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Sensitivity of pooled serum testing for screening antibody of schistosomiasis japonica by IHA in a mountainous area of Yunnan, China

Published online by Cambridge University Press:  21 January 2009

X. M. JIA ,
H. SRIPLUNG ,
V. CHONGSUVIVATWONG  and
A. GEATER
X. M. JIA*
Affiliation:
Department of Parasitology, Faculty of Basic Medicine, Kunming Medical University, Kunming, 650031, China Epidemiology Unit, Faculty of Medicine, Prince of Songkla University, Hat Yai, Songkhla90110, Thailand
H. SRIPLUNG
Affiliation:
Epidemiology Unit, Faculty of Medicine, Prince of Songkla University, Hat Yai, Songkhla90110, Thailand
V. CHONGSUVIVATWONG
Affiliation:
Epidemiology Unit, Faculty of Medicine, Prince of Songkla University, Hat Yai, Songkhla90110, Thailand
A. GEATER
Affiliation:
Epidemiology Unit, Faculty of Medicine, Prince of Songkla University, Hat Yai, Songkhla90110, Thailand
*
*Corresponding author: Epidemiology Unit, Faculty of Medicine, Prince of Songkla University, Hat Yai, Songkhla90110, Thailand. Tel: +66 74 429754. Fax: +66 74 212900. E-mail: jxmky@yahoo.com.cn
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Summary

Pooled sample testing (PST) as a strategy for avoiding testing the majority of individual negative samples has been proposed for screening of diseases in low prevalence areas. There has been no standard guideline for PST in screening of Schistosoma japonicum infection of Yunnan, China. To document the optimum pool size with acceptable sensitivity of PST for screening of Schistosoma japonicum infection in this setting, an experimental pooling of each of 31 positive sera by IHA with various numbers of 24 negative sera was done. The results were used to create a statistical model which was subsequently used for simulation to predict sensitivity of the pooled serum tests in the population with varying prevalence and pool size. We found that to keep the sensitivity of PST above 90%, 1:05 should be the maximum dilution, that is, the optimum pool size should not be greater than 6. Antigen will have rather little interference if the prevalence of infection is low e.g. 1% or the antigen:antibody ratio is 1:100 or below. Pooled serum testing by IHA is an acceptable sensitive method for detecting antibody for Schistosoma japonicum infection in this area.

Keywords

sensitivitypooled serum testingscreeningantibodyschistosomiasis japonicaindirect haemagglutination assay (IHA)
Type
Research Article
Information
Parasitology , Volume 136 , Issue 3 , March 2009 , pp. 267 - 272
Copyright
Copyright © 2009 Cambridge University Press

