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Tissue fluid in rabbits infected with Trypanosoma (Trypanozoon) brucei

Published online by Cambridge University Press:  06 April 2009

L. G. Goodwin  and
M. W. Guy
L. G. Goodwin
Affiliation:
Nuffield Institute of Comparative Medicine, The Zoological Society of London, Regent's Park, London NW1 4RY
M. W. Guy
Affiliation:
Nuffield Institute of Comparative Medicine, The Zoological Society of London, Regent's Park, London NW1 4RY
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Extract

Perforated plastic cylinders (hair curlers) were implanted subcutaneously in rabbits for the collection of tissue fluid. Using this technique, a study was made of the chemical constituents of tissue fluid and serum in rabbits infected with Trypanosoma (Trypanozoon) brucei, as compared with uninfected, control animals. Changes were caused by the infection in the concentrations of proteins, electrolytes, urea, creatinine, cholesterol and pyruvate, and in the activities of the enzymes aspartate transaminase, creatine phosphokinase and alkaline phosphatase. Antitrypanosomal antibody, detectable by the indirect fluorescent antibody technique, was shown to penetrate the tissue fluid and to reach a titre of about one-fifth of that in the serum. Renal failure, probably resulting from allergic reactions, was shown to be a contributory cause of death in rabbits infected with T. (T.) brucei.

Type
Research Article
Information
Parasitology , Volume 66 , Issue 3 , June 1973 , pp. 499 - 513
Copyright
Copyright © Cambridge University Press 1973

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