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Phylogeography of Trichuris populations isolated from different Cricetidae rodents

Published online by Cambridge University Press:  20 August 2012

ROCÍO CALLEJÓN ,
MANUEL DE ROJAS ,
CARLOS FELIÚ ,
FRANCISCO BALAO ,
ÁNGELA MARRUGAL ,
HEIKKI HENTTONEN ,
DIEGO GUEVARA  and
CRISTINA CUTILLAS
ROCÍO CALLEJÓN
Affiliation:
Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Seville, Profesor García González 2, 41012 Seville, Spain
MANUEL DE ROJAS
Affiliation:
Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Seville, Profesor García González 2, 41012 Seville, Spain
CARLOS FELIÚ
Affiliation:
Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Barcelona, Barcelona, Spain
FRANCISCO BALAO
Affiliation:
Department of Plant Biology and Ecology, University of Seville, Apdo. 1095, E-41080 Seville, Spain
ÁNGELA MARRUGAL
Affiliation:
Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Seville, Profesor García González 2, 41012 Seville, Spain
HEIKKI HENTTONEN
Affiliation:
Finnish Forest Research Institute, Vantaa, Finland
DIEGO GUEVARA
Affiliation:
Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Seville, Profesor García González 2, 41012 Seville, Spain
CRISTINA CUTILLAS*
Affiliation:
Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Seville, Profesor García González 2, 41012 Seville, Spain
*
*Corresponding author: Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Seville, Prof. García González 2, 41012 Seville, Spain. Tel: +34954556773. Fax: +34954628162. E-mail: cutillas@us.es
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Summary

The phylogeography of Trichuris populations (Nematoda) collected from Cricetidae rodents (Muroidea) from different geographical regions was studied. Ribosomal DNA (Internal Transcribed Spacers 1 and 2, and mitochondrial DNA (cytochrome c- oxidase subunit 1 partial gene) have been used as molecular markers. The nuclear internal transcribed spacers (ITSs) 1 and 2 showed 2 clear-cut geographical and genetic lineages: one of the Nearctic region (Oregon), although the second was widespread throughout the Palaearctic region and appeared as a star-like structure in the minimum spanning network. The mitochondrial results revealed that T. arvicolae populations from the Palaearctic region were separated into 3 clear-cut geographical and genetic lineages: populations from Northern Europe, populations from Southern (Spain) and Eastern Europe (Croatia, Belarus, Kazahstan), and populations from Italy and France (Eastern Pyrénean Mountains). Phylogenetic analysis obtained on the basis of ITS1-5·8S-ITS2 rDNA sequences did not show a differential geographical structure; however, these markers suggest a new Trichuris species parasitizing Chionomys roberti and Cricetulus barabensis. The mitochondrial results revealed that Trichuris populations from arvicolinae rodents show signals of a post-glacial northward population expansion starting from the Pyrenees and Italy. Apparently, the Pyrenees and the Alps were not barriers to the dispersal of Trichuris populations.

Keywords

phylogeographyTrichuris arvicolaeNematodaribosomal DNAmitochondrial DNACricetidaerodents
Type
Research Article
Information
Parasitology , Volume 139 , Issue 13 , November 2012 , pp. 1795 - 1812
Copyright
Copyright © Cambridge University Press 2012

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