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Immunity to coccidiosis: effect of serum antibodies on cell invasion by sporozoites of Eimeria in vitro

Published online by Cambridge University Press:  06 April 2009

P. L. Long  and
M. Elaine Rose
P. L. Long
Affiliation:
Houghton Poultry Research Station, Houghton, Huntingdon
M. Elaine Rose
Affiliation:
Houghton Poultry Research Station, Houghton, Huntingdon
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Extract

A method for detecting antibody activity in vitro against the invasive stages of E. tenella and E. maxima is described. Treatment of cultured chick kidney monolayers with serum (or a globulin fraction thereof) from immunized birds caused a reduction in the numbers of intracellular stages 24 or 48 h after inoculation of sporozoites. This reduction was seen when serum was incorporated in the culture medium at the time of sporozoite inoculation and also, but to a lesser extent, when the monolayers had been pretreated with the antibody-containing medium which was washed off prior to infection. The results correlated reasonably well with those obtained when the sera were tested in embryo (E. tenella) or in vivo (E. maxima). Similar treatment of cultured CAM monolayers in which infection with E. tenella was well established (101 h), did not greatly affect the development of the parasite. This suggests that the anti-coccidial activity is either effective against the sporozoite stages only, or is unable to affect parasites within cells.

Type
Research Article
Information
Parasitology , Volume 65 , Issue 3 , December 1972 , pp. 437 - 445
Copyright
Copyright © Cambridge University Press 1972

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