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Electron microscope study of the blood stages of Plasmodium tropiduri, a lizard malaria parasite*

Published online by Cambridge University Press:  06 April 2009

J. V. Scorza
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Extract

The study of the ultrastructure of the blood stages of P.tropiduri Aragao & Neiva, 1909 shows close similarities between this species and P.floridense, another lizard malaria parasite. The absence of a rounded structure in the merozoites of P.tropiduri, and their tendency not to project beyond the original area occupied by the schizont are the ultrastructural characteristics that differentiate the two species.

The close relationships of homology between P.tropiduri and the Plasmodium of birds are emphasized, and new evidence on their phylogenetic relationships is offered.

P.tropiduri has a mode of ingestion and digestion similar to that of P. elongatum. When the size of the cytostome, the size of the boluses produced by the phagotrophy, and the size of the digestive vacuoles are compared, they are seen to be nearly identical in the two species.

The comparative study of the structure of the cytostomes of the merozoites and the trophozoites, and of their relationships with the membranes that limit the parasites, gives new evidence that the external membrane of the trophozoites schizonts, and gametocytes is derived from the host cell.

An exflagellatory apparatus is described from the microgametocytes of P.tropiduri, and its relationships with similar structures described from the gametocytes of avian Plasmodium and from Coccidia are discussed.

Type
Research Article
Information
Parasitology , Volume 63 , Issue 1 , August 1971 , pp. 1 - 20
Copyright
Copyright © Cambridge University Press 1971

