Hostname: page-component-7bb8b95d7b-2h6rp Total loading time: 0 Render date: 2024-10-04T06:23:25.050Z Has data issue: false hasContentIssue false
  • English
  • Français

The synthesis of a variant-specific antigen by Trypanosoma brucei in vitro

Published online by Cambridge University Press:  06 April 2009

D. W. Taylor  and
G. A. M. Cross
D. W. Taylor
Affiliation:
Medical Research Council Biochemical Parasitology Unit, Molteno Institute, Dowing Street, Cambridge CB2 3EE
G. A. M. Cross
Affiliation:
Medical Research Council Biochemical Parasitology Unit, Molteno Institute, Dowing Street, Cambridge CB2 3EE
Get access Rights & Permissions [Opens in a new window]

Extract

A variant-specific surface antigen from a cloned population of Trypanosoma brucei S 42 has been isolated and partically characterized. [35S]L-methionine was found to be incorporated into this material by cells incubated in vitro in a chemically defined medium. Incorporation of [35S]L-methionine was inhibited by cycloheximide and puromycin at concentrations which are known to specifically inhibit protein synthesis in other systems. The rate of synthesis of the variant-specific antigen in vitro has been estimated to be about 8% of the rate in vivo. Newly synthesized [35S]L-methionine-labelled variant-specific antigen was incorporated into the surface coat.

Type
Research Article
Information
Parasitology , Volume 74 , Issue 1 , February 1977 , pp. 47 - 60
Copyright
Copyright © Cambridge University Press 1977

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Bretscher, M. S. (1971). Human erythrocyte membrances: specific labelling of surface proteins. Journal of Molecular Biology 58, 775–81.CrossRefGoogle Scholar
Cross, G. A. M. (1975). Identification, purification and properties of clone-specific glycoprotein antigens constituting the surface coat of Trypanosoma brucei. Parasitology 71, 393–417.CrossRefGoogle ScholarPubMed
Cross, G. A. M. & Johnson, J. G. (1976). Structure and organisation of the varient-specific surface antigens of Trypanosoma brucei. 2nd International Symposium on the Biochemistry of Parasites and Host–Parasite Relationship (ed. Bossche, Van der), Amsterdam: Elsevier/North Holland.Google Scholar
Cross, G. A. M., Klein, R. A. & Linstead, D. J. (1975). Utilization of amino acids by Trypanosoma brucei in culture: L-threonine as a precursor for acetate. Parasitology 71, 311–26.CrossRefGoogle ScholarPubMed
Cross, G. A. M. & Manning, J. C. (1973). Cultivation of Trypanosoma brucei sspp. in semidefined and defined media. Parasitology 67, 315–31.CrossRefGoogle ScholarPubMed
Desowitz, R. S. (1970). African Tryanosomes. In Immunity to Parasitic Animals, Vol. 2 (ed. Jackson, G. J., Herman, R., Singer, I.), pp. 551–96. New York: Appleton-Century. Crofts.Google Scholar
Dwyer, D. M. (1976). Immunologic and fine structure evidence of avidly bound host serum proteins in the surface coat of a bloodstream trypanosome. Proceedings of the National Academy of Science, USA 73, 1222–6.CrossRefGoogle ScholarPubMed
Eveleigh, J. W. & Winter, G. D. (1970). Amino acid composition determination. In Protein Sequence Determination (Needleman, S. B.), pp. 91–123. London: Clapman and Hall.CrossRefGoogle Scholar
Ketteridge, D. S. (1970). The presence of host serum components on the surface of rodent adapted Trypanosoma vivax. Journal of Protozoology 17 (suppl.), 24.Google Scholar
Ketteridge, D. S. (1971). Studies on rodent adapted Trypanosoma vivax. Ph.D. thesis, University of Glasgow.Google Scholar
Lanham, S. M. (1968). Separation of trypanosoma from blood of infected rats and mice by anion exchangers. Nature, London 218, 1273–4.CrossRefGoogle ScholarPubMed
Lanham, S. M. & Godfrey, D. G. (1970). Isolation of salivarian trypanosoma from man and other animals using DEAE-cellulose. Experimental Parasitology 28, 521–34.CrossRefGoogle ScholarPubMed
Opperdoes, F. R., Borst, P. & Fonck, K. (1976). The potential use of inhibitors of glycerol-3-phosphate oxidase for chemotherapy of African Trypanosomiasis, FEBS Letters 62, 169–72.CrossRefGoogle ScholarPubMed
Ouchterlony, O. & Nilsson, L. A. (1973). Immunodiffusion and immunoelectrophoresis. In Handbook of Experimental Immunology, 2nd ed. (ed. Weir, D. M.), pp. 19.1–19.39. Oxford: Blackwell Scientific Publications.Google Scholar
Raadt, P. D. E (1974). Immunity and antigenic variation: clinical observations suggestive of immune phenomena in African trypanosomasis. In Trypanosomasis and Leishmaniasis with Special Reference to Changas' Disease. Ciba Fdn Symp. N.S. pp. 199–224. Amsterdam: Associated Scientific Publishers.CrossRefGoogle Scholar
Seed, J. R. (1974). Antigens and antigenic variability of the African trypanosomes. Journal of Protozoology 21, 639–46.CrossRefGoogle ScholarPubMed
Vickerman, K. (1969). On the surface structure and flagellar adhesion in trypanosomes. Journal of Cell Science 5, 163–93.CrossRefGoogle Scholar
Vickerman, K. (1974 a). The ultrastructure of pathogenic flagellates. In Trypanosomiasis and Leishmaniasis with special reference to Chagas' disease. Ciba Fdn Symp. 20 N.S., pp. 171–98. Amsterdam: Associated Scientific Publishers.CrossRefGoogle Scholar
Vickerman, K. (1969 b). Atigenic variation in African trypanosomes. In Parasites on the Immunised Host: Mechanisms of Survival. Ciba Fdn Symp. N.S. 25, pp. 53–80. Amsterdam: Associated Scientific Publishers.Google Scholar
Weber, K. & Osborn, M. (1969). The reliability of molecular weight determination by dodecyl sulphate-polyacrylamide gel electrophoresis. Journal of Biological Chemistry 244, 2206–12.CrossRefGoogle Scholar