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Isoelectric focusing of general protein and specific enzymes from pathotypes of Globodera rostochiensis and G. pallida

Published online by Cambridge University Press:  06 April 2009

P. C. Fox  and
H. J. Atkinson
P. C. Fox
Affiliation:
Department of Pure and Applied Zoology, The University of Leeds, Leeds LS2 9JT
H. J. Atkinson
Affiliation:
Department of Pure and Applied Zoology, The University of Leeds, Leeds LS2 9JT
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Summary

Variations in the general protein and 40 enzymes of the 8 European pathotypes of the potato cyst nematode Globodera rostochiensis and G. pallida have been examined by isoelectric focusing (IEF) and cluster analysis. Differences in the protein patterns were observed and the presence of a major band at pH 8·0 allowed the two species to be discriminated. Extra isozymed of peptidase (EC 3.4. 11.*) and esterase (EC 3. 1. 1. 1) were also found in G. rostochiensis. Intra-species variation was seen within G. rostochiensis for superoxide dismutase (EC 1. 15. 1. 1) and within G. pallida for phosphoglucomutase (EC 2. 7. 5. 1) and glucose phosphate isomerase (EC 5. 3. 1. 9). The results suggest that the two species are very similar and the prospect for recognizing their pathotypes by IEF is discussed.

Type
Research Article
Information
Parasitology , Volume 88 , Issue 1 , February 1984 , pp. 131 - 139
Copyright
Copyright © Cambridge University Press 1984

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References

REFERENCES

Berge, J.-B., Dalmasso, A., Person, F., Rivoal, R. & Thomas, D. (1981). Isoesterases chez le némattode Heterodera avenae. I. Polymorphisme chez différentes races franĉaises. Revue Nematologie 4, 99–105.Google Scholar
Bird, A. F. (1966). Esterases in the genus Meloidogyne. Nematologica 12, 359–61.Google Scholar
Burdett, P. E. & Whitehead, P. H. (1977). The separation of the phenotypes of phosphoglucomutase, erythrocyte acid phosphatase and some haemoglobin variants by isoelectric focusing. Analytical Biochemistry 77, 419–28.Google Scholar
Delincée, H. & Radola, B. J. (1978). Determination of isoelectric points in thin-layer isoelectric focusing: the importance of attaining the steady state and the role of CO2 interference. Analytical Biochemistry 90, 609–23.Google Scholar
Dickson, D. W., Huisingh, D. & Sasser, J. N. (1971). Dehydrogenases, acid and alkaline phosphatases and esterases for chemotaxonomy of selected Meloidogyne, Ditylenchus, and Aphelenchus spp. Journal of Nematology 3, 1–16.Google Scholar
Dunn, G. & Everitt, B. S. (1982). An Introduction to Mathematical Taxonomy. Cambridge University Press.Google Scholar
Ellenby, C. (1948). Resistance to the potato-root eelworm. Nature 162, 704.CrossRefGoogle Scholar
Ellenby, C. (1952). Resistance to the potato-root eelworm, Heterodera rostochiensis Wollenweber. Nature 170, 1016.Google Scholar
Evans, K. & Franco, J. (1977). Morphological variations in some populations of potato cyst-nematodes from Europe and South America. Nematologica 23, 417–30.Google Scholar
Ferguson, A. (1980). Biochemical Systematics and Evolution. Glasgow and London: Blackie.Google Scholar
Franco, J. (1978). Electrophoretic protein patterns of different populations of Globodera pallida and G. rostochiensis. Journal of Nematology 10, 287.Google Scholar
Franco, J. (1979). Disc-electrophoresis of female proteins of British and Peruvian potato cyst-nematode populations, Globodera spp. Nematologica 25, 32–5.Google Scholar
Giebel, J. (1974). Biochemical mechanisms of plant resistance to nematodes-a review. Journal of Nematology 6, 175–84.Google ScholarPubMed
Greet, D. N. & Firth, J. (1977). Influence of host plant on electrophoretic protein patterns of some round-cyst nematode females and use of larvae to obtain less ambiguous results. Nematologica 23, 411–15.Google Scholar
Harris, H. & Hopkinson, D. A. (1976). Handbook of Enzyme Electrophoresis in Human Genetics. Amsterdam: North-Holland.Google Scholar
Jones, F. G. W., Parrott, D. M. & Perry, J. N. (1981). The gene-for-gene relationship and its significance for potato cyst nematodes and their solanaceous hosts. In Plant Parasitic Nematodes, vol. 3 (ed. Zuckerman, B. M. and Rhode, R. A.), pp. 23–36. New York: Academic Press.Google Scholar
Keen, N. T. (1982). Specific recognition in gene-for-gene host-parasite systems. In Advances in Plant Pathology, vol. 1 (ed. Ingram, D. S. and Williams, P. H.), pp. 35–82. London: Academic Press.Google Scholar
Kort, J., Ross, H., Rumpenhorst, H. J. & Stone, A. R. (1977). An international scheme for identifying and classifying pathotypes of potato cyst-nematodes G. rostochiensis and G. pallida, Nematologica 23, 333–9.CrossRefGoogle Scholar
Leonard, K. J. & Czochor, R. J. (1980). Theory of genetic interactions among populations of plants and their pathogens. Annual Review of Phytopathology 18, 237–58.Google Scholar
Nummi, M., Niku-Paavola, M. L., Enari, T.-M., & Raunio, V. (1981). Isolation of cellulases by means of biospecific sorption on amorphous cellulose. Analytical Biochemistry 116, 137–41.CrossRefGoogle ScholarPubMed
Sarich, V. (1977). Rates, sample sizes, and the neutrality hypothesis for electrophoresis in evolutionary studies. Nature 265, 24–8.CrossRefGoogle ScholarPubMed
Shepherd, A. M. (1970). Extraction and estimation of Heterodera. In Laboratory Methods for Work with Plant and Soil Nematodes, MAFF Technical Bulletin, no. 2 (ed. Southey, J. F.), pp. 22–23. London: HMSO.Google Scholar
Smith, I. (1976). Chromatographic and Electrophoretic Techinques, vol. 2, Zone Electrophoresis. London: Heinemann.Google Scholar
Stone, A. R. & Williams, T. D. (1974). The morphology and soluble protein electrophoresis of Heterodera avenae pathotypes. Annals of Applied Biology 76, 231–6.Google Scholar
Toxopeus, H. J. & Huijsman, C. A. (1953). Breeding for resistance to potato root eelworm, Euphytica 2, 180–6.Google Scholar
Trudgill, D. L. & Parrott, D. M. (1972). Disc electrophoresis and larval dimensions of British, Dutch and other populations of H. rostochiensis as evidence of the existence of two species each with pathotypes. Nematologica 18, 141–8.Google Scholar
Wharton, R. J., Storey, R. M. J. & Fox, P. C. (1983). The potential of some immunochemical and biochemical approaches to the taxonomy of potato cyst-nematodes. In Concepts in Nematode Systematics (ed. Stone, A. R., Platt, H. M. and Khalil, L. K.). New York and London: Academic Press (in the Press).Google Scholar
Wishart, D. (1978). Clustan; User Manual. Edinburgh University: Program Library Unit.Google Scholar