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In vitro hatching of Hymenolepis diminuta eggs in Tenebrio molitor extracts and in defined enzyme preparations

Published online by Cambridge University Press:  06 April 2009

R. C. Lethbridge
R. C. Lethbridge
Affiliation:
Department of Zoology, Imperial College, London, S. W.7*
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Extract

The results of several experiments that included variation of the pH and concentration of the extracts and analysis of the role of enzymes separated from midgut fluid by electrophoresis indicated that hatching involved two or more proteolytic enzymes that digested the cytoplasmic layer and embryophore. Amylases from T. molitor and other sources were shown to play no part in hatching. A defined medium based on analyses of midgut fluid and containing trypsin, chymotrypsin and peptidases was shown to digest the embryophore and effect the release of hexacanths in a time comparable to that obtained with T. molitor extracts. The specificity of the enzymes attacking the cytoplasmic layer remains unknown.

Type
Research Article
Information
Parasitology , Volume 64 , Issue 3 , June 1972 , pp. 389 - 400
Copyright
Copyright © Cambridge University Press 1972

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References

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