Hostname: page-component-7479d7b7d-q6k6v Total loading time: 0 Render date: 2024-07-13T15:43:19.708Z Has data issue: false hasContentIssue false
  • English
  • Français

Randomly amplified polymorphic DNA analysis of Trypanosoma rangeli and allied species from human, monkeys and other sylvatic mammals of the Brazilian Amazon disclosed a new group and a species-specific marker

Published online by Cambridge University Press:  03 March 2004

F. MAIA DA SILVA ,
A. C. RODRIGUES ,
M. CAMPANER ,
C. S. A. TAKATA ,
M. C. BRIGIDO ,
A. C. V. JUNQUEIRA ,
J. R. COURA ,
G. F. TAKEDA ,
J. J. SHAW  and
M. M. G. TEIXEIRA
F. MAIA DA SILVA
Affiliation:
Department of Parasitology, Institute of Biomedical Science, University of São Paulo, São Paulo, 05508-900, Brazil
A. C. RODRIGUES
Affiliation:
Department of Parasitology, Institute of Biomedical Science, University of São Paulo, São Paulo, 05508-900, Brazil
M. CAMPANER
Affiliation:
Department of Parasitology, Institute of Biomedical Science, University of São Paulo, São Paulo, 05508-900, Brazil
C. S. A. TAKATA
Affiliation:
Department of Parasitology, Institute of Biomedical Science, University of São Paulo, São Paulo, 05508-900, Brazil
M. C. BRIGIDO
Affiliation:
Department of Parasitology, Institute of Biomedical Science, University of São Paulo, São Paulo, 05508-900, Brazil
A. C. V. JUNQUEIRA
Affiliation:
Department of Tropical Medicine, Instituto Oswaldo Cruz, FIOCRUZ, Rio de Janeiro, 21045-900, Brazil
J. R. COURA
Affiliation:
Department of Tropical Medicine, Instituto Oswaldo Cruz, FIOCRUZ, Rio de Janeiro, 21045-900, Brazil
G. F. TAKEDA
Affiliation:
Department of Parasitology, Institute of Biomedical Science, University of São Paulo, São Paulo, 05508-900, Brazil
J. J. SHAW
Affiliation:
Department of Parasitology, Institute of Biomedical Science, University of São Paulo, São Paulo, 05508-900, Brazil
M. M. G. TEIXEIRA
Affiliation:
Department of Parasitology, Institute of Biomedical Science, University of São Paulo, São Paulo, 05508-900, Brazil
Get access Rights & Permissions [Opens in a new window]

Abstract

We characterized 14 trypanosome isolates from sylvatic mammals (9 from primates, 1 from sloth, 2 from anteaters and 2 from opossum) plus 2 human isolates of Brazilian Amazon. These isolates were proven to be Trypanosoma rangeli by detection of metacyclic trypomastigotes in the salivary glands of triatomines and by a specific PCR assay. Polymorphism determined by randomly amplified polymorphic DNA (RAPD) revealed that most (12) of the Brazilian T. rangeli isolates from the Amazon differed from those of other geographical regions, thus constituting a new group of T. rangeli. Four Brazilian isolates clustered together with a previously described group (A) that was described as being composed of being isolates from Colombia and Venezuela. Isolates from Panama and El Salvador form another group. The isolate from Southern Brazil did not cluster to any of the above-mentioned groups. This is the first study that assesses the genetic relationship of a large number of isolates from wild mammals, especially from non-human primates. A randomly-amplified DNA fragment (Tra625) exclusive to T. rangeli was used to develop a PCR assay able to detect all T. rangeli groups.

