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Tracing the genus Sphaerospora: rediscovery, redescription and phylogeny of the Sphaerospora ranae (Morelle, 1929) n. comb. (Myxosporea, Sphaerosporidae), with emendation of the genus Sphaerospora

Published online by Cambridge University Press:  26 July 2007

M. JIRKŮ ,
I. FIALA  and
D. MODRÝ
M. JIRKŮ*
Affiliation:
Institute of Parasitology, Biology Centre, AS CR, Branišovská 31, 370 05 České Budějovice, Czech Republic Department of Parasitology, University of Veterinary and Pharmaceutical Sciences, Palackého 1-3, 612 42 Brno, Czech Republic
I. FIALA
Affiliation:
Institute of Parasitology, Biology Centre, AS CR, Branišovská 31, 370 05 České Budějovice, Czech Republic Faculty of Biological Sciences, University of South Bohemia, Branišovská 31, 370 05 České Budějovice, Czech Republic
D. MODRÝ
Affiliation:
Institute of Parasitology, Biology Centre, AS CR, Branišovská 31, 370 05 České Budějovice, Czech Republic Department of Parasitology, University of Veterinary and Pharmaceutical Sciences, Palackého 1-3, 612 42 Brno, Czech Republic
*
*Corresponding author: Institute of Parasitology, Biology Centre, AS CR, Branišovská 31, 370 05 České Budějovice, Czech Republic. Tel: +420 38 7775474. Fax: +420 38 5310388. E-mail: miloslav.jirku@seznam.cz
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Summary

Using a combination of morphological, life-history and molecular data, we redescribe Sphaerospora ranae (Morelle, 1929) n. comb. (previously Leptotheca ranae) and emend its taxonomic status. Renal infection was recorded in 2 spp. of frogs (out of 5 amphibian spp. examined), Rana dalmatina (proposed type host) and Rana temporaria, suggesting restricted host specifity of S. ranae. We provide a description of sporogonic stages of S. ranae for the first time and suggest possible modes of its developmental cycle. Phylogenetic analysis inferred from the small-subunit ribosomal DNA revealed a close relationship of S. ranae with piscine Sphaerospora elegans (type species of the genus) and Sphaerospora truttae, forming together with distantly related Leptotheca fugu a ‘Sphaerosporid clade’, the basal branch to all myxosporean species. The close relationship of the 3 Sphaerospora spp. is further supported by the presence of 2 areas with extensive nucleotide insertions in the V4 region of the SSU rDNA (absent in L. fugu), morphology and life-history features. We conclude, that the spore morphology of Sphaerospora s.l., is very simple and probably represents a ‘primitive’, basal morphotype retained in most myxosporean lineages. Based on presented data, we propose emendation of the genus Sphaerospora using morpological, life-history and molecular features.

Keywords

MyxosporeaSphaerospora ranae n. comb.LeptothecaAmphibiaRanaredescriptionphylogenySSU rDNAgenus emendationmolecular characteristic
Type
Research Article
Information
Parasitology , Volume 134 , Issue 12 , November 2007 , pp. 1727 - 1739
Copyright
Copyright © Cambridge University Press 2007

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