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References

REFERENCES

Cahoon-Young, B., Chandler, A., Livermore, T., Gaudino, J. and Benjamin, R. (1989). Sensitivity and specificity of pooled versus individual sera in a human immunodeficiency virus antibody prevalence study. Journal Clinical Microbiology 27, 1893–1895.CrossRefGoogle Scholar
Calafat, A. M., Kuklenyik, Z., Caudill, S. P., Reidy, J. A. and Needham, L. L. (2006). Perfluorochemicals in pooled serum samples from United States residents in 2001 and 2002. Environmental Science and Technology 40, 2128–2134.CrossRefGoogle ScholarPubMed
Cao, C. L., Zhu, H. Q., Bao, Z. P. and Zheng, J. (2004). Investigation on acute schistosomiasis in Sichuan province. Chinese Journal of Schistosomiasis Control 16, 387–388.Google Scholar
Chen, J. H., Wen, L. Y., Zhang, J. F., Lu, S. H., Yu, L. L., Ding, J. Z., Shen, L. Y., Wang, T. P., Zhang, S. Q., Chen, G. X., Ye, Y., Zhou, X. N. and Zhou, X. N. (2005). Application of DIGFA, ELISA and IHA for the parallel detection of serum antibodies to Schistosoma japonicum of population in epidemic area of schistosomiasis. Chinese Journal of Zoonoses 21, 776–778.Google Scholar
Chen, M. G. and Feng, Z. (1999). Schistosomiasis control in China (Review articles). Parasitology International 48, 11–19.Google Scholar
Chongsuvivatwong, V. (2008). Analysis of Epidemiological Data Using R and Epicalc. Chanmuang Press, Soi 2, 11 Phetkasem Road, Hat Yai, Songkhla, Thailand.Google Scholar
Engels, D., Wang, L. Y. and Palmer, K. L. (2005). Control of schistosomiasis in China. Acta Tropica 96, 67–68.CrossRefGoogle ScholarPubMed
Fernández, E., Rodrigo, L., García, S., Riestra, S. and Blanco, C. (2006). Hepatitis B surface antigen detection using pooled sera. A cost-benefit analysis. Revista Española de Enfermedades Digestivas: Organo Oficial de la Sociedad Española de Patología Digestiva 98, 112–121.CrossRefGoogle ScholarPubMed
García, Z., Taylor, L., Ruano, A., Pavon, L., Ayerdis, E., Luftig, R. B. and Visona, K. A. (1998). Evaluation of a pooling method for routine anti-HCV screening of blood donors to lower the cost burden on blood banks in countries under development. Journal of Medical Virology 49, 218–222.3.0.CO;2-8>CrossRefGoogle Scholar
Greiner, M. and Gardner, I. A. (2000). Application of diagnostic tests in veterinary epidemiologic studies. Preventive Veterinary Medicine 45, 43–59.CrossRefGoogle ScholarPubMed
Jordan, D. (2005). Simulating the sensitivity of pooled-sample herd tests for fecal Salmonella in cattle. Preventive Veterinary Medicine 70, 59–73.Google Scholar
Kacena, K. A., Quinn, S. B., Howell, M. R., Madico, G. E., Quinn, T. C. and Gaydos, C. A. (1998). Pooling urine samples for ligase chain reaction screening for genital Chlamydia trachomatis infection in asymptomatic women. Clinical Microbiology 36, 481–485.CrossRefGoogle ScholarPubMed
Li, Y., Ross, A. G., Li, Y., He, Y. K., Luo, X. S. and McManus, D. P. (1997). Serological diagnosis of Schistosoma japonicum infections in China. Transactions of the Royal Society of Tropical Medicine and Hygiene 91, 19–21.Google ScholarPubMed
Li, Y. S., Zhao, Z. Y., Ellis, M. and McManus, D. P. (2005). Applications and outcomes of periodic epidemiological surveys for schistosomiasis and related economic evaluation in the People's Republic of China. Acta Tropica 96, 266–275.CrossRefGoogle ScholarPubMed
Muñoz-Zanzi, C. A., Johnson, W. O., Thurmond, M. C. and Hietala, S. K. (2000). Pooled-sample testing as a herd-screening tool for detection of bovine viral diarrhea virus persistently infected cattle. Journal of Veterinary Diagnostic Investigation 12, 195–203.Google Scholar
Muñoz-Zanzi, C., Thurmond, M., Hietala, S. and Johnson, W. (2006). Factors affecting sensitivity and specificity of pooled-sample testing for diagnosis of low prevalence infections. Preventive Veterinary Medicine 74, 309–322.Google Scholar
Novack, L., Sarov, B., Goldman-Levi, R., Yahalom, V., Safi, J., Soliman, H., Orgel, M., Yaari, A., Galai, N., Pliskin, J. S. and Shinar, E. (2008). Impact of pooling on accuracy of hepatitis B virus surface antigen screening of blood donations. Transactions of the Royal Society of Tropical Medicine and Hygiene 102, 787–792.CrossRefGoogle ScholarPubMed
Polman, K., Stelma, F. F., Gryseels, B., Van Dam, G. J., Talla, I., Niang, M., Van, L. L. and Deelder, A. M. (1995). Epidemiologic application of circulating antigen detection in a recent schistosoma mansoni focus in northern Senegal. American Journal of Tropical Medicine and Hygiene 53, 152–157.CrossRefGoogle Scholar
R Development Core Team (2008). R:A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria.Google Scholar
