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Immunocytochemical demonstration of neuropeptides in the nervous system of the liver fluke, Fasciola hepatica (Trematoda, Digenea)

Published online by Cambridge University Press:  06 April 2009

R. M. Magee ,
I. Fairweather ,
C. F. Johnston ,
D. W. Halton  and
C. Shaw
R. M. Magee
Affiliation:
1Department of Biology
I. Fairweather
Affiliation:
1Department of Biology
C. F. Johnston
Affiliation:
2Department of Medicine, The Queen's University, Belfast BT7 1NN
D. W. Halton
Affiliation:
1Department of Biology
C. Shaw
Affiliation:
2Department of Medicine, The Queen's University, Belfast BT7 1NN
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Summary

The localization and distribution of neuropeptides in the nervous system of the liver fluke, Fasciola hepatica at different stages in the development of the adult fluke have been determined by an indirect immunofluorescence technique, using antisera to 19 vertebrate peptides and the invertebrate neuropeptide, FMRFamide. Positive immunoreactivity was obtained with antisera to pancreatic polypeptide (PP), peptide tyrosine tyrosine (PYY), substance P (SP) and FMRFamide. Cell bodies and nerve fibres immunoreactive to the 4 peptides are present in the anterior ganglia and the 3 pairs of longitudinal nerve cords and their commissures in the central nervous system. In the peripheral nervous system, immunoreactivity occurs in the nerve plexuses supplying the subtegumental musculature, the oral and ventral suckers, and the muscular lining of the male and female reproductive ducts, including the ootype, uterus, cirrus pouch and gonopore. Cells displaying immunoreactivity to PYY and FMRFamide lie amongst the Mehlis' gland cells that surround the ootype. Processes from these cells extend into the wall of the ootype. One group of PP-immunoreactive cells occurs at the junction of the vitelline and ovovitelline ducts, whilst another group is situated at the entrance to the uterus from the ootype. The results are discussed in relation to the possible roles of the peptides in the neurophysiology and egg production of the fluke.

Keywords

Fasciola hepaticatrematode neuropeptidesimmunocytochemistrypancreatic polypeptidepeptide tyrosine tyrosinesubstance P FMRFamide
Type
Research Article
Information
Parasitology , Volume 98 , Issue 2 , April 1989 , pp. 227 - 238
Copyright
Copyright © Cambridge University Press 1989

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