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References

REFERENCES

Aikawa, M. (1966). The fine structure of the erythrocytic stages of three avian malarial parasites, Plasmodium fallax, P. lophurae and P. cathemerium. American Journal of Tropical Medicine and Hygiene 15, 449–71.CrossRefGoogle ScholarPubMed
Aikawa, M., Hepler, P. K., Huff, C. G. & Sprinz, H. (1966 a). The feeding mechanism of avian malarial parasites. Journal of Cell Biology 28, 355–73.CrossRefGoogle ScholarPubMed
Aikawa, M., Huff, C. G. & Sprinz, H. (1966 b). Comparative feeding mechanism of avian and primate malarial parasites. Military Medicine 131 (Suppl.), 869–983.Google Scholar
Aikawa, M., Huff, C. G. & Sprinz, H. (1967). Fine structure of the asexual stages of Plasmodium elongatum. Journal of Cell Biology 34, 229–49.Google Scholar
Aikawa, M., Huff, C. G. & Sprinz, H. (1968). Exoerythrocytic stages of Plasmodium gallinaceum in chick-embryo liver as observed electromicroscopically. American Journal of Tropical Medicine and Hygiene 17, 156–69.CrossRefGoogle Scholar
Aikawa, M. & Jordan, H. B. (1968). Fine structure of a reptilian malarial parasite. Journal of Parasitology 54, 1023–33.Google Scholar
Aikawa, M., Cook, R. T., Sakoda, J. M. & Sprinz, H. (1969). Fine structure of erythrocytic stages of Plasmodium knowlesi. Zeischrift für Zellforschung und mikroskopische Anatomie 100, 271–84.Google Scholar
Aikawa, M., Huff, C. G. & Strome, C. P. A. (1970). Morphological study of microgametogenesis of Leucocytozoon simondi. Journal of Ultrastructure Research 32, 43–68.CrossRefGoogle ScholarPubMed
Aragao, H. de B. & Neiva, A. (1909). A contribution to the study of the intraglobular parasites of the lizards. Two new species of Plasmodium. Pl. diploglossi, n.sp. and Pl. tropiduri, n.sp. Memorias do Instituto Oswaldo Cruz 1, 44–50.CrossRefGoogle Scholar
Bishop, A. & McConnachie, E. W. (1956). A study of the factors affecting the emergence of the gametocytes of Plasmodium gallinaceum from the erythrocytes and the exflagellation of the male gametocytes. Parasitology 46, 192–215.Google Scholar
Boisson, C. & Mattei, X. (1965). Sur la spermiogenese de Python sebae (Gmelin) etudiée au microscope electronique. Compte rendu des séances de la Societé de biologie 159, 1192–4.Google Scholar
Carini, A. (1941). Rectificacoes necessarias. Archivos de biologia (Sao Paulo) 25 (238), 205.Google Scholar
Caulfield, J. B. (1957). Effects of varying the vehicle for OsO4 in tissue fixation. Journal of Biophysical and Biochemical Cytology 3, 827.CrossRefGoogle ScholarPubMed
Duncan, D., Street, J., Julian, S. R. & Micks, D. O. (1959). Electron microscopic observations of the gametocytes of a malarial parasite (Plasmodium cathemerium). Texas Reports on Biology and Medicine 17, 314–22.Google Scholar
Garnham, P. C. C. (1969). The structure and function of the cytostome (= micropyle) in the Sporozoa. Progress in Protozoology, 3rd International Congress of Protozoology (Leningrad) p.8.Google Scholar
Garnham, P. C. C., Bird, R. G., Baker, J. R. & Bray, R. S. (1961). Electron microscope study of motile stages of malaria parasites. II. The fine structure of sporozoites of Laverania (= Plasmodium) falciparum. Transactions of the Royal Society of Tropical Medicine and Hygiene 55, 98–102.CrossRefGoogle Scholar
Garnham, P. C. C., Bird, R. G. & Baker, J. R. (1967). Electron microscope studies of motile stages of malaria parasites. V. Exflagellation in Plasmodium, Hepatocystis and Leucocytozoon. Transactions of the Royal Society of Tropical Medicine and Hygiene 61, 58–68.Google Scholar
Garnham, P. C. C., Bird, R. G., Baker, J. R., Desser, S. S. & El-Nahal, H. M. S. (1969). Electron microscope studies on motile stages of malaria parasites. VI. The oökinete of Plasmodium berghei yoelii and its transformation into the early oöcyst. Transactions of the Royal Society of Tropical Medicine and Hygiene 63, 187–94.Google Scholar
Hammond, D. M., Scholtyseck, E. & Chobotary, B. (1969). Fine structural study of the microgametocytogenesis of Eimeria auburnensis. Zeitschrift für Parasitenkunde 33, 65–84.Google Scholar
Heller, G. (1969). Elektronenmikroskopische Untersuchungen aus Aggregata eberthi aus dem Spiraldarm von Sepia officianalis (Sporozoa, Coccidia). Zeitschrift für Parasitenkunde 33, 44–64.Google Scholar
Hepler, P. K., Huff, C. G. & Sprinz, H. (1966). The fine structure of the exoerythrocytic stages of Plasmodium fallax. Journal of Cell Biology 30, 333–59.Google Scholar
Killick-Kendrick, R. & Warren, McW. (1968). Primary exoerythrocytic schizonts of a mammalian Plasmodium as a source of gametocytes. Nature 220, 191–2.Google Scholar
Ladda, R., Arnold, J., Martin, D. & Luehrs, F. (1965). Electron microscopy of Plasmodium berghei. I. On the migration of trophozoites from infected erythrocytes in the rat. Transactions of the Royal Society of Tropical Medicine and Hygiene 59, 420–2.Google Scholar
Lainson, R. & Shaw, J. J. (1969). New host records for Plasmodium diploglossi, P. tropiduri Aragao & Neiva, 1909, and P. cnemidophori Carini, 1941. Parasitology 59, 163–70.CrossRefGoogle ScholarPubMed
McLaren, D. (1969). Observations on the fine structural changes associated with schizogony in Eimeria tenella. Parasitology 59, 563–74.Google Scholar
Renaud, F. L. & Swift, L. (1964). The development of basal bodies and flagella in Allomyces arbusculus. Journal of Cell Biology 23, 339–54.Google Scholar
Reynolds, E. S. (1963). The use of lead citrate as an electron dense stain in electron microscopy. Journal of Cell Biology 17, 208–12.Google Scholar
Rudzinska, M. A. (1969). The fine structure of malaria parasites. International Review of Cytology 25, 161–99.Google Scholar
Rudzinska, M. A. & Trager, W. (1961). The role of the cytoplasm during reproduction in a malarial parasite (Plasmodium lophurae) as revealed by electron microscopy. Journal of Protozoology 8, 307–22.CrossRefGoogle Scholar
Runzinska, M. A., Trager, W. & Bray, R. S. (1965). Pinocytotic uptake and the digestion of haemoglobin in malaria parasites. Journal of protozoology 12, 563–76.Google Scholar
Runzinska, M. A. & Trager, W. (1968). The fine structure of trophozoites and gametocytes in Plasmodium coatneyi. Journal of Protozoology 15, 73–88.Google Scholar
Scalzi, A. A. & Bahr, G. F. (1968). An electron microscopic examination of erythrocytic stages of two rodent malarial parasites, Plasmodium chabaudi and Plasmodium vinckei. Journal of Ultrastructure Research 24, 116–33.Google Scholar
Scholtyseck, E., Mehlhorn, H. & Friedhoff, K. (1970). The fine structure of the conoid of Sporozoa and related organisms. Zeitschrift für Parasitenkunde 34, 68–94.Google Scholar
Scorza, J. V. (1971). Unpublished observations.Google Scholar
Vickerman, K. & Cox, F. E. G. (1967). Merozoite formation in the erythrocytic stages of the malaria parasites Plasmodium vinckei. Transactions of the Royal Society of Tropical Medicine and Hygiene 61, 303–12.Google Scholar
Watson, M. L. (1958). Staining of tissue sections for electron microscopy with heavy metals. Journal of Biophysics and Biochemical Cytology 4, 475–8.Google Scholar