Keywords

Trypanosoma rangelimonkeySouth American trypanosomesBrazilian AmazonHerpetosomaRAPDgenetic relatednesswild mammalsPCRdiagnosis
Type
Research Article
Information
Parasitology , Volume 128 , Issue 3 , March 2004 , pp. 283 - 294
Copyright
2004 Cambridge University Press

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

AÑEZ, N. (1982). Studies on Trypanosoma rangeli Tejera, 1920. IV – A reconsideration of its systematic position. Memórias do Instituto Oswaldo Cruz 77, 405–415.CrossRefGoogle Scholar
COURA, J. R., FERNANDES, O., ABOLEDA, M., BARETT, T. V., CARRARA, N., DEGRAVE, W. & CAMPBELL, D. A. (1996). Human infection by Trypanosoma rangeli in the Brazilian Amazon. Transactions of the Royal Society of Tropical Medicine and Hygiene 90, 278–279.CrossRefGoogle Scholar
D'ALESSANDRO, A. (1976). Biology of Trypanosoma (Herpetosoma) rangeli Tejera, 1920. In Biology of the Kinetoplastida (ed. Lumsden, W. H. R. & Evans, D. A.), pp. 327–403. Academic Press, London.
D'ALESSANDRO, A. & SARAIVA, N. G. (1992). Trypanosoma rangeli. In Parasitic Protozoa (ed. Kreier, J. & Baker, J. R.), pp. 1–54. Academic Press, New York.
D'ALESSANDRO, A. & SARAIVA, N. G. (1999). Trypanosoma rangeli. In Protozoal Diseases (ed. Gilles, H. M.), pp. 398–412. Edward Arnold, London.
DEANE, L. M., ALMEIDA, F. B., NETO, J. A. F. & DA SILVA, J. E. (1972). Trypanosoma cruzi e outros tripanossomas em primatas brasileiros. Revista da Sociedade Brasileira de Medicina Tropical 6, 361.Google Scholar
DEANE, L. M. & DAMASCENO, R. R. (1961). Tripanosomatídeos de mamíferos da região amazônica. II. Tripanosomas de macacos da zona de salgado, estado do Pará. Revista do Instituto de Medicina Tropical de São Paulo 3, 61–70.Google Scholar
DEREURE, J., BARNABE, C., VIE, J. C., MADELENAT, F. & RACCURT, C. (2001). Trypanosomatidae from wild mammals in the neotropical rainforest of French Guiana. Annals of Tropical Medicine and Parasitology 95, 157–166.CrossRefGoogle Scholar
DE THOISY, B., VOGEL, I., REYNES, J. M., POULIQUEN, J. F., CARME, B., KAZANJI, M. & VIE, J. C. (2001). Health evaluation of translocated free-ranging primates in French Guiana. American Journal of Primatology 54, 1–16.CrossRefGoogle Scholar
GRISARD, E. C., CAMPBELL, D. A. & ROMANHA, A. J. (1999). Mini-exon gene sequence polymorphism among Trypanosoma rangeli isolated from distinct geographical regions. Parasitology 118, 375–382.CrossRefGoogle Scholar
GUHL, F. & VALLEJO, G. A. (2003). Trypanosoma (Herpetosoma) rangeli Tejera, 1920 – An updated review. Memórias do Instituto Oswaldo Cruz 98, 435–442.CrossRefGoogle Scholar
HECKER, H., SCHWARZENBACH, M. & RUDIN, W. (1990). Development and interactions of Trypanosoma rangeli in and with the reduviid bug Rhodnius prolixus. Parasitology Research 76, 311–318.CrossRefGoogle Scholar
HOARE, C. A. (1972). The Trypanosomes of Mammals. Blackwell, Oxford.
MACHADO, P. E., EGER-MANGRICH, I., ROSA, G., KOERICH, L. B., GRISARD, E. C. & STEINDEL, M. (2001). Differential susceptibility of triatomines of the genus Rhodnius to Trypanosoma rangeli strains from different geographical origins. International Journal for Parasitology 31, 632–634.CrossRefGoogle Scholar
