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On the possible schistosomulicidal effect of macrophage-derived lysozyme

Published online by Cambridge University Press:  06 April 2009

E. Flescher ,
Y. Keisari ,
J. Lengy  and
D. Gold
E. Flescher
Affiliation:
Division of Clinical Immunology, Department of Medicine, The University of Texas Health Science Center of San Antonio, San Antonio, Texas 78284, USA
Y. Keisari
Affiliation:
Department of Human Microbiology, Sackler School of Medicine, Tel Aviv University, Tel Aviv 69978, Israel
J. Lengy
Affiliation:
Department of Human Microbiology, Sackler School of Medicine, Tel Aviv University, Tel Aviv 69978, Israel
D. Gold
Affiliation:
Department of Human Microbiology, Sackler School of Medicine, Tel Aviv University, Tel Aviv 69978, Israel
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Extract

Lysozyme secretion from macrophages of Schistosoma mansoni-infected mice was time dependent, rising significantly from the 8th week post-infection, the macrophages thereafter exhibiting very high levels (> 90%) of schistosomulicidal activity. Despite the ability of lysozyme to kill schistosomula in vitro, the concentrations required for such killing were several hundred-fold to several thousand-fold higher than those detected in the supernatants from infected-mice macrophages cultured with or without schistosomula. An in vitro lysozyme inhibitor, N, N, N-triacetyl chitobiose, did not abrogate the cytotoxic ability of macrophages from schistosome-infected mice, but an inhibitor of arginine-dependent cytotoxicity, NG-monomethyl arginine, markedly inhibited schistosomulicidal activity. Evidently, concentrations of ambient lysozyme from macrophage cultures are too low to affect schistosomula in culture, while the main schisto-somulicidal pathway in vitro seems to be arginine dependent.

Keywords

Schistosoma mansonischistosomulalysozymemacrophagesarginine
Type
Research Article
Information
Parasitology , Volume 103 , Issue 1 , August 1991 , pp. 61 - 64
Copyright
Copyright © Cambridge University Press 1991

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References

Cheng, T. C. & Dougherty, W. J. (1989). Ultrastructural evidence for the destruction of Schistosoma mansoni sporocysts associated with elevated lysosomal enzyme levels in Biomphalaria glabrata. Journal of Parasitology 75, 928–41.CrossRefGoogle ScholarPubMed
Chipman, D. M. & Sharon, N. (1969). Mechanism of lysozyme action. Science 165, 454–65.CrossRefGoogle ScholarPubMed
Diluzio, N. R. (1979). Lysozyme, glucan-activated macrophages and neoplasia. Journal of the Reticuloendothelial Society 26, 67–81.Google Scholar
Gordon, S., Todd, J. & Cohn, Z. A. (1974). In vitro synthesis and secretion of lysozyme by mononuclear phagocytes. Journal of Experimental Medicine 139, 1228–48.CrossRefGoogle ScholarPubMed
Hibbs, J. R. Jr, Taintor, R. & Vavrin, Z. (1987). Macrophage cytotoxicity: role for L-arginine deiminase and imino nitrogen oxidation to nitrate. Science 235, 473–6.CrossRefGoogle Scholar
HsÜ, S. Y., Li, Y., HsÜ, H. F., Isacson, P. & Cheng, H. F. (1977). Schistosoma mansoni and S. japonicum: methylene blue test for the viability of schistosomula in vitro. Experimental Parasitology 41, 329–34.CrossRefGoogle ScholarPubMed
James, S. L. & Glaven, J. (1989). Macrophage cytotoxicity against schistosomula of Schistosoma mansoni involves arginine-dependent production of reactive nitrogen intermediates. Journal of Immunology 143, 4208–12.CrossRefGoogle ScholarPubMed
James, S. L., Sher, A., Lazdins, J. K. & Meltzer, M. S. (1982). Macrophages as effector cells of protective immunity in murine schistosomiasis. II. Killing of newly transformed schistosomula in vitro by macrophages activated as a consequence of Schistosoma mansoni infection. Journal of Immunology 128, 1535–40.CrossRefGoogle ScholarPubMed
Lazdins, J. K., Stein, M. J., David, J. R. & Sher, A. (1982). Schistosoma mansoni: rapid isolation and purification of schistosomula of different developmental stages by centrifugation on discontinuous density gradients of percoll. Experimental Parasitology 53, 39–44.CrossRefGoogle ScholarPubMed
McLaren, D. J. & James, S. L. (1985). Ultrastructural studies of the killing of schistosomula of Schistosoma mansoni by activated macrophages in vitro. Parasite Immunology 7, 315–31.CrossRefGoogle ScholarPubMed
McLaren, D. J., Peterson, C. G. B. & Venge, P. (1984). Schistosoma mansoni: further studies of the interaction between schistosomula and granulocyte-derived cationic proteins in vitro. Parasitology 88, 491–503.CrossRefGoogle ScholarPubMed
Malkin, R., Flescher, E., Lengy, J. & Keisari, Y. (1986). The interaction between macrophages and developmental stages of Schistosoma mansoni: effect of macrophage function modulators on the viability of S. mansoni in vivo and in vitro. Journal of Biological Response Modifiers 5, 470–80.Google ScholarPubMed
Malkin, R., Flescher, E., Lengy, J. & Keisari, Y. (1987). On the interaction between macrophages and developmental stages of Schistosoma mansoni: the cytotoxic mechanisms involved in macrophage-mediated killing of schistosomula in vitro. Immunobiology 176, 63–72.CrossRefGoogle ScholarPubMed
Perrotto, J. L., Falchuk, K. R., Pelly, R. P. & Warren, K. S. (1976). Serum lysozyme and β-glucuronidase in experimentally induced granulomatous inflammation. Annals of the New York Academy of Sciences 278, 592–8.CrossRefGoogle ScholarPubMed