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A new method for the partial purification of leishmania amastigotes from cutaneous lesions

Published online by Cambridge University Press:  06 April 2009

R. B. Infante ,
A. G. Hernandez ,
F. Riggione  and
K. Dawidowicz
R. B. Infante
Affiliation:
Facultad de Ciencias, Universidad Central de Venezuela, Apartado 10098, Caracas, Venezuela
A. G. Hernandez
Affiliation:
Facultad de Ciencias, Universidad Central de Venezuela, Apartado 10098, Caracas, Venezuela
F. Riggione
Affiliation:
Instituto Venezolano de Investigaciones Cientificas, Venezuela
K. Dawidowicz
Affiliation:
Facultad de Ciencias, Universidad Central de Venezuela, Apartado 10098, Caracas, Venezuela
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Summary

Amastigotes from Leishmania braziliensis and L. mexicana were isolated from cutaneous lesions in infected animals using the plant lectin Concanavalin A as a specific agglutination agent. Amastigotes were collected in preparations of up to 95% purity as determined by cell count. The parasites obtained by this method showed no apparent loss of viability as measured by growth rates and DNA replication, or pathogenicity as measured by routine passages in hamsters or mice.

Type
Research Article
Information
Parasitology , Volume 80 , Issue 1 , February 1980 , pp. 105 - 112
Copyright
Copyright © Cambridge University Press 1980

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