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Development of EST-SSR markers for an endangered plant species, Camellia fascicularis (Theaceae)

Published online by Cambridge University Press:  16 March 2023

Luyao Ma ,
Cheng Liu ,
Jing Xin ,
Bin Li ,
Junrong Tang ,
Yaxuan Xin ,
Peng Ye ,
Peiyao Xin Open the ORCID record for Peiyao Xin [Opens in a new window]  and
Bin Bai
Luyao Ma
Affiliation:
Southwest Research Center for Landscape Architecture Engineering, State Forestry and Grassland Administration, Southwest Forestry University, Kunming 650224, China Key Laboratory of Biodiversity Conservation in Southwest Region of State Forestry and Grassland Administration, Southwest Forestry University, Kunming, Yunnan 650224, China
Cheng Liu
Affiliation:
Southwest Research Center for Landscape Architecture Engineering, State Forestry and Grassland Administration, Southwest Forestry University, Kunming 650224, China Key Laboratory of Biodiversity Conservation in Southwest Region of State Forestry and Grassland Administration, Southwest Forestry University, Kunming, Yunnan 650224, China
Jing Xin
Affiliation:
Key Laboratory of Biodiversity Conservation in Southwest Region of State Forestry and Grassland Administration, Southwest Forestry University, Kunming, Yunnan 650224, China
Bin Li
Affiliation:
Bijie Region Forestry Science Research Institute, Bijie 551700, China
Junrong Tang
Affiliation:
Key Laboratory of Biodiversity Conservation in Southwest Region of State Forestry and Grassland Administration, Southwest Forestry University, Kunming, Yunnan 650224, China
Yaxuan Xin
Affiliation:
Key Laboratory of Biodiversity Conservation in Southwest Region of State Forestry and Grassland Administration, Southwest Forestry University, Kunming, Yunnan 650224, China
Peng Ye
Affiliation:
Southwest Research Center for Landscape Architecture Engineering, State Forestry and Grassland Administration, Southwest Forestry University, Kunming 650224, China Key Laboratory of Biodiversity Conservation in Southwest Region of State Forestry and Grassland Administration, Southwest Forestry University, Kunming, Yunnan 650224, China
Peiyao Xin*
Affiliation:
Southwest Research Center for Landscape Architecture Engineering, State Forestry and Grassland Administration, Southwest Forestry University, Kunming 650224, China Key Laboratory of Biodiversity Conservation in Southwest Region of State Forestry and Grassland Administration, Southwest Forestry University, Kunming, Yunnan 650224, China
Bin Bai*
Affiliation:
Wheat Research Institute, Gansu Academy of Agricultural Sciences, Lanzhou 730070, China
*
Authors for correspondence: Peiyao Xin, E-mail: xpytgyx@163.com; Bin Bai, E-mail: baibingaas@gsagr.cn
Authors for correspondence: Peiyao Xin, E-mail: xpytgyx@163.com; Bin Bai, E-mail: baibingaas@gsagr.cn
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Abstract

The plant Camellia fascicularis, belonging to family Theaceae, has high ornamental and medicinal value, and rare gene resources for genetic improvement of Camellia crops, but is currently threatened with extinction because of the unique and extremely small wild populations. Molecular markers have clarified the wild plant species’ genetic diversity structure, new gene resources and relationship with crops. This will be beneficial for conservation of these valuable crop-related wild species and crop improvement. In this study, we identified 95,979 microsatellite loci from 155,011 transcriptome unigenes, and developed 14 polymorphic expressed sequence tag-derived simple sequence repeat (EST-SSR) microsatellite markers for C. fascicularis. The number of alleles (Na) per locus was 2–8 with a mean of 4.86. The genetic diversity of 40 individuals from four natural populations of C. fascicularis was analysed using these polymorphic markers. The number of alleles (Na) for EST-SSR ranged from 2 to 5, with the expected heterozygosities (He) and observed heterozygosities (Ho) in all loci ranging from 0.183 to 0.683, and from 0.201 to 0.700, respectively, implying a rich genetic variation present in wild C. fascicularis populations. Moreover, the phylogenetic analysis among four populations, using the 14 EST-SSR markers developed in this study, grouped 40 individuals into three groups, which coincide with their geographic distribution. These results showed that 14 EST-SSR markers are available for the analysis of genetic variation in C. fascicularis populations and genetic improvement of new Camellias cultivars by interspecific hybridization, and are beneficial for conservation of the endangered species.

Keywords

Camellia fascicularisEST-SSRgenetic diversityTheaceaetranscriptome sequencing
Type
Research Article
Information
Plant Genetic Resources , Volume 20 , Issue 4 , August 2022 , pp. 263 - 269
Copyright
Copyright © The Author(s), 2023. Published by Cambridge University Press on behalf of NIAB

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