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The ultrastructure of the adult stage of Trichostrongylus colubriformis and Haemonchus placei

Published online by Cambridge University Press:  06 April 2009

Kenneth Smith  and
Eric Harness
Kenneth Smith
Affiliation:
Agricultural Research Council, Institute for Research on Animal Diseases, Compton, Newbury, Berks.
Eric Harness
Affiliation:
Agricultural Research Council, Institute for Research on Animal Diseases, Compton, Newbury, Berks.
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Extract

The ultrastructure of the adult stage of T. colubriformis and H. placei was studied. The cuticle of both nematodes was composed of (1) a membrane-bound layer, (2) external cortex, (3) inner cortex, (4) matrix, (5)–(7) three fibre layers, and (8) a basal lamella. Pear-shaped bodies embedded in the matrix layer in H. placei occur, along the length of the animal, once per annulus. A thin hypodermis enlarged to form lateral, dorsal and ventral cords which contained hypodermal cells, nerves and excretory canals. The muscle cells were large and multilobular. Two sizes of myofilaments, present in the contractile part of the cell, conformed to the filament arrangement of obliquely striated muscle. The mouth, buccal cavity and oesophagus of the alimentary tract were lined with cuticle. The oesophagus contained gland cells, muscle cells and apical cells. The lumen of the intestinal cells of both species had a well-developed microvillar border. In H. placei each microvillus was encircled by a ring of apparently spirally orientated vesicles or tubules.

The authors are grateful to Dr D. W. Brocklesby for his help and advice. They would also like to thank Dr D. L. Lee and Dr W. G. MacMillan for helpful discussions.

Type
Research Article
Information
Parasitology , Volume 64 , Issue 2 , April 1972 , pp. 173 - 179
Copyright
Copyright © Cambridge University Press 1972

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References

REFERENCES

Bird, A. F. & Deutsch, K. (1957). The structure of the cuticle of Ascaris lumbricoides var. suis. Parasitology 47, 319–27.CrossRefGoogle ScholarPubMed
Crofton, H. D. (1966). Nematodes. London: Hutchinson University Library.Google Scholar
Eckert, J. & Schwarz, R. (1965). Zur Struktur der Cuticula, invasionsfahiger Larven einiger Nematoden. Zeitschrift für Parasitenkunde 26, 116–42.CrossRefGoogle Scholar
Harness, E., Fitzsimmons, W. M. & Sellwood, S. A. (1970). Experimental Haemonchus placei infection in calves. Journal of Comparative Pathology 80, 173–9.CrossRefGoogle ScholarPubMed
Harris, J. E. & Crofton, H. D. (1957). Structure and function in the nematodes: internal pressure and cuticular structure in Ascaris. Journal of Experimental Biology 34, 116–30.CrossRefGoogle Scholar
Jamuar, M. P. (1966). Electron microscope studies on the body wall of the nematode Nippostrongylus brasiliensis. Journal of Parasitology 52, 209–32.CrossRefGoogle Scholar
Lee, D. L. (1965). The cuticle of adult Nippostrongylus brasiliensis. Parasitology 55, 173–81.CrossRefGoogle ScholarPubMed
Lee, D. L. (1970). The fine structure of the excretory system in adult Nippostrongylus brasiliensis (Nematoda) and a suggested function for the ‘excretory glands’. Tissue and Cell 2, 225–31.CrossRefGoogle Scholar
Millonig, G. (1961). Advantage of a phosphate buffer for osmium tetroxide solutions in fixation. Journal of Applied Physics, 32, 1637.Google Scholar
Roggen, D. R., Raski, D. J. & Jones, N. O. (1967). Further electron microscopic observations of Xiphinema index. Nematologica 13, 1–16.CrossRefGoogle Scholar
Rosenbluth, J. (1965). Ultrastructural organisation of obliquely striated muscle fibres in Ascaris lumbricoides. Journal of Cell Biology 25, 495–515.CrossRefGoogle ScholarPubMed
Sabatini, D. D., Bensch, K. & Barnett, R. J. (1963). Cytochemistry and electron microscopy. The presentation of cellular ultrastructure and enzymatic activity by aldehyde fixation. Journal of Cell Biology 17, 19–58.CrossRefGoogle Scholar
Smith, K. (1970). Electron microscopical observations on the body wall of the third-stage larvae of Haemonchus placei. Parasitology 60, 411–16.CrossRefGoogle ScholarPubMed
Smith, K. & Harness, E. (1971). The fine structure of the alimentary tract of third-stage larvae of Haemonchus placei and Trichostrongylus colubriformis. Parasitology 62, 355–60.CrossRefGoogle ScholarPubMed
Venable, J. H. & Coggeshall, R. (1965). A simplified lead citrate stain for use in electron microscopy. Journal of Cell Biology 25, 407–8.CrossRefGoogle ScholarPubMed
Wright, K. A. (1963). The cytology of the intestine of the parasitic nematode Capillaria hepatica (Bancroft, 1893). Journal of Ultrastructural Research 9, 143–55.CrossRefGoogle Scholar
Wright, K. A. & Jones, N. O. (1965). Some techniques for the orientation and embedding of nematodes for electron microscopy. Nematologica 11, 125–30.Google Scholar