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Environmental contamination with parasites in selected rural farms in the Philippines: impacts of farming practices on leafy greens food safety

Published online by Cambridge University Press:  06 December 2021

Vachel Gay V. Paller Open the ORCID record for Vachel Gay V. Paller [Opens in a new window] ,
Constance Aurelle Macalinao-Ramirez  and
Modesto Z. Bandal Jr.
Vachel Gay V. Paller*
Affiliation:
Parasitology Research Laboratory, Animal Biology Division, Institute of Biological Sciences, University of the Philippines Los Baňos, College 4031, Laguna, Philippines
Constance Aurelle Macalinao-Ramirez
Affiliation:
Parasitology Research Laboratory, Animal Biology Division, Institute of Biological Sciences, University of the Philippines Los Baňos, College 4031, Laguna, Philippines
Modesto Z. Bandal Jr.
Affiliation:
Parasitology Research Laboratory, Animal Biology Division, Institute of Biological Sciences, University of the Philippines Los Baňos, College 4031, Laguna, Philippines
*
Author for correspondence: Vachel Gay V. Paller, E-mail: vvpaller@up.edu.ph
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Abstract

Fresh vegetables are an important part of a healthy diet, however, they can also be agents of transmission of intestinal parasites. This study aimed to evaluate the presence of parasite contamination in vegetable farms as a source of transmission in selected rural communities in Laguna, Philippines. A total of 168 vegetable, 55 soil and 15 water samples collected from four selected farms and a reference farm were processed through various standard parasitological techniques. Of these, 17.3% of vegetables, 47.3% soil and 73.3% water samples were found contaminated with parasites’ eggs or cysts. Interestingly, leafy vegetables, such as lettuce, were found to be more contaminated. Results showed that strongylids/hookworms (egg/larva) showed the highest prevalence in farm soil (38.2%) and vegetable (13.1%); other helminth parasite eggs were also recovered such as Toxocara sp., Ascaris sp., Trichiuris, Trichostrongylus sp. and protozoan cysts of Balantidium coli. Cryptosporidium sp. oocysts of and Giardia sp. cysts were observed in all water samples in the farms. Furthermore, results revealed that some farming practices such as the use of improperly treated animals manure as fertilizers, unhygienic practice of farmers and sanitation issues were factors that contribute to parasite contamination in the farms. These findings have implications on food safety in poor-resource communities posing public health risks. Recommendations were discussed in the study for the control and prevention of parasite contamination at the farm level.

Keywords

Farming practicesfood safetyfoodborne parasitespublic healthzoonosis
Type
Research Article
Information
Parasitology , Volume 149 , Issue 4 , April 2022 , pp. 482 - 489
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Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press

