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The use of DNA markers to map anthelmintic resistance loci in an intraspecific cross of Haemonchus contortus

Published online by Cambridge University Press:  16 October 2009

P. W. HUNT ,
A. C. KOTZE ,
M. R. KNOX ,
L. J. ANDERSON ,
J. McNALLY  and
L. F. LE JAMBRE
P. W. HUNT*
Affiliation:
CSIRO, F.D. McMaster Laboratory, Chiswick, New England Highway, Armidale, N.S.W.2350, Australia
A. C. KOTZE
Affiliation:
CSIRO Livestock Industries, Brisbane, Australia
M. R. KNOX
Affiliation:
CSIRO, F.D. McMaster Laboratory, Chiswick, New England Highway, Armidale, N.S.W.2350, Australia
L. J. ANDERSON
Affiliation:
CSIRO, F.D. McMaster Laboratory, Chiswick, New England Highway, Armidale, N.S.W.2350, Australia
J. McNALLY
Affiliation:
CSIRO, F.D. McMaster Laboratory, Chiswick, New England Highway, Armidale, N.S.W.2350, Australia
L. F. LE JAMBRE
Affiliation:
CSIRO, F.D. McMaster Laboratory, Chiswick, New England Highway, Armidale, N.S.W.2350, Australia
*
*Corresponding author: CSIRO, F.D. McMaster Laboratory, Chiswick, New England Highway, Armidale, N.S.W.2350, Australia. Tel: +61 2 67761321. Fax: +61 2 67761333. E-mail: Peter.Hunt@csiro.au
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Summary

The use of DNA markers to track the development of anthelmintic resistance in parasites of livestock would allow informed choices for the management of this important problem. We describe a genetic mapping approach for the discovery of DNA markers for anthelmintic resistance in Haemonchus contortus. We crossed a multi-drug resistant field isolate of H. contortus with a well-characterized laboratory strain susceptible to 4 drug classes. The F2 were separately selected with 5 anthelmintics from 4 drug classes, producing drug-resistant populations carrying gene variants derived from both the field isolate and the laboratory strain. Individual F2 worms were analysed using amplicon length polymorphisms (ALPs). We looked for field isolate alleles over- or under-represented in F2 populations compared to the unselected F2 and/or the laboratory strain. The data we obtained suggest that marker association can be used to link neutral markers with resistance, but also that more markers and perhaps more inbred laboratory strains would make the procedure more likely to succeed.

Keywords

Haemonchus contortusdrug resistancepopulation geneticsDNA markersAustralia
Type
Research Article
Information
Parasitology , Volume 137 , Issue 4 , April 2010 , pp. 705 - 717
Copyright
Copyright © Cambridge University Press 2009

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