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Separation of erythrocytes infected with murine malaria parasites in metrizamide gradients

Published online by Cambridge University Press:  06 April 2009

Elsie M. Eugui  and
A. C. Allison
Elsie M. Eugui
Affiliation:
Division of Cell Pathology, Clinical Research Centre, Watford Road, Harrow, Middlesex HA1 3UJ
A. C. Allison
Affiliation:
Division of Cell Pathology, Clinical Research Centre, Watford Road, Harrow, Middlesex HA1 3UJ
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Summary

Density gradients with metrizamide, a tri-iodinated benzamido derivative of glucose, have been used to separate erythrocytes infected with 3 species of murine plasmodia. Good separations were obtained of uninfected erythrocytes from erythrocytes containing parasites in different developmental stages. With metrizamide solutions, the densities required for isopycnic separation can be obtained without hypertonicity or high viscosity, and the viability and those aspects of the metabolism of parasites and erythrocytes that we measured are not detectably modified by exposure to these solutions. This type of separation has many possible applications to biochemical and immunological investigations.

Type
Research Article
Information
Parasitology , Volume 79 , Issue 2 , October 1979 , pp. 267 - 275
Copyright
Copyright © Cambridge University Press 1979

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References

REFERENCES

Allison, A. C., Christensen, J., Clark, I. A., Elford, B. C. & Eugui, E. M. (1979). The role of the spleen in protection against murine Babesia infections. In The Spleen and its Role in Parasitic Infections (ed. Torrigiani, G.). Basel: Karger.Google Scholar
Carter, R. & Diggs, C. L. (1977). Plasmodia of rodents. In Parasitic Protozoa, vol. 3 (ed. Kreier, J. P.), pp. 359–466. New York and London: Academic Press.Google Scholar
Eling, W. (1977). Ficoll fractionation for the separation of parasitized erythrocytes from malaria infected blood. Bulletin of the World Health Organization 55, 105–14.Google ScholarPubMed
Kreier, J. P. (1977). The isolation and fractionation of malaria infected cells. Bulletin of the World Health Organization 55, 317–31.Google ScholarPubMed
Lunde, M. M. & Powers, K. S. (1976). The preparation of malaria haemagglutination antigen. Annals of Tropical Medicine and Parasitology 70, 283–91.CrossRefGoogle ScholarPubMed
McAllister, R. O. & Gordon, D. M. (1976). Schizont-infected cell enrichment in rodent malaria. Journal of Parasitology 62, 664–9.CrossRefGoogle Scholar
Miller, L. H. & Chien, S. (1971). Density distribution of red cells infected by Plasmodium knowlesi and Plasmodium coatneyi. Experimental Parasitology 29, 451–6.CrossRefGoogle ScholarPubMed
Mitchell, G. H., Butcher, G. A. & Cohen, S. (1973). Isolation of blood-stage merozoites from Plasmodium malaria. International Journal for Parasitology 3, 443–5.CrossRefGoogle Scholar
Munthe-Kaas, A. C. & Seglen, P. O. (1974). The use of metrizamide as a gradient medium for isopycnic separation of rat liver cells. Febs Letters 43, 252–6.CrossRefGoogle ScholarPubMed
Neame, K. D. & Homewood, C. A. (1975). Alterations in the permeability of mouse erythrocytes infected with the malaria parasite, Plasmodium berghei. International Journal for Parasitology 5, 537–40.CrossRefGoogle ScholarPubMed
Pasvol, G., Wilson, R. J. M., Smalley, M. E. & Brown, J. (1978). Separation of viable schizont-infected red cells of Plasmodium falciparum from human blood. Annals of Tropical Medicine and Parasitology 72, 87–8.CrossRefGoogle ScholarPubMed
Rickwoon, D. & Birnie, G. D. (1975). Metrizamide, a new density-gradient medium. Febs Letters 50, 102–10.CrossRefGoogle Scholar
Rowley, P. T., Siddiqui, W. A. & Geiman, Q. M. (1967). Separation of malarial parasites according to age by density gradient centrifugation. Journal of Laboratory and Clinical Medicine 70, 933–7.Google ScholarPubMed
Trager, W. & Jensen, J. B. (1977). Cultivation of erythrocytic stages. Bulletin of the world Health Organization 55, 363–5.Google ScholarPubMed
Williamson, J. & Cover, B. (1966). Separation of blood-cell-free trypanosomes and malaria parasites on a sucrose gradient. Transactions of the Royal Society of Tropical Medicine and Hygiene 60, 425–7.CrossRefGoogle Scholar