and Correa, D. (2005). Short report: neonatal screening pilot study of Toxoplasma gondii congenital infection in Mexico. American Journal of Tropical Medicine and Hygiene 72, 142–144.CrossRefGoogle ScholarPubMed
Velasco-Castrejón, O., Salvatierra-Izaba, B., Valdespino, J. L., Sedano-Lara, A. M., Galindo-Virgen, S., Magos, C., Llausas, A., Tapia-Conyer, R., Gutiérrez, G. and Sepúlveda, J. (1992). Seroepidemiology of toxoplasmosis in Mexico. Salud Pública de México 34, 222–229.Google ScholarPubMed
Vesco, G., Buffolano, W., La Chiusa, S., Mancuso, G., Caracappa, S., Chianca, A., Villari, S., Curro, V., Liga, F. and Petersen, E. (2007). Toxoplasma gondii infections in sheep in Sicily, southern Italy. Veterinary Parasitology 146, 3–8.CrossRefGoogle ScholarPubMed
Wastling, J. M., Harkins, D. and Buxton, D. (1994). Western blot analysis of the IgG response of sheep vaccinated with S48 Toxoplasma gondii (Toxovax). Research in Veterinary Science 57, 384–386.CrossRefGoogle ScholarPubMed
Wastling, J. M., Harkins, D., Maley, S., Innes, E., Panton, W., Thomson, K. and Buxton, D. (1995). Kinetics of the local and systemic antibody response to primary and secondary infection with S48 Toxoplasma gondii in sheep. Journal of Comparative Pathology 112, 53–62.CrossRefGoogle ScholarPubMed