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Genomic divergence in the ecologically differentiated English freshwater and marine strains of Pomphorhynchus laevis (Acanthocephala: Palaeacanthocephala): a preliminary investigation

Published online by Cambridge University Press:  06 April 2009

M. A. Munro ,
A. Reid  and
P. J. Whitfield
M. A. Munro
Affiliation:
Parasitology and Pest Biology Research Group, King's College London, Campden Hill Road, London W8 7AH
A. Reid
Affiliation:
Department of Biology, Imperial College of Science, Technology and Medicine, Prince Consort Road, London SW7 2BB
P. J. Whitfield
Affiliation:
Parasitology and Pest Biology Research Group, King's College London, Campden Hill Road, London W8 7AH
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Summary

In and around the British Isles, three biologically specialized strains of the fish acanthocephalan Pomphorhynchus laevis have been recognized, each differing in geographical distribution and both final and intermediate host utilization. Six restriction endonuclease digests of the genomic DNA of the English freshwater and marine strains were compared by genomic cross-hybridization using the randomly sheared and radio-isotope labelled genomic DNA of freshwater P. laevis, and also by using a heterologous isotope labelled ribosomal DNA probe. Autoradiographs of hybridized Southern blots provided evidence for considerable homogeneity in the total repetitive DNA of the two strains as well as some preliminary evidence for possible restriction fragment length polymorphisms using EcoR I and Pvu II.

Keywords

AcanthocephalaPomphorhynchus laevisDNAhybridization patterns
Type
Research Article
Information
Parasitology , Volume 101 , Issue 3 , December 1990 , pp. 451 - 454
Copyright
Copyright © Cambridge University Press 1990

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References

REFERENCES

Amin, O. M. (1975). Variability in Acanthocephalus parksidei Amin, 1974 (Acanthocephala: Echinorhynchidae). Journal of Parasitology 61, 307–17.CrossRefGoogle ScholarPubMed
Amin, O. M. (1984). Variability and redescription of Acanthocephalus dirus (Acanthocephala: Echinorhynchidae) from freshwater fishes in North America. Proceedings of the Helminthological Society of Washington 51, 225–37.Google Scholar
Brown, A. F. (1987). Anatomic variability and secondary sexual characteristics in Pomphorhynchus laevis (Müller, 1776) Acanthocephala. Systematic Parasitology 9, 213–19.CrossRefGoogle Scholar
Chambers, A. E., Almond, N. M., Knight, M., Simpson, A. J. G. & Parkhouse, R. M. E. (1986). Repetitive DNA as a tool for the identification and comparison of nematode variants: application to Trichinella isolates. Molecular and Biochemical Parasitology 21, 113–20.CrossRefGoogle ScholarPubMed
De Jong, A. J., Barker, J., Roos, M. & Gommers, F. J. (1989). Repetitive DNA and hybridization patterns demonstrate extensive variability between the sibling species Globodera rostochiensis and G. pallida. Parasitology 99, 133–8.CrossRefGoogle Scholar
Denhardt, D. T. (1966). A membrane filter technique for the detection of complementary DNA. Biochemical and Biophysical Research Communications 23, 641–6.CrossRefGoogle ScholarPubMed
Files, J. G. & Hirsh, D. (1981). Ribosomal DNA of Caenorhabditis elegans. Journal of Molecular Biology 149, 223–40.CrossRefGoogle ScholarPubMed
Kennedy, C. R. (1984). The status of flounders, Platichthys flesus L., as hosts of Pomphorhynchus laevis (Müller) and its survival in marine conditions. Journal of Fish Biology 24, 135–49.CrossRefGoogle Scholar
Kennedy, C. R., Bates, R. M. & Brown, A. F. (1989). Discontinuous distributions of the fish acanthocephalans Pomphorhynchus laevis and Acanthocephalus anguillae in Britain and Ireland: an hypothesis. Journal of Fish Biology 34, 607–19.CrossRefGoogle Scholar
Kennedy, C. R., Broughton, P. F. & Hine, P. M. (1976). The sites occupied by the acanthocephalan Pomphorhynchus laevis in the alimentary canal of fish. Parasitology 72, 195–206.CrossRefGoogle ScholarPubMed
Kennedy, C. R., Broughton, P. F. & Hine, P. M. (1978). The status of brown and rainbow trout, Salmo trutta and S. gairdneri as hosts of the acanthocephalan, Pomphorhynchus laevis. Journal of Fish Biology 13, 265–75.CrossRefGoogle Scholar
Maniatis, T., Fritsch, E. F. & Sambrook, J. (1982). Molecular Cloning: a Laboratory Manual, pp. 468. New York: Cold Spring Harbour Laboratory.Google Scholar
Mcmanus, D. P. & Rishi, A. K. (1989). Genetic heterogeneity within Echinococcus granulosus: isolates from different hosts and geographical areas characterized with DNA probes. Parasitology 99, 17–29.CrossRefGoogle ScholarPubMed
Munro, M. A., Whitfield, P. J. & Diffley, R. (1989). Pomphorhynchus laevis (Müller) in the flounder Platichthys flesus L., in the tidal Thames: population structure, microhabitat utilization and reproductive status in the field and under conditions of controlled salinity. Journal of Fish Biology 35, 719–35.CrossRefGoogle Scholar
Rollinson, D., Walker, T. K. & Simpson, A. J. G. (1986). The application of recombinant DNA technology to problems of helminth identification. Parasitology 91 (Suppl.), S53–S71.CrossRefGoogle Scholar
Sinclair, J. H. & Brown, D. B. (1971). Retention of common nucleotide sequences in the ribosomal deoxyribonucleic acid of eukaryotes and some of their physical characteristics. Biochemistry 10, 2761–9.CrossRefGoogle ScholarPubMed
Southern, E. M. (1975). Detection of specific sequences among DNA fragments separated by gel electrophoresis. Journal of Molecular Biology 98, 503–17.CrossRefGoogle ScholarPubMed
van Eys, G. J. J. M., Schoone, G. J., Ligthart, G. S., Alvor, J., Evans, D. A. & Terpstra, W. J. (1989). Identification of ‘Old World’ Leishmania by DNA recombinant probes. Molecular and Biochemical Parasitology 34, 53–62.CrossRefGoogle ScholarPubMed