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The Hybrid II assay: a sensitive and specific real-time hybridization assay for the diagnosis of Theileria parva infection in Cape buffalo (Syncerus caffer) and cattle

Published online by Cambridge University Press:  09 September 2011

RONEL PIENAAR ,
FRED T. POTGIETER ,
ABDALLA A. LATIF ,
ORIEL M. M. THEKISOE  and
BEN J. MANS
RONEL PIENAAR
Affiliation:
Parasites, Vectors and Vector-Borne Diseases, Agricultural Research Council-Onderstepoort Veterinary Institute, Onderstepoort, 0110, South Africa Parasitology Research Program, Department of Zoology and Entomology, University of the Free State Qwaqwa Campus, Private Bag X13, Phuthaditjhaba, 9866, South Africa
FRED T. POTGIETER
Affiliation:
Parasites, Vectors and Vector-Borne Diseases, Agricultural Research Council-Onderstepoort Veterinary Institute, Onderstepoort, 0110, South Africa
ABDALLA A. LATIF
Affiliation:
Parasites, Vectors and Vector-Borne Diseases, Agricultural Research Council-Onderstepoort Veterinary Institute, Onderstepoort, 0110, South Africa The Department of Veterinary Tropical Diseases, University of Pretoria, Pretoria, South Africa
ORIEL M. M. THEKISOE
Affiliation:
Parasitology Research Program, Department of Zoology and Entomology, University of the Free State Qwaqwa Campus, Private Bag X13, Phuthaditjhaba, 9866, South Africa
BEN J. MANS*
Affiliation:
Parasites, Vectors and Vector-Borne Diseases, Agricultural Research Council-Onderstepoort Veterinary Institute, Onderstepoort, 0110, South Africa The Department of Veterinary Tropical Diseases, University of Pretoria, Pretoria, South Africa
*
*Corresponding author: Parasites, Vectors and Vector-Borne Diseases, Agricultural Research Council-Onderstepoort Veterinary Institute, Onderstepoort 0110, South Africa. Tel: +27 12 5299200. Fax: +27 12 5299434. E-mail: mansb@arc.agric.za
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Summary

Corridor disease is an acute, fatal disease of cattle caused by buffalo-adapted Theileria parva. This is a nationally controlled disease in South Africa and strict control measures apply for the movement of buffalo, which includes mandatory testing for the presence of T. parva and other controlled diseases. Accurate diagnosis of the T. parva carrier state in buffalo using the official real-time hybridization PCR assay (Sibeko et al.2008), has been shown to be affected by concurrent infection with T. sp. (buffalo)-like parasites. We describe the Hybrid II assay, a real-time hybridization PCR method, which compares well with the official hybridization assay in terms of specificity and sensitivity. It is, however, not influenced by mixed infections of T. sp. (buffalo)-like parasites and is as such a significant improvement on the current hybridization assay.

Keywords

Corridor diseasediagnostic assayreal-time hybridizationSyncerus cafferTheileria parva
Type
Research Article
Information
Parasitology , Volume 138 , Issue 14 , December 2011 , pp. 1935 - 1944
Copyright
Copyright © Cambridge University Press 2011

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