MARINKELLE, C. J. (1976). The biology of the trypanosomes of non-human primates. In Biology of the Kinetoplastida (ed. Lumsden, W. H. R. & Evans, D. A.), pp. 218–248. Academic Press, London.
MILES, M. A., ARIAS, J. R., VALENTE, S. A. S., NAIFF, R. D., DE SOUZA, A. A., POVOA, M. M., LIMA, J. A. N. & CEDILLOS, R. A. (1983). Vertebrate hosts and vectors of Trypanosoma rangeli in the Amazon basin of Brasil. American Journal of Tropical Medicine and Hygiene 32, 1251–1259.CrossRefGoogle Scholar
MORALES, L., ROMERO, I., DIEZ, H., DEL PORTILLO, P., MONTILLA, M., NICHOLLS, S. & PUERTA, C. (2002). Characterization of a candidate Trypanosoma rangeli small nucleolar RNA gene and its application in a PCR-based parasite detection. Experimental Parasitology 102, 72–80.CrossRefGoogle Scholar
RAMIREZ, L. E., LAGES-SILVA, E., ALVARENGA-FRANCO, F., MATOS, A., VARGAS, N., FERNANDES, O. & ZINGALES, B. (2002). High prevalence of Trypanosoma rangeli and Trypanosoma cruzi in opossums and triatomids in a formerly-endemic area of Chagas disease in Southeast Brazil. Acta Tropica 84, 189–198.CrossRefGoogle Scholar
RAMIREZ, L. E., MACHADO, M. I., MAYWALD, P. G., MATOS, A ., CHIARI, E. & SILVA, E. L. (1998). Primeira evidência de Trypanosoma rangeli no sudeste do Brasil, região endêmica para doença de Chagas. Revista da Sociedade Brasileira de Medicina Tropical 31, 99–102.CrossRefGoogle Scholar
SERRANO, M. G., CAMARGO, E. P. & TEIXEIRA, M. M. G. (1999). Phytomonas: analysis of polymorphism and genetic relatedness between isolates from plants and phytophagous insects from different geographic regions by RAPD fingerprints and synapomorphic markers. Journal of Eukaryotic Microbiology 46, 618–625.CrossRefGoogle Scholar
SERRANO, M. G., CAMPANER, M., BUCK, G. A., TEIXEIRA, M. M. G. & CAMARGO, E. P. (1999). PCR amplification of the spliced leader gene for the diagnosis of trypanosomatid parasites of plants and insects in methanol-fixed smears. FEMS Microbiology Letters 176, 241–246.CrossRefGoogle Scholar
SHAW, J. J. (1985). The hemoflagellates of sloths, vermilinguas (anteaters), and armadillos. In The Evolution and Ecology of Armadillos, Sloths and Vermilingues (ed. Montegomery, G. G.), pp. 279–292. Smithsonian Institute Press, Washington DC.
SILVA, F. M., RODRIGUES, A. C., CAMPANER, M., FERREIRA, R. C., TAKEDA, G. F. & TEIXEIRA, M. M. G. (1999). Trypanosoma (Herpetosoma) spp.: genetic diversity and taxonomic position defined by ribosomal, spliced leader and RAPD markers. Memorías do Instituto Oswaldo Cruz 94 (Suppl. II), 158.Google Scholar
SOUSA, O. E. & DAWSON, G. A. (1976). Trypanosome infections in the marmoset (Saguinus geoffroyi) from the Panama Canal Zone. American Journal of Tropical Medicine and Hygiene 25, 407–409.CrossRefGoogle Scholar
SOUSA, O. E., ROSSAN, R. N. & BAERG, D. C. (1974). The prevalence of trypanosomes and microfilariae in Panamanian monkeys. American Journal of Tropical Medicine and Hygiene 23, 862–869.CrossRefGoogle Scholar
SOUTO, R. P., VARGAS, N. & ZINGALES, B. (1999). Trypanosoma rangeli: discrimination from Trypanosoma cruzi based on a variable domain from the large subunit ribosomal RNA gene. Experimental Parasitology 91, 306–314.CrossRefGoogle Scholar
