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The host-parasite interface of Cyathocotyle bushiensis Khan, 1962 (Trematoda: Strigeoidea)

III. Electron microscope observations on non-specific phosphatase activity

Published online by Cambridge University Press:  06 April 2009

David A. Erasmus
David A. Erasmus
Affiliation:
Department of Zoology, University College, Cardiff, Gt. Britain
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Extract

A combination of histochemical and electron microscope techniques have demonstrated, in Cyathocotyle bushiensis, alkaline phosphatase activity in the matrix of the tegument, in the distal and basal plasma membranes of the tegument, in the wall of the ducts extending from the adhesive organ gland cells and in the wall of the adhesive organ microvilli. Acid phosphatase activity was much stronger and was present in the tegument matrix and in the granular component of the secretion from the adhesive organ gland cells. Strong acid phosphatase activity was also present in the cisternae of the endoplasmic reticulum of the adhesive organ gland cells.

I am greatly indebted to Professor Brough for the excellent facilities available within this department. I also wish to thank Professor J. Sinclair (Department of Mining) for electron microscope facilities extended to me in the early stages of this investigation, and to Mr W. Henderson, Mr T. Davies and Miss M. Williams for their invaluable assistance. The purchase of the Huxley ultramicrotome, coating unit and an AEI EM 6 electron microscope was made possible by a grant from the Science Research Council.

Type
Research Article
Information
Parasitology , Volume 58 , Issue 2 , May 1968 , pp. 371 - 375
Copyright
Copyright © Cambridge University Press 1968

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References

REFERENCES

Erasmus, D. A. (1966). Electron microscope and histochemical studies on the cuticle and subcuticular structures in the strigeid trematode Cyathocotyle bushiensis. Proc. 1st Int. Congr. Parasitology, Rome 1964, Vol 1, pp. 493–494. Ed. Corradetti, A.. Pergamon Press: Tamburini Editore.CrossRefGoogle Scholar
Erasmus, D. A. (1967). The host-parasite interface of Cyathocotyle bushiensis Khan, 1962 (Trematoda: Strigeoidea). II. Electron microscope studies of the tegument. J. Parasit. 53, 703–14.Google Scholar
Erasmus, D. A. & Öhman, C. (1963). The structure and function of the adhesive organ in strigeid trematodes. Ann. N.Y. Acad. Sci. 113, 7–35.Google Scholar
Erasmus, D. A. & Öhman, C. (1965). Electron microscope studies of the gland cells and host-parasite interface of the adhesive organ of Cyathocotyle bushiensis Khan, 1962. J. Parasit. 51, 761–9.CrossRefGoogle ScholarPubMed
De Duve, C. & Wattiaux, R. (1966). Functions of lysosomes. A. Rev. Physiol. 28, 435–92.CrossRefGoogle ScholarPubMed
Holt, S. J. & Hicks, R. M. (1961). The localization of acid phosphatase in rat liver cells as revealed by combined cytochemical staining and electron microscopy. J. biophys. biochem. Cytol. 11, 47–66.Google Scholar
Lawn, A. M. (1960). The use of potassium permanganate as an electron dense stain for sections of tissue embedded in epoxy resin. J. biophys. biochem. Cytol. 7, 197–8.Google Scholar
Lee, D. L. (1966). The structure and composition of the helminth cuticle. In Adv. Parasitol. 4, 187–254. Ed. Dawes, B.. New York: Academic Press.Google Scholar
Luft, J. H. (1961). Improvements in epoxy resin embedding methods. J. biophys. biochem. Cytol. 9, 409–14.CrossRefGoogle ScholarPubMed
Molbert, E. R. G., Duspiva, F. & von Deimling, O. H. (1960). The demonstration of alkaline phosphatase in the electron microscope. J. biophys. biochem. Cytol. 7, 387–90.CrossRefGoogle Scholar
Normann, T. C. (1964). Staining thin sections with lead hydroxide without contamination by precipitated lead carbonate. Stain Technol. 39, 50–2.Google Scholar
Ogawa, K., Masutani, K. & Shinonaga, Y. (1962). Electron histochemical demonstration of acid phosphatase in the normal rat jejunum. J. Histochem. Cytochem. 10, 228–9.Google Scholar
Öhman, C. (1964). Comparative histological and histochemical studies of the Strigeida (Trematoda) with special reference to the structure and function of the adhesive organ. Ph.D. Thesis. University of Wales.Google Scholar
Read, C. P. (1966). Nutrition of intestinal helminths. In Biology of Parasites, pp. 101–26. Ed. Soulsby, E. J. L.. New York: Academic Press Inc.Google Scholar
Reynolds, E. S. (1963). The use of lead citrate at high pH as an electron opaque stain in electron microscopy. J. Cell Biol. 17, 208–12.Google Scholar