Raboud, J. M., Major, C., Sherlock, C. and O'Shaughnessy, M. V. (1996). The effects of pooling serum samples from seroconverting individuals or individuals with end stage disease for HIV antibody testing:a comparison of four screen tests and three pool sizes. Serodiagnosis and Immunotherapy in Infectious Disease 8, 19–24.CrossRefGoogle Scholar
Rovira, A., Cano, J. P. and Muñoz-Zanzi, C. (2008). Feasibility of pooled-sample testing for the detection of porcine reproductive and respiratory syndrome virus antibodies on serum samples by ELISA. Veterinary Microbiology 130, 60–68.CrossRefGoogle ScholarPubMed
Sarov, B., Novack, L., Beer, N., Safi, J., Soliman, H., Pliskin, J. S., Litvak, E., Yaari, A. and Shinar, E. (2007). Feasibility and cost-benefit of implementing pooled screening for HCVAg in small blood bank settings. Transfusion Medicine 17, 479–487.Google Scholar
Singer, R. S., Cooke, C. L., Maddox, C. W., Isaacson, R. E. and Wallace, R. L. (2006). Use of pooled samples for the detection of Salmonella in feces by polymerase chain reaction. Journal of Veterinary Diagnostic Investigation 18, 319–325.Google Scholar
Soroka, S. D., Granade, T. C., Phillips, S. and Parekh, B. (2003). The use of simple, rapid tests to detect antibodies to human immunodeficiency virus types 1 and 2 in pooled serum specimens. Journal of Clinical Virology 27, 90–96.Google Scholar
Utzinger, J., Zhou, X. N., Chen, M. G. and Bergquist, R. (2005). Conquering schistosomiasis in China: the long march. Acta Tropica 96, 69–96.Google Scholar
Van, L. L., Polderman, A. M., Visser, L. G., Verwey, J. J. and Deelder, A. M. (1997). Detection of the circulating antigens CAA and CCA in a group of Dutch travellers with acute schistosomiasis. Tropical Medicine & International Health 2, 551–557.Google Scholar
Wells, S. J., Godden, S. M., Lindeman, C. J. and Collins, J. E. (2003). Evaluation of bacteriologic culture of individual and pooled fecal samples for detection of Mycobacterium paratuberculosis in dairy cattle herds. Journal of the American Veterinary Medical Association 223, 1022–1025.Google Scholar
Westreich, D. J., Hudgens, M. G., Fiscus, S. A. and Pilcher, C. D. (2008). Optimizing screening for acute human immunodeficiency virus infection with pooled nucleic acid amplification tests. Journal of Clinical Microbiology 46, 1785–1799.Google Scholar
Wu, G. L. (2005 a). Human Parasitology. Beijing: Publishing House for Pepole's Health (in Chinese).Google Scholar
Wu, G. L. (2005 b). Immunodiagnosis of Schistosoma japonicum infection in China: review and prospects. Zhongguo Ji. Sheng Chong. Xue. Yu Ji. Sheng Chong. Bing. Za Zhi. 23, 323–328.Google Scholar
Xu, J., Chen, N. G., Feng, T., Wang, E. M., Wu, X. H., Chen, H. G., Wang, T. P., Zhou, X. N. and Zheng, J. (2007). Effectiveness of routinely used assays for the diagnosis of schistosomiasis japonica in the field. Zhongguo Ji. Sheng Chong. Xue. Yu Ji. Sheng Chong. Bing. Za Zhi. 25, 175–179.Google ScholarPubMed
Yang, Y. Z., Yang, Y. Z., Duan, S. S., Yang, G. H.I. and Li, X. Y. (2004). Investigation on acute schistosomiasis break out in Shangdeng village, Dali city, 2003. Chinese Journal of Schistosomiasis Control 16, 273.Google Scholar
Zhang, W. and Wong, C. M. (2003). Evaluation of the 1992–1999 world bank schistosomiasis control project in China. Acta Tropica 85, 303–313.Google Scholar
Zhao, G. M., Zhao, Q., Jiang, Q. W., Chen, X. Y., Wang, L. Y. and Yuan, H. C. (2005). Surveillance for schistosomiasis japonica in China from 2000 to 2003. Acta Tropica 96, 288–295.Google Scholar
Zheng, J. (2005). The strategy of schistosomiasis prevention and control must persist in social interruption mainly in China. Foreign Medical Sciences Epidemiology Lemology 32, 4–7.Google Scholar
Zhou, X. N., Guo, J. G., Wu, X. H., Jiang, Q. W., Zheng, J., Dang, H., Wang, X. H., Xu, J., Zhu, H. Q., Wu, G. L., Li, Y. S., Xu, X. J., Chen, H. G., Wang, T. P., Zhu, Y. C., Qiu, D. C., Dong, X. Q., Zhao, G. M., Zhang, S. J., Zhao, N. Q., Xia, G., Wang, L. Y., Zhang, S. Q., Lin, D. D., Chen, M. G. and Hao, Y. (2007). Epidemiology of Schistosomiasis in the People's Republic of China, 2004. Emerging Infectious Diseases 13, 1470–1476.Google Scholar
Zhou, X. N., Wang, L. Y., Chen, M. G., Wu, X. H., Jiang, Q. W., Chen, X. Y., Zheng, J. and Utzinger, J. (2005). The public health significance and control of schistosomiasis in China then and now. Acta Tropica 96, 97–105.Google Scholar
Zhu, Y. C. (2005). Immunodiagnosis and its role in schistosomiasis control in China: a review. Acta Tropica 96, 130–136.CrossRefGoogle Scholar