Hostname: page-component-77c89778f8-cnmwb Total loading time: 0 Render date: 2024-07-16T09:43:38.194Z Has data issue: false hasContentIssue false
  • English
  • Français

Merozoite vaccination of rhesus monkeys against plasmodium knowlesi malaria; immunity to sporozoite (mosquito-transmitted) challenge

Published online by Cambridge University Press:  06 April 2009

W. H. G. Richards ,
G. H. Mitchell ,
G. A. Butcher  and
S. Cohen
W. H. G. Richards
Affiliation:
Wellcome Research Loboratories, Beckenham, Kent
G. H. Mitchell
Affiliation:
Department of Chemical Pathology, Guy's Hospital Medical School, London, SE1 9RT
G. A. Butcher
Affiliation:
Department of Chemical Pathology, Guy's Hospital Medical School, London, SE1 9RT
S. Cohen
Affiliation:
Department of Chemical Pathology, Guy's Hospital Medical School, London, SE1 9RT
Get access Rights & Permissions [Opens in a new window]

Extract

Five normal rhesus monkeys were infected with Plasmodium knowlesi sporozoites (A-strain); two developed rapidly fatal malaria and three chrinic relapsing infections. Vaccination with P. knowlesi (W-strain) merozoites (unmodified or formol-treated and freeze-dried) in Freund's complete adjuvant (FCA) did not inhibit pre-erythrocytic parasite development after challenge with A-strain sporozoites. However, the subsequent blood-stage infection was terminated in nine out of ten vaccinated monkeys even though the challenge strain was different form that used for vaccination. The degree of parasitaemia (0·01–0·70 %) and brevity of infection (1–12 days) in six animals vaccinated with untreated merzoites was similar to that observed after direct challenge with blood-stage parasites. Monkeys were equally resistant to sporozoite challenge given as the post-vaccination infection or administered 6 months after blood challenge. These results are discussed in relation to the development of a human malaria vaccine.

Type
Research Article
Information
Parasitology , Volume 74 , Issue 2 , April 1977 , pp. 191 - 198
Copyright
Copyright © Cambridge University Press 1977

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Alving, A. S., Craige, B., Pullman, T. N., Whorton, C. M., Jones, R. & Elchelberger, L. (1948). Procedures used at Srateville Penitentiary for the testing of potential antimalarial agents. Journal of Clinical Investigation 27, (3, Part 2) 2–5.CrossRefGoogle ScholarPubMed
Brown, I. N. (1969). Immunological aspects of malaria infection. Advances in Immunology 11, 267–349.CrossRefGoogle ScholarPubMed
Brown, K. N. (1976). Resistance to malaria. In Immunology of Parasitic Infections (ed. Cohen, S., Sadun, E. H.), p. 268. Oxford: Blackwell Scientific Publications.Google ScholarPubMed
Brown, K. N. & Brown, I. N. (1965). Immunity to malaria: antigenic variation in chronic infections of Plasmodium knowlesi. Nature, London 208, 1286–8.CrossRefGoogle ScholarPubMed
Butcher, G. A. & Cohen, S. (1972). Antigenic variation and protective immunity in plasmodium knowlesi malaria. Immunology 23, 503–21.Google ScholarPubMed
Butcher, G. A., Mitchell, G. H. & Cohen, S. (1973). Mechanism of host specificity in malarial infection. Nature, London 244, 40–2.CrossRefGoogle ScholarPubMed
Carter, R. & Chen, D. H. (1976). Malaria transmission blocked by immunisation with gametes of the malaria parasite. Nature, London 263, 57–60.CrossRefGoogle ScholarPubMed
Clyde, D. F., McCarthy, V. C., Miller, R. M. & Woodward, E. E. (1975). Immunisation of man agiainst falciparum and vivax malaria by the use of attenuated sporozoites. American Journal of Tropical Medicine and Hygiene 24, 397–401.CrossRefGoogle ScholarPubMed
Cohen, S. (1975). Immunoprophylaxis of protozoal diseases. In Clinical Aspects of Immunology, 3rd ed. (ed. Gell, R. G. H., Coombs, R. R. A., Lachmenn, P. J.). p. 1649. Oxford: Blackwell Scientific Publications.Google Scholar
Cohen, S., Butcher, G. A. & Mitchell, G. H. (1974). Mechanisms of immunity to malaria. Bulletin of the Wold Helth Organization 50, 251–7.Google ScholarPubMed
Dennis, E. D., Mitchell, G. H., Butcher, G. A. & Cohen, S. (1975). In vitro isolation of Plasmodium knowlesi merozoites using polycarbonate sieves. Parasitology 71, 475–81.CrossRefGoogle ScholarPubMed
Kramerm, L. D. & Vanderberg, J. P. (1975). Intramuscular immunisation of mice with irradiated Plasmidium berghei sporozoites. Echancement of protection with albumen. American Journal of Tropical Medical and Hygiene 24, 913.CrossRefGoogle Scholar
Lancet (1975). 2, p. 15. Editorial. Epitaph for globle malaria eradication?.Google Scholar
Miller, L. H., Dvorak, J. A., Shiroishi, T. & Durocher, J. R. (1973). Influence of erythrocyte membrane components on malaria invasion. Journal of Experimental Medicine 138, 1597–1601.CrossRefGoogle Scholar
Mitchell, G. H., Butcher, G. A. & Cohen, S. (1973). Isolation of blood-stage merozoites from Plasmodium knowlesi malaria. Internarional Journal for Parasitology 3, 443–5.CrossRefGoogle ScholarPubMed
Mitchell, G. H., Butcher, G. A. & Cohen, S. (1975). Merozoite vaccination against Plasmodium knowlesi malaria. Immunology 29, 397–407.Google ScholarPubMed
Mulligan, H. W., Russell, P. F. & Mohan, B. N. (1941). Active immunisation of fowls against Plasmodium gallinaceum by injection of killed homologous sporozoites. Journal of the Malaria Institute of India 4, 25–34.Google Scholar
Nussenzweig, R. S. & Chen, D. (1974). The antibody response to sporozoites of simian and human malaria parasites: its stage and species specificity and strain cross-reactivity Bulletin of the World Health Organization 50, 239–4.Google Scholar
Richards, W. H. G. (1966). Active immunisation of chicks against Plasmodium gallinaceum by inactivated homologous sporozoites and blood parasites. Nature, Londan 212, 1492–4.CrossRefGoogle Scholar
Spitalny, G.L. & Nussenzweig, R. S (1972) Effect of various riutes of immunisation and method of parasite attenmation on the development of protection against sporozoite induced rodent malaria. Proceedings of the Helminthological Society of Washington 39, 506–14.Google Scholar
Trager, W. & Jensen, J. B. (1976). Human malaria parasites in continuous culture. Science 193, 673–5.CrossRefGoogle ScholarPubMed
World Health Organization (1975). Developmants in malaria immunology. Technical Report, no. 579.Google Scholar