STEINDEL, M., CARVALHO PINTO, J. C., TOMA, H. K., MANGIA, R. H. R., RIBEIRO-RODRIGUES, R. & ROMANHA, A. J. (1991). Trypanosoma rangeli (Tejera, 1920) isolated from sylvatic rodent (Echimys dasytrix) in Santa Catarina island, Santa Catarina state: first report of this trypanosome in Southern Brazil. Memórias do Instituto Oswaldo Cruz 86, 73–79.CrossRefGoogle Scholar
STEINDEL, M., DIAS NETO, E., PINTO, C. J., GRISARD, E. C., MENEZES, C. L., MURTA, S. M., SIMPSON, A. J. & ROMANHA, A. J. (1994). Randomly amplified polymorphic DNA (RAPD) and isoenzyme analysis of Trypanosoma rangeli strains. Journal of Eukaryotic Microbiology 41, 261–267.CrossRefGoogle Scholar
STEVENS, J. R., NOYES, H. A., SCHOFIELD, C. J. & GIBSON, W. (2001). The molecular evolution of Trypanosomatidae. Advances in Parasitology 48, 1–56.CrossRefGoogle Scholar
STEVENS, J. R., TEIXEIRA, M. M. G., BINGLE, L. E. & GIBSON, W. C. (1999). The taxonomic position and evolutionary relationships of Trypanosoma rangeli. International Journal for Parasitology 29, 749–757.CrossRefGoogle Scholar
VALLEJO, G. A., GUHL, F., CARRANZA, J. C., LOZANO, L. E., SÁNCHEZ, J. L., JARAMILLO, J. C., GUALTERO, D., CASTAÑEDA, N., SILVA, J. C. & STEINDEL, M. (2002). KDNA markers define two major Trypanosoma rangeli lineages in Latin-America. Acta Tropica 81, 77–82.CrossRefGoogle Scholar
VALLEJO, G. A., GUHL, F., CARRANZA, J. C., MORENO, J., TRIANA, O. & GRISARD, E. C. (2003). Parity between kinetoplast DNA and mini-exon gene sequences supports either clonal evolution or speciation in Trypanosoma rangeli strains isolated from Rhodnius colombiensis, R. pallescens and R. prolixus in Colombia. Infection, Genetics and Evolution 3, 39–45.CrossRefGoogle Scholar
VALLEJO, G. A., GUHL, F., CHIARI, E. & MACEDO, A. M. (1999). Species specific detection of Trypanosoma cruzi and Trypanosoma rangeli in vector and mammalian hosts by polymerase chain reaction amplification of kinetoplast minicircle DNA. Acta Tropica 72, 203–212.CrossRefGoogle Scholar
VARGAS, N., SOUTO, R. P., CARRANZA, J. C., VALLEJO, G. A. & ZINGALES, B. (2000). Amplification of a specific repetitive DNA sequence for Trypanosoma rangeli identification and its potential application in epidemiological investigations. Experimental Parasitology 96, 147–159.CrossRefGoogle Scholar
VENTURA, R. M., TAKATA, C. S. A., SILVA, R. A. M. S., NUNES, V. L., TAKEDA, G. F. & TEIXEIRA, M. M. G. (2000). Molecular and morphological studies of Brazilian Trypanosoma evansi stocks: the total absence of kDNA in trypanosomes from both laboratory stocks and naturally infected domestic and wild mammals Journal of Parasitology 86, 1289–1298.Google Scholar
ZICCARDI, M. & OLIVEIRA, R. L. (1998). Morphological features of trypanosomes in squirrel monkeys at two sites in Brazilian Amazon. Memórias do Instituto Oswaldo Cruz 92, 465–470.Google Scholar
ZICCARDI, M., OLIVEIRA, R. L., LAINSON, R., BRÍGIDO, M. C. O. & MUNIZ, J. A. P. C. (2000). Trypanosomes of non-human primates from the national center of primates, Ananindeua, State of Pará, Brazil. Memórias do Instituto Oswaldo Cruz 95, 157–159.CrossRefGoogle Scholar