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References

Adamu, NB, Adamu, JY and Mohammed, D (2012) Prevalence of helminth parasites found on vegetables sold in Maiduguri, Northeastern Nigeria. Food Control 25, 23–26.CrossRefGoogle Scholar
Adanir, R and Tasci, F (2013) Prevalence of helminth eggs in raw vegetables consumed in Burdur, Turkey. Food Control 31, 482–484.CrossRefGoogle Scholar
Adekeye, TA, Thompson, E and Awobode, HO (2016) Environmental contamination and public health risk of soil parasites in Ibadan South East Local Government Area, Nigeria. Zoology and Ecology 26, 150–157.CrossRefGoogle Scholar
Adenusi, AA, Abimbola, WA and Adewoga, TOS (2015) Human intestinal helminth contamination in pre-washed, fresh vegetables for sale in major markets in Ogun State, Southwest Nigeria. Food Control 50, 843–849.CrossRefGoogle Scholar
Amoah, ID, Adegoke, AA and Stenström, TA (2018) Soil-transmitted helminth infections associated with wastewater and sludge reuse: a review of current evidence. Tropical Medicine and International Health 23, 692–703.CrossRefGoogle ScholarPubMed
Baneth, G, Thamsborg, SM, Otranto, D, Guillot, J, Blaga, R, Deplazes, P and Solano-Gallego, L (2016) Major parasitic zoonoses associated with dogs and cats in Europe. Journal of Comparative Pathology 155, 54–74.CrossRefGoogle ScholarPubMed
Betson, M, Nejsum, P, Bendall, RP, Deb, RM and Stothard, JR (2014) Molecular epidemiology of ascariasis: a global perspective on the transmission dynamics of Ascaris in people and pigs. Journal of Infectious Diseases 210, 932–941.CrossRefGoogle ScholarPubMed
Betson, M, Alonte, AJI, Ancog, RC, Aquino, AO, Belizario, VY, Bordado, AD, Clark, JE, Corales, MCG, Dacuma, MGB, Divina, BP, Dixon, MA, Gourley, SA, Jimenez, JD, Jones, BP, Manalo, SMP, Prada, JM, Van Vliet, AHM, Whatley, KCL and Paller, VGV (2020) Zoonotic transmission of intestinal helminths in Southeast Asia: implications for control and elimination. Advances in Parasitology 108, 47–131.CrossRefGoogle ScholarPubMed
Blaszkowska, J, Kurnatowski, P and Damiecka, P (2011) Contamination of the soil by eggs of geohelminths in rural areas of Lodz district (Poland). Helminthologia 2, 67–76.CrossRefGoogle Scholar
Castillo, DC and Paller, VGV (2018) Occurrence of Angiostrongylus cantonensis in rodents from the rice granary of the Philippines and associated risk factors for zoonotic transmission. Journal of Parasitic Diseases 42, 350–356.CrossRefGoogle ScholarPubMed
Ćirković, V, Uzelac, A, Klun, I and Djurković-Djaković, O (2017) Protozoa as contaminants of surface water – methods of detection. Water Resources Management 7, 45–51.Google Scholar
De Leon, WU, Monzon, RB, Aganon, AA, Arceo, RE, Ignacio, EJ and Santos, G (1992) Parasitic contamination of selected vegetables sold in metropolitan Manila, Philippines. Southeast Asian Journal of Tropical Medicine and Hygiene 23, 162–164.Google ScholarPubMed
Dorny, P, Praet, N, Deckers, N and Gabriel, S (2009) Emerging food-borne parasites. Veterinary Parasitology 163, 196–206.CrossRefGoogle ScholarPubMed
Eraky, MA, Rashed, SM, Nasr, ME, El-Hamshary, AMS and El-Ghannam, AS (2014) Parasitic contamination of commonly consumed fresh leafy vegetables in Benha, Egypt. Journal of Parasitology Research 2014, 613960.CrossRefGoogle ScholarPubMed
Ferreira, FP, Caldart, ET, Freire, RI, Mitsuka-Breganó, R, De Freitas, FM, Miura, AC, Mareze, M, Martins, F, Urbano, MR, Seifert, AL and Navarro, IT (2018) The effect of water source and soil supplementation on parasite contamination in organic vegetable gardens. Brazilian Journal of Veterinary Parasitology 27, 327–337.Google ScholarPubMed
Gupta, N, Khan, DK and Santra, SC (2009) Prevalence of intestinal helminth eggs on vegetables grown in wastewater-irrigated areas of Titagarh, West Bengal, India. Food Control 20, 942–945.CrossRefGoogle Scholar
Gyawali, P, Khanal, S and Soares Magalhaes, RJ (2013) Helminth infections in an indigenous community of Nepal: the role of individual and household socio-economic factors. Global Journal of Medical Research Diseases 13, 33–39.Google Scholar
Hassan, AA, Oyebamiji, DA and Idowu, OF (2017) Spatial patterns of soil-transmitted helminths in soil environment around Ibadan, an endemic area in south-west Nigeria. Nigerian Journal of Parasitology 38, 179–184.CrossRefGoogle Scholar
Horiuchi, S and Uga, S (2016) Modified flotation method, an effective technique for recovering helminth eggs in soil. Parasitology International 65, 576–579.CrossRefGoogle ScholarPubMed
Horiuchi, S, Paller, VGV and Uga, S (2013) Soil contamination by parasite eggs in rural village in the Philippines. Tropical Biomedicine 30, 495–503.Google ScholarPubMed
