Hostname: page-component-7bb8b95d7b-s9k8s Total loading time: 0 Render date: 2024-09-17T11:49:24.087Z Has data issue: false hasContentIssue false
  • English
  • Français

Functional antigens of Trichuris muris released during in vitro maintenance: their immunogenicity and partial purification

Published online by Cambridge University Press:  06 April 2009

S. N. Jenkins  and
D. Wakelin
S. N. Jenkins
Affiliation:
Wellcome Laboratories for Experimental Parasitology, University of Glasgow, Bearsden Road, Bearsden, Glasgow G61 1QH
D. Wakelin
Affiliation:
Wellcome Laboratories for Experimental Parasitology, University of Glasgow, Bearsden Road, Bearsden, Glasgow G61 1QH
Get access Rights & Permissions [Opens in a new window]

Summary

The immunogenicity of the secretory exo-antigen (EXA) originating in the stichosome of Trichuris muris has been investigated. High levels of immunity to challenge were produced using EXA (100 μg) by subcutaneous administration. Vaccination with a range of doses of EXA in Freunds' Incomplete Adjuvant (FIA) showed a dose response; 1 μg of EXA reduced the worm burden by 50% and doses of 10 μg or more reduced the worm burden by 80–90% at Day 9 post-infection. Worms recovered from vaccinated mice were significantly smaller than those from controls. A precipitin line identifiable with EXA was shown to immunize mice against T. muris challenge by vaccination. The protective antigen contained in EXA has been partially isolated using gel filtration, ion exchange filtration and isoelectric focusing and has been shown to be a protein of 30000 molecular weight having a pi of 6·8–7·3.

Type
Research Article
Information
Parasitology , Volume 86 , Issue 1 , February 1983 , pp. 73 - 82
Copyright
Copyright © Cambridge University Press 1983

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Clegg, J. A. & Smith, M. A. (1978). Prospects for the development of dead vaccines against helminths. Advances in Parasitology 16, 165–218.CrossRefGoogle ScholarPubMed
Despommier, D. D., Campbell, W. C. & Blair, L. S. (1977). The in vivo and in vitro analysis of immunity to Trichinella spiralis in mice and rats. Parasitology 74, 109–19.CrossRefGoogle ScholarPubMed
Despommier, D. D. & Laccetti, A. (1981). Trichinella spiralis: Proteins and antigens isolated from a large particle fraction derived from the muscle larva. Experimental Parasitology 51, 279–95.CrossRefGoogle ScholarPubMed
Despommier, D. D. & Muller, M. (1970). Functional antigens of Trichinella spiralis. Journal of Parasitology 56, 76.Google Scholar
Despommier, D. D. & Muller, M. (1976). The stichosome and its secretion granules in the mature muscle larvae of Trichinella spiralis. Journal of Parasitology 62, 775–85.CrossRefGoogle Scholar
Jenkins, S. N. & Behnke, J. M. (1977). Impairment of primary expulsion of Trichuris muris in mice concurrently infected with Nematospiroides dubius. Parasitology 75, 71–8.CrossRefGoogle ScholarPubMed
Jenkins, S. N. & Wakelin, D. (1977). The source and nature of some functional antigens of Trichuris muris. Parasitology 74, 153–61.CrossRefGoogle Scholar
Kroll, J. & Andersen, M. M. (1976). Specific antisera produced by immunisation with precipitin lines. Journal of Immunological Methods 13, 125–30.CrossRefGoogle ScholarPubMed
Kwa, B. H. & Liew, F. Y. (1977). Immunity in Taeniasis-cysticercosis I. Vaccination against T. taeniae-formis in rats using purified antigen. Journal of Experimental Medicine 146, 118–31.CrossRefGoogle Scholar
Miller, T. E., Mackaness, G. B. & Lagrange, P. H. (1973). Immunopotentiation with BCG II. Modulation of the response to sheep red blood cells. Journal of the National Cancer Institute 51, 1669–76.CrossRefGoogle ScholarPubMed
Phillip, M., Parkhouse, R. M. E. & Ogilvie, B. M. (1980). Changing proteins on the surface of a parasitic nematode. Nature, London 287, 538–40.CrossRefGoogle Scholar
Phillip, M., Parkhouse, R. M. E. & Ogilvie, B. M. (1981). Molecular basis for stage specificity of the primary antibody response to the surface of Trichinella spiralis. In Trichinellosis (ed. Kim, C. W., Ruitenberg, E. J. and Teppema, J. S.), pp. 59–63. England: Reedbooks.Google Scholar
Rhodes, M. B., Nayak, D. P., Kelly, G. W. & Marsh, C. L. (1965). Studies in helminth enzymology. IV. Immune responses to malic dehydrogenase from Ascaris suum. Experimental Parasitology 16, 373–81.CrossRefGoogle ScholarPubMed
Rothwell, T. L. W. & Merritt, G. C. (1975). Vaccination against the nematode Trichostron-gylus colubriformis. II. Attempts to protect guinea-pigs with worm acetylcholinesterase. International Journal for Parasitology 5, 453–60.CrossRefGoogle ScholarPubMed
Sokal, R. R. & Rohlf, F. J. (1969). Biometry. San Francisco: W. H. Freeman & Co.Google Scholar
Stromberg, B. E. (1979). The isolation and partial characterisation of a protective antigen from developing larvae of Ascaris suum. International Journal for Parasitology 9, 307–12.CrossRefGoogle ScholarPubMed
Wakelin, D. (1967). Acquired immunity to Trichuris muris in the albino laboratory mouse. Parasitology 57, 515–24.CrossRefGoogle ScholarPubMed
Wakelin, D. & Sblby, G. R. (1973). Functional antigens of Trichuris muris. The stimulation of immunity by vaccination of mice with somatic antigen preparations. International Journal for Parasitology 3, 711–15.CrossRefGoogle ScholarPubMed