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Flavonoid content in seeds of guar germplasm using HPLC

Published online by Cambridge University Press:  01 August 2007

M. L. Wang  and
J. B. Morris
M. L. Wang*
Affiliation:
USDA-ARS, Plant Genetic Resources Conservation Unit, 1109 Experiment Street, Griffin, GA 30223, USA
J. B. Morris
Affiliation:
USDA-ARS, Plant Genetic Resources Conservation Unit, 1109 Experiment Street, Griffin, GA 30223, USA
*
*Corresponding author. E-mail: mwang@ars-grin.gov
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Abstract

Legume flavonoids have received considerable attention due to their beneficial effects on human health. Flavonoid content in the seeds of 36 germplasm accessions of guar [Cyamopsis tetragonoloba (L.) Taub.] was quantified by high-performance liquid chromatography (HPLC). On a per 100 g basis, the seed contained, on average, 1.114 mg (0–2.355) daidzein, 0.700 mg (0–1.685) genistein, 0.553 mg (0–1.250) quercetin and 14.460 mg (10.70–19.82) kaempferol. A significant positive correlation was observed between the content of daidzein and genistein; however, significant negative correlations existed between the content of kaempferol and daidzein and of kaempferol and genistein. Compared to soybean seeds, guar seeds contained very low amounts of daidzein and genistein, but a high amount of kaempferol. The information about the levels of flavonoids in guar seeds will be useful to breeding programmes for improving guar seed quality. Furthermore, the high kaempferol content of guar seeds may expand its nutraceutical and pharmaceutical utilization.

Keywords

flavonoid contentgermplasmguarhigh-performance liquid chromatography
Type
In Brief
Information
Plant Genetic Resources , Volume 5 , Issue 2 , August 2007 , pp. 96 - 99
Copyright
Copyright © NIAB 2007

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References

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