Jourdan, PM, Lamberton, P, Fenwick, A and Addiss, DG (2018) Soil-transmitted helminth infections. Lancet (London, England) 391, 252–265.CrossRefGoogle ScholarPubMed
Lo, CLC, Fernandez, DAP, de Luna, MCT, de Guia, APO and Paller, VGV (2021) Diet, parasites, and other pathogens of Sunda leopard cats (Prionailurus javanensis Desmarest 1816) in Aborlan, Palawan Island, Philippines. Journal of Parasitic Diseases 45, 627–633.CrossRefGoogle ScholarPubMed
Luz, JGG, Barbosa, MV, De Carvalho, AG, Resende, SD, Dias, JVL and Martins, HR (2017) Contamination by intestinal parasites in vegetables marketed in an area of Jequitinhonha Valley, Minas Gerais, Brazil. Brazilian Journal of Nutrition (Revista de Nutricao) 30, 127–136.Google Scholar
Maffei, DF, Batalha, EY, Landgraf, M, Schaffner, DW and Franco, BDGM (2016) Microbiology of organic and conventionally grown fresh produce. Brazilian Journal of Microbiology 47, 99–105.CrossRefGoogle ScholarPubMed
Maikai, BV, Baba-Onoja, EBT and Elisha, IA (2013) Contamination of raw vegetables with cryptosporidium oocysts in markets within Zaria Metropolis, Kaduna State, Nigeria. Food Control 31, 45–48.CrossRefGoogle Scholar
Ordoňez, K, Paller, VGV, Goh, XT and Lim, Y (2016) Parasite contamination of vegetables from selected organic and conventional farms in Laguna and Benguet Province, Philippines, Pertanika. Journal of Tropical Agricultural Sciences 41, 1–20.Google Scholar
Owada, K, Lau, CL, Leonardo, L, Clements, ACA, Yakob, L, Nielsen, M, Carabin, H and Soares Magalhães, RJ (2018) Spatial distribution and populations at risk of A. lumbricoides and T. trichiura co-infections and infection intensity classes: an ecological study. Parasite Vector 11, 1–13.CrossRefGoogle Scholar
Paller, VGV and Babia-Abion, S (2019) Soil-transmitted helminth (STH) eggs contaminating soils in selected organic and conventional farms in the Philippines. Parasite Epidemiology and Control 7, e00119.CrossRefGoogle ScholarPubMed
Paller, VGV and De Chavez, ERC (2014) Toxocara (Nematoda: Ascaridida) and other soil-transmitted helminth eggs contaminating soils in selected urban and rural areas in the Philippines. The Scientific World Journal 2014, 386232.CrossRefGoogle Scholar
Soares Magalhães, RJ, Salamat, MS, Leonardo, L, Gray, DJ, Carabin, H, Halton, K, McManus, DP, Williams, GM, Rivera, P, Saniel, O, Hernandez, L, Yakob, L, McGarvey, ST and Clementis, ACA (2015) Mapping the risk of soil-transmitted helminthic infections in the Philippines. PLOS Neglect Tropical Diseases 9, 1–15.CrossRefGoogle ScholarPubMed
Su, GLS, Mariano, CMR, Matti, NSA and Ramos, GB (2012) Assessing parasitic infestation of vegetables in selected markets in Metro Manila, Philippines. Asian Pacific Journal of Tropical Diseases 2, 51–54.CrossRefGoogle Scholar
Torgerson, PR and Macpherson, CN (2011) The socioeconomic burden of parasitic zoonoses: global trends. Veterinary Parasitology 182, 79–95.CrossRefGoogle ScholarPubMed
Tudor, P (2015) Soil contamination with canine intestinal parasites eggs in the parks and shelter dogs from Bucharest area. Agriculture and Agricultural Science Procedia 6, 387–391.CrossRefGoogle Scholar
Uga, S, Hoa, NT, Noda, S, Moji, K, Cong, LD, Aoki, Y, Rai, SK and Fujimaki, Y (2009) Parasite egg contamination of vegetables from a suburban market in Hanoi, Vietnam. Nepal Medical College Journal 11, 75–78.Google ScholarPubMed
USEPA (2001) Cryptosporidium: Drinking Water Health Advisory. Washington, D.C., USA: United States Environmental Protection Agency, pp. 1–31.Google Scholar
Vizon, KCC, Battad, ZG and Castillo, DSC (2019) Contamination of food-borne parasites from green-leafy vegetables sold in public markets of San Jose City, Nueva Ecija, Philippines. Journal of Parasitic Diseases 43, 651–657.CrossRefGoogle Scholar
Woldetsadik, D, Drechsel, P, Kerait, B, Itanna, F, Erko, B and Gebrekidan, H (2017) Microbiological quality of lettuce (Lactuca sativa) irrigated with wastewater in Addis Ababa, Ethiopia and effect of green salads washing methods. International Journal of Food Contamination 4, 3.CrossRefGoogle Scholar
Ybañez, RHD, Resuelo, KGJ, Kintanar, APM and Ybañez, AP (2018) Detection of gastrointestinal parasites in small-scale poultry layer farms in Leyte, Philippines. Veterinary World 11, 1587–1591.CrossRefGoogle ScholarPubMed
Zibaei, M, Bahadory, S, Cardillo, N and Khatami, AR (2017) Soil contamination with eggs of Toxocara species in public parks of Karaj, Iran. International Journal of Enteric Pathogens 5, 45–48.CrossRefGoogle Scholar