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Origin, kinetics of circulation and fate in vivo of the major excretory–secretory product of Acanthocheilonema viteae

Published online by Cambridge University Press:  06 April 2009

W. Harnett ,
M. J. Worms ,
A. Kapil ,
M. Grainger  and
R. M. E. Parkhouse
W. Harnett
Affiliation:
National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA
M. J. Worms
Affiliation:
National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA
A. Kapil
Affiliation:
National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA
M. Grainger
Affiliation:
National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA
R. M. E. Parkhouse
Affiliation:
Divisions of Parasitology and Immunology, The Ridgeway, Mill Hill, London NW7 1AA
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Summary

The excretions-secretions (E-S) of Acanthocheilonema viteae consist mainly of one product, molecular weight 62 kDa. This molecule is synthesized during the vertebrate phase of the parasite life-cycle and is first detectable in the E-S of L4 parasites. It is cross-reactive with E-S of human filarial parasites as a consequence of possessing a phosphorylcholine (PC) moiety. The 62 kDa molecule has been employed as a model for the study of the origin and fate of filarial E-S. Immunohistological analysis has shown the molecule to be located predominantly in the parasite gut. Transplantation of adult female [S] methionine pulsed worms into uninfected jirds resulted in the radio-labelled secreted 62 kDa antigen being detected in the bloodstream within 4 h by SDS–PAGE/immunoprecipitation analysis. The systemic half-life of the molecule as estimated by clearance of injected, purified I-labelled material was measured in naive and infected jird hosts.

Keywords

Acanthocheilonema viteaeexcretory–secretory productkinetics of circulation
Type
Research Article
Information
Parasitology , Volume 99 , Issue 2 , October 1989 , pp. 229 - 239
Copyright
Copyright © Cambridge University Press 1989

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