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Superoxide dismutase from Ascaris suum

Published online by Cambridge University Press:  06 April 2009

M. Sanchez-Moreno ,
M. Monteoliva ,
A. Fatou  and
M. A. García-Ruiz
M. Sanchez-Moreno
Affiliation:
Instituto ‘López-Neyra’ de Parasitología, C.S.I.C. Sección de Bioquímica, c/ Ventanilla 11, Granada-18002, Spain
M. Monteoliva
Affiliation:
Instituto ‘López-Neyra’ de Parasitología, C.S.I.C. Sección de Bioquímica, c/ Ventanilla 11, Granada-18002, Spain
A. Fatou
Affiliation:
Instituto ‘López-Neyra’ de Parasitología, C.S.I.C. Sección de Bioquímica, c/ Ventanilla 11, Granada-18002, Spain
M. A. García-Ruiz
Affiliation:
Instituto ‘López-Neyra’ de Parasitología, C.S.I.C. Sección de Bioquímica, c/ Ventanilla 11, Granada-18002, Spain
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Summary

Three superoxide dismutases (SOD) (EC 1.15.1.1) were detected in homogenates of Ascaris suum. Each of the three forms of SOD was purified by a sequence of multiple differential centrifugations, ammonium sulphate precipitation, ion-exchange chromatography and G-75 Sephadex column chromatography. The three forms of SOD were present in different cellular locations; one in the cytoplasmic fraction, sensitive to cyanide and hydrogen peroxide, and two in the mitochondrial fraction, one of which was cyanide sensitive. The SOD forms presented clear differences in their electrophoretic patterns. The sexual organs of females showed the highest SOD activities of all the tissues examined. The finding of such high levels of superoxide dismutase in A. suum reflects the importance of this enzyme in the metabolism of this helminth parasite.

Type
Research Article
Information
Parasitology , Volume 97 , Issue 2 , October 1988 , pp. 345 - 353
Copyright
Copyright © Cambridge University Press 1988

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