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Oleic acid is indispensable for intraerythrocytic proliferation of Plasmodium falciparum

Published online by Cambridge University Press:  05 July 2007

F. MI-ICHI ,
S. KANO  and
T. MITAMURA
F. MI-ICHI
Affiliation:
Department of Molecular Protozoology, Research Institute for Microbial Diseases, Osaka University, 3-1 Yamadaoka, Suita, Osaka 565-0871, Japan
S. KANO
Affiliation:
Research Institute, International Medical Center of Japan, 1-21-1 Toyama, Shinjuku, Tokyo 162-8655, Japan
T. MITAMURA*
Affiliation:
Department of Molecular Protozoology, Research Institute for Microbial Diseases, Osaka University, 3-1 Yamadaoka, Suita, Osaka 565-0871, Japan
*
*Corresponding author: Research Institute, International Medical Center of Japan, 1-21-1 Toyama, Shinjuku, Tokyo 162-8655, Japan. Tel: +81-3-3202-7181 ext. 2871. Fax: +81-3-3202-7364. E-mail: tmitamura@ri.imcj.go.jp
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Summary

Serum-derived fatty acids are essential for the intraerythrocytic proliferation of Plasmodium falciparum in humans. We previously reported that only limited combinations of fatty acids can support long-term parasite culture, and palmitic acid (C16:0)/oleic acid (C18:1, n-9), palmitic acid (C16:0)/vaccenic acid (C18:1, n-7), or stearic acid (C18:0) are required in these combinations, implying that these fatty acids are key molecules for intraerythrocytic parasite growth (Mi-Ichi et al.2006). Here, we analysed profiles of parasitaemia changes as well as morphologies during the erythrocytic cycle and confirmed the importance of C16:0 and C18:1, n-9. We also provide evidence that C18:1, n-9 but not other C18 monoenoic or dienoic acids maintain the synchronicity of parasite development in serum-free medium when paired with C16:0, resulting in maintained exponential growth. Thus, C18:1, n-9 is indispensable for the intraerythrocytic proliferation of P. falciparum.

Keywords

malarialipid metabolismfatty acidcell cycleapicomplexa
Type
Research Article
Information
Parasitology , Volume 134 , Issue 12 , November 2007 , pp. 1671 - 1677
Copyright
Copyright © Cambridge University Press 2007

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